Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid

Abstract Polyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations o...

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Autores principales:	Yong-Mei Xing, Bing Li, Xu Zeng, Li-Si Zhou, Tae-Soo Lee, Min-Woong Lee, Xiao-Mei Chen, Shun-Xing Guo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18b354b6866d4154bb842b554f2633ab
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spelling	oai:doaj.org-article:18b354b6866d4154bb842b554f2633ab2021-12-02T16:38:49ZUse of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid10.1038/s41598-021-96740-72045-2322https://doaj.org/article/18b354b6866d4154bb842b554f2633ab2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96740-7https://doaj.org/toc/2045-2322Abstract Polyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations of oxalic acid. The differentially expressed genes (DEGs) encoding a series of oxidases were upregulated, and reductases were downregulated, in the low-oxalic-acid (Low OA) group compared to the control (No OA) group, while the opposite phenomenon was observed in the high-oxalic-acid (High OA) group. The detection of reactive oxygen species (ROS) in P. umbellatus mycelia was performed visually, and Ca2+ and H2O2 fluxes were measured using non-invasive micro-test technology (NMT). The sclerotial biomass in the Low OA group increased by 66%, however, no sclerotia formed in the High OA group. The ROS fluorescence intensity increased significantly in the Low OA group but decreased considerably in the High OA group. Ca2+ and H2O2 influx significantly increased in the Low OA group, while H2O2 exhibited efflux in the High OA group. A higher level of oxidative stress formed in the Low OA group. Different concentrations of oxalic acid were determined to affect P. umbellatus sclerotial formation in different ways.Yong-Mei XingBing LiXu ZengLi-Si ZhouTae-Soo LeeMin-Woong LeeXiao-Mei ChenShun-Xing GuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong-Mei Xing Bing Li Xu Zeng Li-Si Zhou Tae-Soo Lee Min-Woong Lee Xiao-Mei Chen Shun-Xing Guo Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
description	Abstract Polyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations of oxalic acid. The differentially expressed genes (DEGs) encoding a series of oxidases were upregulated, and reductases were downregulated, in the low-oxalic-acid (Low OA) group compared to the control (No OA) group, while the opposite phenomenon was observed in the high-oxalic-acid (High OA) group. The detection of reactive oxygen species (ROS) in P. umbellatus mycelia was performed visually, and Ca2+ and H2O2 fluxes were measured using non-invasive micro-test technology (NMT). The sclerotial biomass in the Low OA group increased by 66%, however, no sclerotia formed in the High OA group. The ROS fluorescence intensity increased significantly in the Low OA group but decreased considerably in the High OA group. Ca2+ and H2O2 influx significantly increased in the Low OA group, while H2O2 exhibited efflux in the High OA group. A higher level of oxidative stress formed in the Low OA group. Different concentrations of oxalic acid were determined to affect P. umbellatus sclerotial formation in different ways.
format	article
author	Yong-Mei Xing Bing Li Xu Zeng Li-Si Zhou Tae-Soo Lee Min-Woong Lee Xiao-Mei Chen Shun-Xing Guo
author_facet	Yong-Mei Xing Bing Li Xu Zeng Li-Si Zhou Tae-Soo Lee Min-Woong Lee Xiao-Mei Chen Shun-Xing Guo
author_sort	Yong-Mei Xing
title	Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
title_short	Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
title_full	Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
title_fullStr	Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
title_full_unstemmed	Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
title_sort	use of transcriptomic profiling to identify candidate genes involved in polyporus umbellatus sclerotial formation affected by oxalic acid
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/18b354b6866d4154bb842b554f2633ab
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Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid

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