Nicoletella semolina in the airways of healthy horses and horses with severe asthma

Abstract Background Nicoletella semolina was identified in the airways of horses and its low prevalence could be because of its difficult differentiation from other Pasteurellaceae. Objectives To develop a molecular method for the identification of N. semolina and to evaluate its prevalence in the m...

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Autores principales:	Flavie Payette, Audrey Charlebois, Julie‐Hélène Fairbrother, Guy Beauchamp, Mathilde Leclere
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	equine heaves microbiota Pasteurellaceae recurrent airway obstruction Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/18b36d3c0b6e43509a6f4122480e16f6
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spelling	oai:doaj.org-article:18b36d3c0b6e43509a6f4122480e16f62021-11-30T17:01:05ZNicoletella semolina in the airways of healthy horses and horses with severe asthma1939-16760891-664010.1111/jvim.16140https://doaj.org/article/18b36d3c0b6e43509a6f4122480e16f62021-05-01T00:00:00Zhttps://doi.org/10.1111/jvim.16140https://doaj.org/toc/0891-6640https://doaj.org/toc/1939-1676Abstract Background Nicoletella semolina was identified in the airways of horses and its low prevalence could be because of its difficult differentiation from other Pasteurellaceae. Objectives To develop a molecular method for the identification of N. semolina and to evaluate its prevalence in the mouth and the airways of healthy and severe asthmatic horses. Animals Six healthy and 6 severely asthmatic horses in phase I, 10 severely asthmatic horses in phase II, and 10 healthy horses in phase III. Methods Cohort (phases I and II) and cross‐sectional (phase III) studies. Quantitative polymerase chain reaction primers targeting the sodA gene were optimized. N. semolina was quantified in oral and nasal washes and in bronchoalveolar lavage fluid (BALF; phase I, sampled twice), in nasal washes and BALF (phase II, sampled twice), and in nasal washes (phase III). Results N. semolina was found in the nose of 5, 10, and 9 horses in phases I, II, and III, respectively (first sampling for phases I and II). Six BALF from 5 different horses were positive for N. semolina in phase II. In phase I, there was no significant difference in the nasal loads of healthy horses (median (range): 2.04 × 104 copies/mL (0‐2.44 × 105)) and asthmatic horses in exacerbation (3.75 × 102 (0‐4.84 × 106); Wilcoxon's rank sum test, P = .57). Conclusions and Clinical Importance N. semolina is commonly found in the airways of horses. The potential pathogenicity of N. semolina remains to be elucidated, but the molecular technique we developed will facilitate future studies.Flavie PayetteAudrey CharleboisJulie‐Hélène FairbrotherGuy BeauchampMathilde LeclereWileyarticleequineheavesmicrobiotaPasteurellaceaerecurrent airway obstructionVeterinary medicineSF600-1100ENJournal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1612-1619 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	equine heaves microbiota Pasteurellaceae recurrent airway obstruction Veterinary medicine SF600-1100
spellingShingle	equine heaves microbiota Pasteurellaceae recurrent airway obstruction Veterinary medicine SF600-1100 Flavie Payette Audrey Charlebois Julie‐Hélène Fairbrother Guy Beauchamp Mathilde Leclere Nicoletella semolina in the airways of healthy horses and horses with severe asthma
description	Abstract Background Nicoletella semolina was identified in the airways of horses and its low prevalence could be because of its difficult differentiation from other Pasteurellaceae. Objectives To develop a molecular method for the identification of N. semolina and to evaluate its prevalence in the mouth and the airways of healthy and severe asthmatic horses. Animals Six healthy and 6 severely asthmatic horses in phase I, 10 severely asthmatic horses in phase II, and 10 healthy horses in phase III. Methods Cohort (phases I and II) and cross‐sectional (phase III) studies. Quantitative polymerase chain reaction primers targeting the sodA gene were optimized. N. semolina was quantified in oral and nasal washes and in bronchoalveolar lavage fluid (BALF; phase I, sampled twice), in nasal washes and BALF (phase II, sampled twice), and in nasal washes (phase III). Results N. semolina was found in the nose of 5, 10, and 9 horses in phases I, II, and III, respectively (first sampling for phases I and II). Six BALF from 5 different horses were positive for N. semolina in phase II. In phase I, there was no significant difference in the nasal loads of healthy horses (median (range): 2.04 × 104 copies/mL (0‐2.44 × 105)) and asthmatic horses in exacerbation (3.75 × 102 (0‐4.84 × 106); Wilcoxon's rank sum test, P = .57). Conclusions and Clinical Importance N. semolina is commonly found in the airways of horses. The potential pathogenicity of N. semolina remains to be elucidated, but the molecular technique we developed will facilitate future studies.
format	article
author	Flavie Payette Audrey Charlebois Julie‐Hélène Fairbrother Guy Beauchamp Mathilde Leclere
author_facet	Flavie Payette Audrey Charlebois Julie‐Hélène Fairbrother Guy Beauchamp Mathilde Leclere
author_sort	Flavie Payette
title	Nicoletella semolina in the airways of healthy horses and horses with severe asthma
title_short	Nicoletella semolina in the airways of healthy horses and horses with severe asthma
title_full	Nicoletella semolina in the airways of healthy horses and horses with severe asthma
title_fullStr	Nicoletella semolina in the airways of healthy horses and horses with severe asthma
title_full_unstemmed	Nicoletella semolina in the airways of healthy horses and horses with severe asthma
title_sort	nicoletella semolina in the airways of healthy horses and horses with severe asthma
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/18b36d3c0b6e43509a6f4122480e16f6
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Nicoletella semolina in the airways of healthy horses and horses with severe asthma

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