Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model

Abstract Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucid...

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Autores principales:	Hongxia Zhao, Xiaoqun Li, Dianying Zhang, Haiyan Chen, Yufan Chao, Kaiwen Wu, Xin Dong, Jiacan Su
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Osteoporosis Mice Postmenopausal Osteoporosis Glycerophospholipids Bone Volume/total Volume (BV/TV) Lipidomic Analysis Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1897ce3a0b6f4955bd4551d6495da09f
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spelling	oai:doaj.org-article:1897ce3a0b6f4955bd4551d6495da09f2021-12-02T15:08:57ZIntegrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model10.1038/s41598-018-34574-62045-2322https://doaj.org/article/1897ce3a0b6f4955bd4551d6495da09f2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34574-6https://doaj.org/toc/2045-2322Abstract Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucidated to date. In this study, bilateral ovariectomy was performed to establish an OVX mouse model of osteoporosis. UPLC-Q-TOF-MS-based lipidomics in combination with metabolomics were used to analyze the femur tissue of osteoporosis mice. We found that 11 polar metabolites and 93 lipid metabolites were significantly changed and were involved in amino acid metabolism, nucleotide metabolism and lipid metabolism. Among the lipids, fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterols showed robust changes. These results revealed that several metabolic disorders caused by changes in the hormone levels in OVX, especially disordered lipid metabolism, are closely related to the imbalance between bone resorption and formation and may underlie the development of PMOP. The data generated via lipidomics and metabolomics presented in this study shows good applicability and wide coverage in the construction of the metabolic profile of bone tissue. Therefore, this approach may provide the pathway focusing and data support at the metabolite level for the in-depth mechanism of PMOP.Hongxia ZhaoXiaoqun LiDianying ZhangHaiyan ChenYufan ChaoKaiwen WuXin DongJiacan SuNature PortfolioarticleOsteoporosis MicePostmenopausal OsteoporosisGlycerophospholipidsBone Volume/total Volume (BV/TV)Lipidomic AnalysisMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteoporosis Mice Postmenopausal Osteoporosis Glycerophospholipids Bone Volume/total Volume (BV/TV) Lipidomic Analysis Medicine R Science Q
spellingShingle	Osteoporosis Mice Postmenopausal Osteoporosis Glycerophospholipids Bone Volume/total Volume (BV/TV) Lipidomic Analysis Medicine R Science Q Hongxia Zhao Xiaoqun Li Dianying Zhang Haiyan Chen Yufan Chao Kaiwen Wu Xin Dong Jiacan Su Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
description	Abstract Osteoporosis, characterized by bone mass reduction and increased fractures, has become a global health problem that seriously affects the health of people, especially postmenopausal women; however, the current pathogenesis of postmenopausal osteoporosis (PMOP) has not been thoroughly elucidated to date. In this study, bilateral ovariectomy was performed to establish an OVX mouse model of osteoporosis. UPLC-Q-TOF-MS-based lipidomics in combination with metabolomics were used to analyze the femur tissue of osteoporosis mice. We found that 11 polar metabolites and 93 lipid metabolites were significantly changed and were involved in amino acid metabolism, nucleotide metabolism and lipid metabolism. Among the lipids, fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterols showed robust changes. These results revealed that several metabolic disorders caused by changes in the hormone levels in OVX, especially disordered lipid metabolism, are closely related to the imbalance between bone resorption and formation and may underlie the development of PMOP. The data generated via lipidomics and metabolomics presented in this study shows good applicability and wide coverage in the construction of the metabolic profile of bone tissue. Therefore, this approach may provide the pathway focusing and data support at the metabolite level for the in-depth mechanism of PMOP.
format	article
author	Hongxia Zhao Xiaoqun Li Dianying Zhang Haiyan Chen Yufan Chao Kaiwen Wu Xin Dong Jiacan Su
author_facet	Hongxia Zhao Xiaoqun Li Dianying Zhang Haiyan Chen Yufan Chao Kaiwen Wu Xin Dong Jiacan Su
author_sort	Hongxia Zhao
title	Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
title_short	Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
title_full	Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
title_fullStr	Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
title_full_unstemmed	Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model
title_sort	integrative bone metabolomics—lipidomics strategy for pathological mechanism of postmenopausal osteoporosis mouse model
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1897ce3a0b6f4955bd4551d6495da09f
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_version_	1718387923093553152

Integrative Bone Metabolomics—Lipidomics Strategy for Pathological Mechanism of Postmenopausal Osteoporosis Mouse Model

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