Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers

Abstract. Arpiwi NL, Wahyuni IGAS, Muksin IK. 2019. Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers. Biodiversitas 20: 2134-2142. Pongamia pinnata (L.) Pierre is a member of family Leguminosae that produces seed oil for biodiesel feedstock. The aim...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ni Luh Arpiwi, I Gusti Ayu Sugi Wahyuni, I Ketut Muksin
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	pongamia pinnata, genetic diversity, issr, bali Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/18d746ba4a4645d6965a9de444b544b3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:18d746ba4a4645d6965a9de444b544b3
record_format	dspace
spelling	oai:doaj.org-article:18d746ba4a4645d6965a9de444b544b32021-11-21T21:43:55ZGenetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers1412-033X2085-472210.13057/biodiv/d200806https://doaj.org/article/18d746ba4a4645d6965a9de444b544b32019-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4027https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Arpiwi NL, Wahyuni IGAS, Muksin IK. 2019. Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers. Biodiversitas 20: 2134-2142. Pongamia pinnata (L.) Pierre is a member of family Leguminosae that produces seed oil for biodiesel feedstock. The aim of the present study was to determine genetic diversity of pongamia trees that grow in Bali using Inter Simple Sequence Repeat (ISSR) markers. This study is important to support the breeding program for the improvement of the biodiesel producing species. Leaf samples were taken from 26 pongamia trees grown on northern and southern coastal areas of Bali. Genomic DNA was isolated from fresh leaves sample and was amplified by Polymerase Chain Reaction (PCR) using 9 ISSR primers. The banding patterns of DNA after PCR were scored and tabulated into a binary matrix. Genetic distance was generated by pairwise distance using composite maximum likelihood. A dendrogram was constructed using Unweighted Pair Group Method Arithmetic (UPGMA) method. The binary matrix was further analyzed for Nonmetric Multidimensional Scaling (NMDS) with Primer E V.6 software. DNA concentrations ranged from 98.59-100.55 ng/?L with sufficient quality for PCR. The number of alleles for 9 primers was 43, the number of the polymorphic band was 35, and the number of monomorphic bands was 8. Percentage of polymorphism ranged from 50 to 100%. Cluster analysis of 26 DNA of pongamia trees showed that the trees were grouped into two, namely group I and II. Group I consisted of two trees only, namely Uma Anyar 1 and Penarukan 1. Group II consisted of 24 pongamia trees which were divided into 3 subgroups, namely IIA, IIB, and IIC with close genetic distance. Analysis of NMDS supported cluster analysis that 23 out of 26 pongamia trees had close genetic distance, and possibly they come from a similar source. Genetic diversity of pongamia in Bali needs to be widen possibly by the introduction of new planting materials from across Indonesia or seed procurement from different sources.Ni Luh ArpiwiI Gusti Ayu Sugi WahyuniI Ketut MuksinMBI & UNS Soloarticlepongamia pinnata, genetic diversity, issr, baliBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	pongamia pinnata, genetic diversity, issr, bali Biology (General) QH301-705.5
spellingShingle	pongamia pinnata, genetic diversity, issr, bali Biology (General) QH301-705.5 Ni Luh Arpiwi I Gusti Ayu Sugi Wahyuni I Ketut Muksin Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
description	Abstract. Arpiwi NL, Wahyuni IGAS, Muksin IK. 2019. Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers. Biodiversitas 20: 2134-2142. Pongamia pinnata (L.) Pierre is a member of family Leguminosae that produces seed oil for biodiesel feedstock. The aim of the present study was to determine genetic diversity of pongamia trees that grow in Bali using Inter Simple Sequence Repeat (ISSR) markers. This study is important to support the breeding program for the improvement of the biodiesel producing species. Leaf samples were taken from 26 pongamia trees grown on northern and southern coastal areas of Bali. Genomic DNA was isolated from fresh leaves sample and was amplified by Polymerase Chain Reaction (PCR) using 9 ISSR primers. The banding patterns of DNA after PCR were scored and tabulated into a binary matrix. Genetic distance was generated by pairwise distance using composite maximum likelihood. A dendrogram was constructed using Unweighted Pair Group Method Arithmetic (UPGMA) method. The binary matrix was further analyzed for Nonmetric Multidimensional Scaling (NMDS) with Primer E V.6 software. DNA concentrations ranged from 98.59-100.55 ng/?L with sufficient quality for PCR. The number of alleles for 9 primers was 43, the number of the polymorphic band was 35, and the number of monomorphic bands was 8. Percentage of polymorphism ranged from 50 to 100%. Cluster analysis of 26 DNA of pongamia trees showed that the trees were grouped into two, namely group I and II. Group I consisted of two trees only, namely Uma Anyar 1 and Penarukan 1. Group II consisted of 24 pongamia trees which were divided into 3 subgroups, namely IIA, IIB, and IIC with close genetic distance. Analysis of NMDS supported cluster analysis that 23 out of 26 pongamia trees had close genetic distance, and possibly they come from a similar source. Genetic diversity of pongamia in Bali needs to be widen possibly by the introduction of new planting materials from across Indonesia or seed procurement from different sources.
format	article
author	Ni Luh Arpiwi I Gusti Ayu Sugi Wahyuni I Ketut Muksin
author_facet	Ni Luh Arpiwi I Gusti Ayu Sugi Wahyuni I Ketut Muksin
author_sort	Ni Luh Arpiwi
title	Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
title_short	Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
title_full	Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
title_fullStr	Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
title_full_unstemmed	Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers
title_sort	genetic diversity of pongamia pinnata in bali, indonesia using inter simple sequence repeat markers
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/18d746ba4a4645d6965a9de444b544b3
work_keys_str_mv	AT niluharpiwi geneticdiversityofpongamiapinnatainbaliindonesiausingintersimplesequencerepeatmarkers AT igustiayusugiwahyuni geneticdiversityofpongamiapinnatainbaliindonesiausingintersimplesequencerepeatmarkers AT iketutmuksin geneticdiversityofpongamiapinnatainbaliindonesiausingintersimplesequencerepeatmarkers
_version_	1718418627470819328

Genetic diversity of Pongamia pinnata in Bali, Indonesia using Inter Simple Sequence Repeat markers

Ejemplares similares