MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY

The microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology were studied. Materials and methods. 79 patients from GBUZ “Research Institute of Clinical Otorhinolaryngology” (78.5% patients with various forms of chronic tonsillitis and 21.5% of patients without chronic to...

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Autores principales:	O. Yu. Borisova, V. A. Aleshkin, A. S. Pimenova, A. I. Krukov, N. L. Kunelskaya, A. V. Gurov, G. B. Shadrin, A. S. Tovmasyan, B. A. Efimov, L. I. Kafarskaya
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	tonsillitis microflora microbiocenosis pharynx maldi-tof ms corynebacterium staphylococcus streptococcus candida albicans Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/18d1e39850614353ab320e3f455dd225
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spelling	oai:doaj.org-article:18d1e39850614353ab320e3f455dd2252021-11-22T07:09:47ZMICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY2220-76192313-739810.15789/2220-7619-2015-3-225-232https://doaj.org/article/18d1e39850614353ab320e3f455dd2252015-12-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/327https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology were studied. Materials and methods. 79 patients from GBUZ “Research Institute of Clinical Otorhinolaryngology” (78.5% patients with various forms of chronic tonsillitis and 21.5% of patients without chronic tonsillitis (control group). Among patients of the main group with chronic tonsillitis, at 60 (96.8%) patients there was a diagnosis chronic tonsillitis a toxical-allergic form of 1 degree (TAF I), at 1 (1.6%) the patient — chronic tonsillitis a toxical-allergic form II of degree (TAF II) and at 1 (1.6%) the patient — chronic tonsillitis. Identification of microorganisms carried out on cultural-morphological and biochemical properties. Specific identification of the hardly cultivated microorganisms and Corynebacterium was carried out by MALDI-TOFF MS of BioMerieux VITEK MS MALDI-TOF (“bioMerieux”, France). Identification of the allocated Corynebacterium was carried out by amplification of a gene rpoB and the subsequent direct sequencing. Results. The majority (98.7%) of the allocated microorganisms, treated 27 types and were Gram-positive. It is revealed 159 strains of 29 species of microorganisms, from them 41.4% of strains belonged to the Streptococcus, 19.7% — Staphylococcus, 36.9% — Corynebacterium. Among Streptococcus — 55.4% of S. viridans, 38.4% — S. pyogenes and 3.1% — of S. pneumoniae и S. oralis; Staphylococcus — 64.5% of S. aureus, 32.2% of S. epidermidis and 3.3% of S. hominis. 18 types of Corynebacterium — С. tuberculostearicum (17.2% strains), C. рseudodiphtheriticum (15.5% strains) and C. aurimucosum (18.9% strains) are revealed. At 44.3% of the surveyed patients the microflora is presented by a monoculture and at 55.7% associations are revealed. Conclusion. The microflora at patients with tonsillit’s pathology is characterized. At the expressed pathological process in a microbiota of a pharynx the monoculture while existence of associations testifies to less expressed pathological process prevails.O. Yu. BorisovaV. A. AleshkinA. S. PimenovaA. I. KrukovN. L. KunelskayaA. V. GurovG. B. ShadrinA. S. TovmasyanB. A. EfimovL. I. KafarskayaSankt-Peterburg : NIIÈM imeni Pasteraarticletonsillitismicrofloramicrobiocenosispharynxmaldi-tof mscorynebacteriumstaphylococcusstreptococcuscandida albicansInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 3, Pp 225-232 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	tonsillitis microflora microbiocenosis pharynx maldi-tof ms corynebacterium staphylococcus streptococcus candida albicans Infectious and parasitic diseases RC109-216
spellingShingle	tonsillitis microflora microbiocenosis pharynx maldi-tof ms corynebacterium staphylococcus streptococcus candida albicans Infectious and parasitic diseases RC109-216 O. Yu. Borisova V. A. Aleshkin A. S. Pimenova A. I. Krukov N. L. Kunelskaya A. V. Gurov G. B. Shadrin A. S. Tovmasyan B. A. Efimov L. I. Kafarskaya MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
description	The microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology were studied. Materials and methods. 79 patients from GBUZ “Research Institute of Clinical Otorhinolaryngology” (78.5% patients with various forms of chronic tonsillitis and 21.5% of patients without chronic tonsillitis (control group). Among patients of the main group with chronic tonsillitis, at 60 (96.8%) patients there was a diagnosis chronic tonsillitis a toxical-allergic form of 1 degree (TAF I), at 1 (1.6%) the patient — chronic tonsillitis a toxical-allergic form II of degree (TAF II) and at 1 (1.6%) the patient — chronic tonsillitis. Identification of microorganisms carried out on cultural-morphological and biochemical properties. Specific identification of the hardly cultivated microorganisms and Corynebacterium was carried out by MALDI-TOFF MS of BioMerieux VITEK MS MALDI-TOF (“bioMerieux”, France). Identification of the allocated Corynebacterium was carried out by amplification of a gene rpoB and the subsequent direct sequencing. Results. The majority (98.7%) of the allocated microorganisms, treated 27 types and were Gram-positive. It is revealed 159 strains of 29 species of microorganisms, from them 41.4% of strains belonged to the Streptococcus, 19.7% — Staphylococcus, 36.9% — Corynebacterium. Among Streptococcus — 55.4% of S. viridans, 38.4% — S. pyogenes and 3.1% — of S. pneumoniae и S. oralis; Staphylococcus — 64.5% of S. aureus, 32.2% of S. epidermidis and 3.3% of S. hominis. 18 types of Corynebacterium — С. tuberculostearicum (17.2% strains), C. рseudodiphtheriticum (15.5% strains) and C. aurimucosum (18.9% strains) are revealed. At 44.3% of the surveyed patients the microflora is presented by a monoculture and at 55.7% associations are revealed. Conclusion. The microflora at patients with tonsillit’s pathology is characterized. At the expressed pathological process in a microbiota of a pharynx the monoculture while existence of associations testifies to less expressed pathological process prevails.
format	article
author	O. Yu. Borisova V. A. Aleshkin A. S. Pimenova A. I. Krukov N. L. Kunelskaya A. V. Gurov G. B. Shadrin A. S. Tovmasyan B. A. Efimov L. I. Kafarskaya
author_facet	O. Yu. Borisova V. A. Aleshkin A. S. Pimenova A. I. Krukov N. L. Kunelskaya A. V. Gurov G. B. Shadrin A. S. Tovmasyan B. A. Efimov L. I. Kafarskaya
author_sort	O. Yu. Borisova
title	MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
title_short	MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
title_full	MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
title_fullStr	MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
title_full_unstemmed	MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY
title_sort	microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/18d1e39850614353ab320e3f455dd225
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MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY

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