Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.

Trypanosoma cruzi, the causative agent of Chagas disease, is a multiclonal parasite with high levels of genetic diversity and broad host and geographic ranges. Molecular characterization of South American isolates of T. cruzi has demonstrated homologous recombination and nuclear hybridization, as we...

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Autores principales:	Dawn M Roellig, Mason Y Savage, A Wendy Fujita, Christian Barnabé, Michel Tibayrenc, Frank J Steurer, Michael J Yabsley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18b32e26222b408895aa38fffdd90d59
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spelling	oai:doaj.org-article:18b32e26222b408895aa38fffdd90d592021-11-18T07:57:28ZGenetic variation and exchange in Trypanosoma cruzi isolates from the United States.1932-620310.1371/journal.pone.0056198https://doaj.org/article/18b32e26222b408895aa38fffdd90d592013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23457528/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Trypanosoma cruzi, the causative agent of Chagas disease, is a multiclonal parasite with high levels of genetic diversity and broad host and geographic ranges. Molecular characterization of South American isolates of T. cruzi has demonstrated homologous recombination and nuclear hybridization, as well as the presence of 6 main genetic clusters or "discrete typing units" (DTUs). Few studies have extensively investigated such exchange events and genetic diversity in North American isolates. In the current study, we genetically characterized over 50 US isolates from wildlife reservoirs (e.g., raccoons, opossums, armadillos, skunks), domestic dogs, humans, nonhuman primates, and reduviid vectors from nine states (TX, CA, OK, SC, FL, GA, MD, LA, TN) using a multilocus sequencing method. Single nucleotide polymorphisms were identified in sequences of the mismatch-repair class 2 (MSH2) and Tc52 genes. Typing based on the two genes often paralleled genotyping by classic methodologies using mini-exon and 18S and 24Sα rRNA genes. Evidence for genetic exchange was obtained by comparing sequence phylogenies of nuclear and mitochondrial gene targets, dihydrofolate reductase-thymidylate synthase (DHFR-TS) and the cytochrome oxidase subunit II- NADH dehydrogenase subunit I region (COII-ND1), respectively. We observed genetic exchange in several US isolates as demonstrated by incongruent mitochondrial and nuclear genes phylogenies, which confirms a previous finding of a single genetic exchange event in a Florida isolate. The presence of SNPs and evidence of genetic exchange illustrates that strains from the US are genetically diverse, even though only two phylogenetic lineages have been identified in this region.Dawn M RoelligMason Y SavageA Wendy FujitaChristian BarnabéMichel TibayrencFrank J SteurerMichael J YabsleyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56198 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dawn M Roellig Mason Y Savage A Wendy Fujita Christian Barnabé Michel Tibayrenc Frank J Steurer Michael J Yabsley Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
description	Trypanosoma cruzi, the causative agent of Chagas disease, is a multiclonal parasite with high levels of genetic diversity and broad host and geographic ranges. Molecular characterization of South American isolates of T. cruzi has demonstrated homologous recombination and nuclear hybridization, as well as the presence of 6 main genetic clusters or "discrete typing units" (DTUs). Few studies have extensively investigated such exchange events and genetic diversity in North American isolates. In the current study, we genetically characterized over 50 US isolates from wildlife reservoirs (e.g., raccoons, opossums, armadillos, skunks), domestic dogs, humans, nonhuman primates, and reduviid vectors from nine states (TX, CA, OK, SC, FL, GA, MD, LA, TN) using a multilocus sequencing method. Single nucleotide polymorphisms were identified in sequences of the mismatch-repair class 2 (MSH2) and Tc52 genes. Typing based on the two genes often paralleled genotyping by classic methodologies using mini-exon and 18S and 24Sα rRNA genes. Evidence for genetic exchange was obtained by comparing sequence phylogenies of nuclear and mitochondrial gene targets, dihydrofolate reductase-thymidylate synthase (DHFR-TS) and the cytochrome oxidase subunit II- NADH dehydrogenase subunit I region (COII-ND1), respectively. We observed genetic exchange in several US isolates as demonstrated by incongruent mitochondrial and nuclear genes phylogenies, which confirms a previous finding of a single genetic exchange event in a Florida isolate. The presence of SNPs and evidence of genetic exchange illustrates that strains from the US are genetically diverse, even though only two phylogenetic lineages have been identified in this region.
format	article
author	Dawn M Roellig Mason Y Savage A Wendy Fujita Christian Barnabé Michel Tibayrenc Frank J Steurer Michael J Yabsley
author_facet	Dawn M Roellig Mason Y Savage A Wendy Fujita Christian Barnabé Michel Tibayrenc Frank J Steurer Michael J Yabsley
author_sort	Dawn M Roellig
title	Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
title_short	Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
title_full	Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
title_fullStr	Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
title_full_unstemmed	Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.
title_sort	genetic variation and exchange in trypanosoma cruzi isolates from the united states.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/18b32e26222b408895aa38fffdd90d59
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Genetic variation and exchange in Trypanosoma cruzi isolates from the United States.

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