Effect of the Min system on timing of cell division in Escherichia coli.

In Escherichia coli the Min protein system plays an important role in positioning the division site. We show that this system also has an effect on timing of cell division. We do this in a quantitative way by measuring the cell division waiting time (defined as time difference between appearance of...

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Autores principales:	Shuxin Jia, Daniela Keilberg, Edina Hot, Martin Thanbichler, Lotte Søgaard-Andersen, Peter Lenz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18a8305ca809482884c7cc699e17137d
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id	oai:doaj.org-article:18a8305ca809482884c7cc699e17137d
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spelling	oai:doaj.org-article:18a8305ca809482884c7cc699e17137d2021-11-25T06:06:10ZEffect of the Min system on timing of cell division in Escherichia coli.1932-620310.1371/journal.pone.0103863https://doaj.org/article/18a8305ca809482884c7cc699e17137d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25090009/?tool=EBIhttps://doaj.org/toc/1932-6203In Escherichia coli the Min protein system plays an important role in positioning the division site. We show that this system also has an effect on timing of cell division. We do this in a quantitative way by measuring the cell division waiting time (defined as time difference between appearance of a division site and the division event) and the Z-ring existence time. Both quantities are found to be different in WT and cells without functional Min system. We develop a series of theoretical models whose predictions are compared with the experimental findings. Continuous improvement leads to a final model that is able to explain all relevant experimental observations. In particular, it shows that the chromosome segregation defect caused by the absence of Min proteins has an important influence on timing of cell division. Our results indicate that the Min system affects the septum formation rate. In the absence of the Min proteins this rate is reduced, leading to the observed strongly randomized cell division events and the longer division waiting times.Shuxin JiaDaniela KeilbergEdina HotMartin ThanbichlerLotte Søgaard-AndersenPeter LenzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103863 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuxin Jia Daniela Keilberg Edina Hot Martin Thanbichler Lotte Søgaard-Andersen Peter Lenz Effect of the Min system on timing of cell division in Escherichia coli.
description	In Escherichia coli the Min protein system plays an important role in positioning the division site. We show that this system also has an effect on timing of cell division. We do this in a quantitative way by measuring the cell division waiting time (defined as time difference between appearance of a division site and the division event) and the Z-ring existence time. Both quantities are found to be different in WT and cells without functional Min system. We develop a series of theoretical models whose predictions are compared with the experimental findings. Continuous improvement leads to a final model that is able to explain all relevant experimental observations. In particular, it shows that the chromosome segregation defect caused by the absence of Min proteins has an important influence on timing of cell division. Our results indicate that the Min system affects the septum formation rate. In the absence of the Min proteins this rate is reduced, leading to the observed strongly randomized cell division events and the longer division waiting times.
format	article
author	Shuxin Jia Daniela Keilberg Edina Hot Martin Thanbichler Lotte Søgaard-Andersen Peter Lenz
author_facet	Shuxin Jia Daniela Keilberg Edina Hot Martin Thanbichler Lotte Søgaard-Andersen Peter Lenz
author_sort	Shuxin Jia
title	Effect of the Min system on timing of cell division in Escherichia coli.
title_short	Effect of the Min system on timing of cell division in Escherichia coli.
title_full	Effect of the Min system on timing of cell division in Escherichia coli.
title_fullStr	Effect of the Min system on timing of cell division in Escherichia coli.
title_full_unstemmed	Effect of the Min system on timing of cell division in Escherichia coli.
title_sort	effect of the min system on timing of cell division in escherichia coli.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/18a8305ca809482884c7cc699e17137d
work_keys_str_mv	AT shuxinjia effectoftheminsystemontimingofcelldivisioninescherichiacoli AT danielakeilberg effectoftheminsystemontimingofcelldivisioninescherichiacoli AT edinahot effectoftheminsystemontimingofcelldivisioninescherichiacoli AT martinthanbichler effectoftheminsystemontimingofcelldivisioninescherichiacoli AT lottesøgaardandersen effectoftheminsystemontimingofcelldivisioninescherichiacoli AT peterlenz effectoftheminsystemontimingofcelldivisioninescherichiacoli
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Effect of the Min system on timing of cell division in Escherichia coli.

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