Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision

Abstract Environmental stressors, gonadal degenerative diseases and tumour development can significantly alter the oocyte physiology, and species fertility and fitness. To expand the molecular understanding about oocyte degradation, we isolated several spliced variants of Japanese anchovy hatching e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tapas Chakraborty, Sipra Mohapatra, Megumi Tobayama, Kayoko Ohta, Yong-Woon Ryu, Yukinori Kazeto, Kohei Ohta, Linyan Zhou, Yoshitaka Nagahama, Takahiro Matsubara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18886302e23c4fdeba8cdbb7170ba575
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:18886302e23c4fdeba8cdbb7170ba575
record_format	dspace
spelling	oai:doaj.org-article:18886302e23c4fdeba8cdbb7170ba5752021-12-02T11:52:35ZHatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision10.1038/s41598-017-03314-72045-2322https://doaj.org/article/18886302e23c4fdeba8cdbb7170ba5752017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03314-7https://doaj.org/toc/2045-2322Abstract Environmental stressors, gonadal degenerative diseases and tumour development can significantly alter the oocyte physiology, and species fertility and fitness. To expand the molecular understanding about oocyte degradation, we isolated several spliced variants of Japanese anchovy hatching enzymes (AcHEs; ovastacin homologue) 1 and 2, and analysed their potential in oocyte sustenance. Particularly, AcHE1b, an ovary-specific, steroid-regulated, methylation-dependent, stress-responsive isoform, was neofunctionalized to regulate autophagic oocyte degeneration. AcHE1a and 2 triggered apoptotic degeneration in vitellogenic and mature oocytes, respectively. Progesterone, starvation, and high temperature elevated the total degenerating oocyte population and AcHE1b transcription by hyper-demethylation. Overexpression, knockdown and intracellular zinc ion chelation study confirmed the functional significance of AcHE1b in autophagy induction, possibly to mitigate the stress effects in fish, via ion-homeostasis. Our finding chronicles the importance of AcHEs in stress-influenced apoptosis/autophagy cell fate decision and may prove significant in reproductive failure assessments, gonadal health maintenance and ovarian degenerative disease therapy.Tapas ChakrabortySipra MohapatraMegumi TobayamaKayoko OhtaYong-Woon RyuYukinori KazetoKohei OhtaLinyan ZhouYoshitaka NagahamaTakahiro MatsubaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tapas Chakraborty Sipra Mohapatra Megumi Tobayama Kayoko Ohta Yong-Woon Ryu Yukinori Kazeto Kohei Ohta Linyan Zhou Yoshitaka Nagahama Takahiro Matsubara Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
description	Abstract Environmental stressors, gonadal degenerative diseases and tumour development can significantly alter the oocyte physiology, and species fertility and fitness. To expand the molecular understanding about oocyte degradation, we isolated several spliced variants of Japanese anchovy hatching enzymes (AcHEs; ovastacin homologue) 1 and 2, and analysed their potential in oocyte sustenance. Particularly, AcHE1b, an ovary-specific, steroid-regulated, methylation-dependent, stress-responsive isoform, was neofunctionalized to regulate autophagic oocyte degeneration. AcHE1a and 2 triggered apoptotic degeneration in vitellogenic and mature oocytes, respectively. Progesterone, starvation, and high temperature elevated the total degenerating oocyte population and AcHE1b transcription by hyper-demethylation. Overexpression, knockdown and intracellular zinc ion chelation study confirmed the functional significance of AcHE1b in autophagy induction, possibly to mitigate the stress effects in fish, via ion-homeostasis. Our finding chronicles the importance of AcHEs in stress-influenced apoptosis/autophagy cell fate decision and may prove significant in reproductive failure assessments, gonadal health maintenance and ovarian degenerative disease therapy.
format	article
author	Tapas Chakraborty Sipra Mohapatra Megumi Tobayama Kayoko Ohta Yong-Woon Ryu Yukinori Kazeto Kohei Ohta Linyan Zhou Yoshitaka Nagahama Takahiro Matsubara
author_facet	Tapas Chakraborty Sipra Mohapatra Megumi Tobayama Kayoko Ohta Yong-Woon Ryu Yukinori Kazeto Kohei Ohta Linyan Zhou Yoshitaka Nagahama Takahiro Matsubara
author_sort	Tapas Chakraborty
title	Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
title_short	Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
title_full	Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
title_fullStr	Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
title_full_unstemmed	Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
title_sort	hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/18886302e23c4fdeba8cdbb7170ba575
work_keys_str_mv	AT tapaschakraborty hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT sipramohapatra hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT megumitobayama hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT kayokoohta hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT yongwoonryu hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT yukinorikazeto hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT koheiohta hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT linyanzhou hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT yoshitakanagahama hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision AT takahiromatsubara hatchingenzymesdisruptaberrantgonadaldegenerationbytheautophagyapoptosiscellfatedecision
_version_	1718394968773492736

Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision

Ejemplares similares