cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

Abstract Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (G...

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Autores principales:	Carolina Inda, Juan José Bonfiglio, Paula A. dos Santos Claro, Sergio A. Senin, Natalia G. Armando, Jan M. Deussing, Susana Silberstein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18a1ff3e3a154fb0897d2fe370ba4859
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spelling	oai:doaj.org-article:18a1ff3e3a154fb0897d2fe370ba48592021-12-02T11:52:35ZcAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells10.1038/s41598-017-02021-72045-2322https://doaj.org/article/18a1ff3e3a154fb0897d2fe370ba48592017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02021-7https://doaj.org/toc/2045-2322Abstract Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes.Carolina IndaJuan José BonfiglioPaula A. dos Santos ClaroSergio A. SeninNatalia G. ArmandoJan M. DeussingSusana SilbersteinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Inda Juan José Bonfiglio Paula A. dos Santos Claro Sergio A. Senin Natalia G. Armando Jan M. Deussing Susana Silberstein cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
description	Abstract Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes.
format	article
author	Carolina Inda Juan José Bonfiglio Paula A. dos Santos Claro Sergio A. Senin Natalia G. Armando Jan M. Deussing Susana Silberstein
author_facet	Carolina Inda Juan José Bonfiglio Paula A. dos Santos Claro Sergio A. Senin Natalia G. Armando Jan M. Deussing Susana Silberstein
author_sort	Carolina Inda
title	cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_short	cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_full	cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_fullStr	cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_full_unstemmed	cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_sort	camp-dependent cell differentiation triggered by activated crhr1 in hippocampal neuronal cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/18a1ff3e3a154fb0897d2fe370ba4859
work_keys_str_mv	AT carolinainda campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT juanjosebonfiglio campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT paulaadossantosclaro campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT sergioasenin campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT nataliagarmando campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT janmdeussing campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT susanasilberstein campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells
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cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

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