An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens

We report a new competitive lateral flow immunoassay (LFIA) for hapten detection, which uses antibodies to bind antigens from a sample and form a coloured immune complex due to artificial antigens conjugated with gold nanoparticles; these were intercepted with SPA. Here, 2,4-dichlorophenoxyacetic ac...

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Autores principales:	Yaning Sun, Suzhen Yang, Jifei Yang, Xiaofei Hu, Qiang Wei, Yao Wang, Yunrui Xing, Linlin Chen, Xinxin Chen, Ruiguang Deng, Gaiping Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	sensitive enhancement competitive lateral flow immunoassay test strip gm 2,4-d Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1891fa5f185c473bbd31d6e682332152
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spelling	oai:doaj.org-article:1891fa5f185c473bbd31d6e6823321522021-11-04T15:00:41ZAn accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens0954-01051465-344310.1080/09540105.2021.1989382https://doaj.org/article/1891fa5f185c473bbd31d6e6823321522021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2021.1989382https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443We report a new competitive lateral flow immunoassay (LFIA) for hapten detection, which uses antibodies to bind antigens from a sample and form a coloured immune complex due to artificial antigens conjugated with gold nanoparticles; these were intercepted with SPA. Here, 2,4-dichlorophenoxyacetic acid (2,4-D) and gentamicin (GM) were selected as model analytes. The visual limit of detection of the 2,4-D strip was 25 ng/mL in tap water; the GM strip was 3.13 ng/mL in milk. This approach exhibits more than a 40-fold lower detection limit for 2,4-D and 3.2-fold lower for GM, which is compared to traditional LFIA using the same reagents. This approach can accurately control the amount of antibody, protect the activity of antibody and enhance the detection signal, thus improving the sensitivity of the LFIA and providing more and more sensitive methods for the development of LFIAs. Highlights This study developed a new method of LFIA that could improve the sensitivity of LFIA without adding any reagent. The antibodies are unlabelled; thus, the immunological properties of the antibodies are not destroyed by antibody folding during the labelling process. Sufficient gold-labelled antigens ensure that each antibody bound to the test line can combine two gold nanoparticles, thereby amplifying the overall signal.Yaning SunSuzhen YangJifei YangXiaofei HuQiang WeiYao WangYunrui XingLinlin ChenXinxin ChenRuiguang DengGaiping ZhangTaylor & Francis Grouparticlesensitive enhancementcompetitive lateral flow immunoassaytest stripgm2,4-dAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 32, Iss 1, Pp 766-777 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sensitive enhancement competitive lateral flow immunoassay test strip gm 2,4-d Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	sensitive enhancement competitive lateral flow immunoassay test strip gm 2,4-d Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Yaning Sun Suzhen Yang Jifei Yang Xiaofei Hu Qiang Wei Yao Wang Yunrui Xing Linlin Chen Xinxin Chen Ruiguang Deng Gaiping Zhang An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
description	We report a new competitive lateral flow immunoassay (LFIA) for hapten detection, which uses antibodies to bind antigens from a sample and form a coloured immune complex due to artificial antigens conjugated with gold nanoparticles; these were intercepted with SPA. Here, 2,4-dichlorophenoxyacetic acid (2,4-D) and gentamicin (GM) were selected as model analytes. The visual limit of detection of the 2,4-D strip was 25 ng/mL in tap water; the GM strip was 3.13 ng/mL in milk. This approach exhibits more than a 40-fold lower detection limit for 2,4-D and 3.2-fold lower for GM, which is compared to traditional LFIA using the same reagents. This approach can accurately control the amount of antibody, protect the activity of antibody and enhance the detection signal, thus improving the sensitivity of the LFIA and providing more and more sensitive methods for the development of LFIAs. Highlights This study developed a new method of LFIA that could improve the sensitivity of LFIA without adding any reagent. The antibodies are unlabelled; thus, the immunological properties of the antibodies are not destroyed by antibody folding during the labelling process. Sufficient gold-labelled antigens ensure that each antibody bound to the test line can combine two gold nanoparticles, thereby amplifying the overall signal.
format	article
author	Yaning Sun Suzhen Yang Jifei Yang Xiaofei Hu Qiang Wei Yao Wang Yunrui Xing Linlin Chen Xinxin Chen Ruiguang Deng Gaiping Zhang
author_facet	Yaning Sun Suzhen Yang Jifei Yang Xiaofei Hu Qiang Wei Yao Wang Yunrui Xing Linlin Chen Xinxin Chen Ruiguang Deng Gaiping Zhang
author_sort	Yaning Sun
title	An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
title_short	An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
title_full	An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
title_fullStr	An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
title_full_unstemmed	An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
title_sort	accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1891fa5f185c473bbd31d6e682332152
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An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens

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