OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH

Octamer transcription factor 1 (OCT1) is a transcriptional factor reported to be a poor prognostic factor in various cancers. However, the clinical value of OCT1 in breast cancer is not fully understood. In the present study, an immunohistochemical study of OCT1 protein was performed using estrogen...

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Autores principales:	Takuya Ogura, Kotaro Azuma, Junichiro Sato, Keiichi Kinowaki, Ken-Ichi Takayama, Toshihiko Takeiwa, Hidetaka Kawabata, Satoshi Inoue
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast cancer octamer transcription factor 1 (OCT1) non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) cell cycle proliferation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/18b003e97bbc4680a3ce167f7937deee
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spelling	oai:doaj.org-article:18b003e97bbc4680a3ce167f7937deee2021-11-11T16:58:01ZOCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH10.3390/ijms2221115051422-00671661-6596https://doaj.org/article/18b003e97bbc4680a3ce167f7937deee2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11505https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Octamer transcription factor 1 (OCT1) is a transcriptional factor reported to be a poor prognostic factor in various cancers. However, the clinical value of OCT1 in breast cancer is not fully understood. In the present study, an immunohistochemical study of OCT1 protein was performed using estrogen receptor (ER)-positive breast cancer tissues from 108 patients. Positive OCT1 immunoreactivity (IR) was associated with the shorter disease-free survival (DFS) of patients (<i>p</i> = 0.019). Knockdown of OCT1 inhibited cell proliferation in MCF-7 breast cancer cells as well as its derivative long-term estrogen-deprived (LTED) cells. On the other hand, the overexpression of OCT1 promoted cell proliferation in MCF-7 cells. Using microarray analysis, we identified the non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) as a novel OCT1-taget gene in MCF-7 cells. Immunohistochemical analysis showed that <i>NCAPH</i> IR was significantly positively associated with OCT1 IR (<i>p</i> < 0.001) and that positive <i>NCAPH</i> IR was significantly related to the poor DFS rate of patients (<i>p</i> = 0.041). The knockdown of <i>NCAPH</i> inhibited cell proliferation in MCF-7 and LTED cells. These results demonstrate that OCT1 and its target gene <i>NCAPH</i> are poor prognostic factors and potential therapeutic targets for patients with ER-positive breast cancer.Takuya OguraKotaro AzumaJunichiro SatoKeiichi KinowakiKen-Ichi TakayamaToshihiko TakeiwaHidetaka KawabataSatoshi InoueMDPI AGarticlebreast canceroctamer transcription factor 1 (OCT1)non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH)cell cycleproliferationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11505, p 11505 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer octamer transcription factor 1 (OCT1) non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) cell cycle proliferation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	breast cancer octamer transcription factor 1 (OCT1) non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) cell cycle proliferation Biology (General) QH301-705.5 Chemistry QD1-999 Takuya Ogura Kotaro Azuma Junichiro Sato Keiichi Kinowaki Ken-Ichi Takayama Toshihiko Takeiwa Hidetaka Kawabata Satoshi Inoue OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
description	Octamer transcription factor 1 (OCT1) is a transcriptional factor reported to be a poor prognostic factor in various cancers. However, the clinical value of OCT1 in breast cancer is not fully understood. In the present study, an immunohistochemical study of OCT1 protein was performed using estrogen receptor (ER)-positive breast cancer tissues from 108 patients. Positive OCT1 immunoreactivity (IR) was associated with the shorter disease-free survival (DFS) of patients (<i>p</i> = 0.019). Knockdown of OCT1 inhibited cell proliferation in MCF-7 breast cancer cells as well as its derivative long-term estrogen-deprived (LTED) cells. On the other hand, the overexpression of OCT1 promoted cell proliferation in MCF-7 cells. Using microarray analysis, we identified the non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) as a novel OCT1-taget gene in MCF-7 cells. Immunohistochemical analysis showed that <i>NCAPH</i> IR was significantly positively associated with OCT1 IR (<i>p</i> < 0.001) and that positive <i>NCAPH</i> IR was significantly related to the poor DFS rate of patients (<i>p</i> = 0.041). The knockdown of <i>NCAPH</i> inhibited cell proliferation in MCF-7 and LTED cells. These results demonstrate that OCT1 and its target gene <i>NCAPH</i> are poor prognostic factors and potential therapeutic targets for patients with ER-positive breast cancer.
format	article
author	Takuya Ogura Kotaro Azuma Junichiro Sato Keiichi Kinowaki Ken-Ichi Takayama Toshihiko Takeiwa Hidetaka Kawabata Satoshi Inoue
author_facet	Takuya Ogura Kotaro Azuma Junichiro Sato Keiichi Kinowaki Ken-Ichi Takayama Toshihiko Takeiwa Hidetaka Kawabata Satoshi Inoue
author_sort	Takuya Ogura
title	OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
title_short	OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
title_full	OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
title_fullStr	OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
title_full_unstemmed	OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH
title_sort	oct1 is a poor prognostic factor for breast cancer patients and promotes cell proliferation via inducing ncaph
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/18b003e97bbc4680a3ce167f7937deee
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OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH

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