Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis

Abstract Chronic pancreatitis (CP) is a fibro-inflammatory syndrome in individuals who develop persistent pathological responses to parenchymal injury or stress. Novel therapeutic or dietary interventions that could lessen inflammation in this disease could significantly improve quality of life in p...

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Autores principales:	Debasmita Mukherjee, Mallory J. DiVincenzo, Molly Torok, Fouad Choueiry, Rahul J. Kumar, Anna Deems, Jenna L. Miller, Alice Hinton, Connor Geraghty, Jose Angel Maranon, Samuel K. Kulp, Christopher Coss, William E. Carson, Darwin L. Conwell, Phil A. Hart, Jessica L. Cooperstone, Thomas A. Mace
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/189cf0125a7b4943bdf76c48d08a9636
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spelling	oai:doaj.org-article:189cf0125a7b4943bdf76c48d08a96362021-12-02T12:33:05ZSoy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis10.1038/s41598-020-78762-92045-2322https://doaj.org/article/189cf0125a7b4943bdf76c48d08a96362020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78762-9https://doaj.org/toc/2045-2322Abstract Chronic pancreatitis (CP) is a fibro-inflammatory syndrome in individuals who develop persistent pathological responses to parenchymal injury or stress. Novel therapeutic or dietary interventions that could lessen inflammation in this disease could significantly improve quality of life in patients with CP. Complex dietary foods like soy and tomatoes are composed of active metabolites with anti-inflammatory effects. Data from our group reports that bioactive agents in soy and tomatoes can reduce pro-inflammatory cytokines and suppressive immune populations. Additionally, our team has developed a novel soy-tomato juice currently being studied in healthy individuals with no toxicities, and good compliance and bioavailability. Thus, we hypothesize that administration of a soy-tomato enriched diet can reduce inflammation and severity of CP. C57BL/6 mice were injected intraperitoneally with 50 μg/kg caeurlein (7 hourly injections, twice weekly) for 6 weeks to induce CP. After 4 weeks of caerulein injections, mice were administered a control or a soy-tomato enriched diet for 2 weeks. Disease severity was measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis. Serum lipase and amylase levels were analyzed at the end of the study. Inflammatory factors in the serum and pancreas, and immune populations in the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respectively. Infra-red (IR) sensing of mice was used to monitor spontaneous activity and distress of mice. Mice fed a soy-tomato enriched diet had a significantly reduced level of inflammation and severity of CP (p = 0.032) compared to mice administered a control diet with restored serum lipase and amylase levels (p < 0.05). Mice with CP fed a soy-tomato diet had a reduction in inflammatory factors (TNF-α, IL-1β, IL-5) and suppressive immune populations (myeloid-derived suppressor cells; MDSC) compared to control diet fed mice (p < 0.05). Infra-red sensing to monitor spontaneous activity of mice showed that soy-tomato enriched diet improved total activity and overall health of mice with CP (p = 0.055) and CP mice on a control diet were determined to spend more time at rest (p = 0.053). These pre-clinical results indicate that a soy-tomato enriched diet may be a novel treatment approach to reduce inflammation and pain in patients with CP.Debasmita MukherjeeMallory J. DiVincenzoMolly TorokFouad ChoueiryRahul J. KumarAnna DeemsJenna L. MillerAlice HintonConnor GeraghtyJose Angel MaranonSamuel K. KulpChristopher CossWilliam E. CarsonDarwin L. ConwellPhil A. HartJessica L. CooperstoneThomas A. MaceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Debasmita Mukherjee Mallory J. DiVincenzo Molly Torok Fouad Choueiry Rahul J. Kumar Anna Deems Jenna L. Miller Alice Hinton Connor Geraghty Jose Angel Maranon Samuel K. Kulp Christopher Coss William E. Carson Darwin L. Conwell Phil A. Hart Jessica L. Cooperstone Thomas A. Mace Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
description	Abstract Chronic pancreatitis (CP) is a fibro-inflammatory syndrome in individuals who develop persistent pathological responses to parenchymal injury or stress. Novel therapeutic or dietary interventions that could lessen inflammation in this disease could significantly improve quality of life in patients with CP. Complex dietary foods like soy and tomatoes are composed of active metabolites with anti-inflammatory effects. Data from our group reports that bioactive agents in soy and tomatoes can reduce pro-inflammatory cytokines and suppressive immune populations. Additionally, our team has developed a novel soy-tomato juice currently being studied in healthy individuals with no toxicities, and good compliance and bioavailability. Thus, we hypothesize that administration of a soy-tomato enriched diet can reduce inflammation and severity of CP. C57BL/6 mice were injected intraperitoneally with 50 μg/kg caeurlein (7 hourly injections, twice weekly) for 6 weeks to induce CP. After 4 weeks of caerulein injections, mice were administered a control or a soy-tomato enriched diet for 2 weeks. Disease severity was measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis. Serum lipase and amylase levels were analyzed at the end of the study. Inflammatory factors in the serum and pancreas, and immune populations in the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respectively. Infra-red (IR) sensing of mice was used to monitor spontaneous activity and distress of mice. Mice fed a soy-tomato enriched diet had a significantly reduced level of inflammation and severity of CP (p = 0.032) compared to mice administered a control diet with restored serum lipase and amylase levels (p < 0.05). Mice with CP fed a soy-tomato diet had a reduction in inflammatory factors (TNF-α, IL-1β, IL-5) and suppressive immune populations (myeloid-derived suppressor cells; MDSC) compared to control diet fed mice (p < 0.05). Infra-red sensing to monitor spontaneous activity of mice showed that soy-tomato enriched diet improved total activity and overall health of mice with CP (p = 0.055) and CP mice on a control diet were determined to spend more time at rest (p = 0.053). These pre-clinical results indicate that a soy-tomato enriched diet may be a novel treatment approach to reduce inflammation and pain in patients with CP.
format	article
author	Debasmita Mukherjee Mallory J. DiVincenzo Molly Torok Fouad Choueiry Rahul J. Kumar Anna Deems Jenna L. Miller Alice Hinton Connor Geraghty Jose Angel Maranon Samuel K. Kulp Christopher Coss William E. Carson Darwin L. Conwell Phil A. Hart Jessica L. Cooperstone Thomas A. Mace
author_facet	Debasmita Mukherjee Mallory J. DiVincenzo Molly Torok Fouad Choueiry Rahul J. Kumar Anna Deems Jenna L. Miller Alice Hinton Connor Geraghty Jose Angel Maranon Samuel K. Kulp Christopher Coss William E. Carson Darwin L. Conwell Phil A. Hart Jessica L. Cooperstone Thomas A. Mace
author_sort	Debasmita Mukherjee
title	Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
title_short	Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
title_full	Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
title_fullStr	Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
title_full_unstemmed	Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
title_sort	soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/189cf0125a7b4943bdf76c48d08a9636
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Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis

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