Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture

Abstract Extracellular vesicles (EVs) are involved in intercellular communication and affect processes including immune and antiviral responses. Blood serum, a common cell culture medium component, is replete with EVs and must be depleted prior to EV-related experiments. The extent to which depletio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhaohao Liao, Dillon C. Muth, Erez Eitan, Meghan Travers, Lisa N. Learman, Elin Lehrmann, Kenneth W. Witwer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18c16c8f0c344963b09a8c194788588d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:18c16c8f0c344963b09a8c194788588d
record_format	dspace
spelling	oai:doaj.org-article:18c16c8f0c344963b09a8c194788588d2021-12-02T11:52:39ZSerum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture10.1038/s41598-017-02908-52045-2322https://doaj.org/article/18c16c8f0c344963b09a8c194788588d2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02908-5https://doaj.org/toc/2045-2322Abstract Extracellular vesicles (EVs) are involved in intercellular communication and affect processes including immune and antiviral responses. Blood serum, a common cell culture medium component, is replete with EVs and must be depleted prior to EV-related experiments. The extent to which depletion processes deplete non-EV particles is incompletely understood, but depleted serum is associated with reduced viability and growth in cell culture. Here, we examined whether serum depleted by two methods affected HIV-1 replication. In cell lines, including HIV-1 latency models, increased HIV-1 production was observed, along with changes in cell behavior and viability. Add-back of ultracentrifuge pellets (enriched in EVs but possibly other particles) rescued baseline HIV-1 production. Primary cells were less sensitive to serum depletion processes. Virus produced under processed serum conditions was more infectious. Finally, changes in cellular metabolism, surface markers, and gene expression, but not miRNA profiles, were associated with depleted serum culture. In conclusion, depleted serum conditions have a substantial effect on HIV-1 production and infectivity. Dependence of cell cultures on “whole serum” must be examined carefully along with other experimental variables, keeping in mind that the effects of EVs may be accompanied by or confused with those of closely associated or physically similar particles.Zhaohao LiaoDillon C. MuthErez EitanMeghan TraversLisa N. LearmanElin LehrmannKenneth W. WitwerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhaohao Liao Dillon C. Muth Erez Eitan Meghan Travers Lisa N. Learman Elin Lehrmann Kenneth W. Witwer Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
description	Abstract Extracellular vesicles (EVs) are involved in intercellular communication and affect processes including immune and antiviral responses. Blood serum, a common cell culture medium component, is replete with EVs and must be depleted prior to EV-related experiments. The extent to which depletion processes deplete non-EV particles is incompletely understood, but depleted serum is associated with reduced viability and growth in cell culture. Here, we examined whether serum depleted by two methods affected HIV-1 replication. In cell lines, including HIV-1 latency models, increased HIV-1 production was observed, along with changes in cell behavior and viability. Add-back of ultracentrifuge pellets (enriched in EVs but possibly other particles) rescued baseline HIV-1 production. Primary cells were less sensitive to serum depletion processes. Virus produced under processed serum conditions was more infectious. Finally, changes in cellular metabolism, surface markers, and gene expression, but not miRNA profiles, were associated with depleted serum culture. In conclusion, depleted serum conditions have a substantial effect on HIV-1 production and infectivity. Dependence of cell cultures on “whole serum” must be examined carefully along with other experimental variables, keeping in mind that the effects of EVs may be accompanied by or confused with those of closely associated or physically similar particles.
format	article
author	Zhaohao Liao Dillon C. Muth Erez Eitan Meghan Travers Lisa N. Learman Elin Lehrmann Kenneth W. Witwer
author_facet	Zhaohao Liao Dillon C. Muth Erez Eitan Meghan Travers Lisa N. Learman Elin Lehrmann Kenneth W. Witwer
author_sort	Zhaohao Liao
title	Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
title_short	Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
title_full	Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
title_fullStr	Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
title_full_unstemmed	Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
title_sort	serum extracellular vesicle depletion processes affect release and infectivity of hiv-1 in culture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/18c16c8f0c344963b09a8c194788588d
work_keys_str_mv	AT zhaohaoliao serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT dilloncmuth serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT erezeitan serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT meghantravers serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT lisanlearman serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT elinlehrmann serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture AT kennethwwitwer serumextracellularvesicledepletionprocessesaffectreleaseandinfectivityofhiv1inculture
_version_	1718394969169854464

Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture

Ejemplares similares