Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic Escherichia coli

Avian pathogenic Escherichia coli (APEC) is the responsible pathogen for colibacillosis in poultry, and is a potential gene source for human extraintestinal pathogenic Escherichia coli. Escherichia coli type III secretion system 2 (ETT2) is wi...

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Autores principales:	Jian Tu, Dandan Fu, Yi Gu, Ying Shao, Xiangjun Song, Mei Xue, Kezong Qi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	avian pathogenic <i>Escherichia coli</i> <i>Escherichia coli</i> type III secretion system 2 YgeK virulence regulation Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/18a959bbad0d4e18af9ebb11c2279de2
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spelling	oai:doaj.org-article:18a959bbad0d4e18af9ebb11c2279de22021-11-25T16:13:41ZTranscription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>10.3390/ani111130182076-2615https://doaj.org/article/18a959bbad0d4e18af9ebb11c2279de22021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3018https://doaj.org/toc/2076-2615Avian pathogenic <i>Escherichia coli</i> (APEC) is the responsible pathogen for colibacillosis in poultry, and is a potential gene source for human extraintestinal pathogenic <i>Escherichia coli</i>. <i>Escherichia coli</i> type III secretion system 2 (ETT2) is widely distributed in human and animal ExPEC isolates, and is crucial for the virulence of ExPEC. Transcriptional regulator YgeK, located in the ETT2 gene cluster, was identified as an important regulator of gene expression in enterohemorrhagic <i>E. coli</i> (EHEC). However, the role of YgeK in APEC has not been reported. In this study, we performed amino acid alignment analysis of YgeK among different <i>E. coli</i> strains and generated ygeK mutant strain AE81Δ<i>ygeK</i> from clinical APEC strain AE81. Flagellar formation, bacterial motility, serum sensitivity, adhesion, and virulence were all significantly reduced following the inactivation of YgeK in APEC. Then, we performed transcriptome sequencing to analyze the functional pathways involved in the biological processes. Results suggested that ETT2 transcriptional regulator YgeK plays a crucial role in APEC virulence. These findings thus contribute to our understanding of the function of the ETT2 cluster, and clarify the pathogenic mechanism of APEC.Jian TuDandan FuYi GuYing ShaoXiangjun SongMei XueKezong QiMDPI AGarticleavian pathogenic <i>Escherichia coli</i><i>Escherichia coli</i> type III secretion system 2YgeKvirulenceregulationVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3018, p 3018 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	avian pathogenic <i>Escherichia coli</i> <i>Escherichia coli</i> type III secretion system 2 YgeK virulence regulation Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	avian pathogenic <i>Escherichia coli</i> <i>Escherichia coli</i> type III secretion system 2 YgeK virulence regulation Veterinary medicine SF600-1100 Zoology QL1-991 Jian Tu Dandan Fu Yi Gu Ying Shao Xiangjun Song Mei Xue Kezong Qi Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
description	Avian pathogenic <i>Escherichia coli</i> (APEC) is the responsible pathogen for colibacillosis in poultry, and is a potential gene source for human extraintestinal pathogenic <i>Escherichia coli</i>. <i>Escherichia coli</i> type III secretion system 2 (ETT2) is widely distributed in human and animal ExPEC isolates, and is crucial for the virulence of ExPEC. Transcriptional regulator YgeK, located in the ETT2 gene cluster, was identified as an important regulator of gene expression in enterohemorrhagic <i>E. coli</i> (EHEC). However, the role of YgeK in APEC has not been reported. In this study, we performed amino acid alignment analysis of YgeK among different <i>E. coli</i> strains and generated ygeK mutant strain AE81Δ<i>ygeK</i> from clinical APEC strain AE81. Flagellar formation, bacterial motility, serum sensitivity, adhesion, and virulence were all significantly reduced following the inactivation of YgeK in APEC. Then, we performed transcriptome sequencing to analyze the functional pathways involved in the biological processes. Results suggested that ETT2 transcriptional regulator YgeK plays a crucial role in APEC virulence. These findings thus contribute to our understanding of the function of the ETT2 cluster, and clarify the pathogenic mechanism of APEC.
format	article
author	Jian Tu Dandan Fu Yi Gu Ying Shao Xiangjun Song Mei Xue Kezong Qi
author_facet	Jian Tu Dandan Fu Yi Gu Ying Shao Xiangjun Song Mei Xue Kezong Qi
author_sort	Jian Tu
title	Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
title_short	Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
title_full	Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
title_fullStr	Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
title_full_unstemmed	Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>
title_sort	transcription regulator ygek affects the virulence of avian pathogenic <i>escherichia coli</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/18a959bbad0d4e18af9ebb11c2279de2
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Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic <i>Escherichia coli</i>

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