A verified multiplexed immunoassay for detecting antibodies against infectious pathogens

Baseline screening of population groups at increased risk of developing infectious diseases (ID) includes a number of ELISA-based analyses, thereby accounting for high cost laborious and time-consuming examination. Earlier wedeveloped a cheap multiplex assay allowing to quickly perform a complex in...

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Autores principales:	A. G. Poltavchenko, O. V. Nechitaylo, P. V. Filatov, A. V. Ersh
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	infectious diseases antibodies initial complex testing multiplex dot-immunoassay sensitivity specificity quantitation Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/18d98363819a430a910a3610c6bb1f54
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spelling	oai:doaj.org-article:18d98363819a430a910a3610c6bb1f542021-11-22T07:09:52ZA verified multiplexed immunoassay for detecting antibodies against infectious pathogens2220-76192313-739810.15789/2220-7619-2019-1-209-215https://doaj.org/article/18d98363819a430a910a3610c6bb1f542019-05-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/614https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Baseline screening of population groups at increased risk of developing infectious diseases (ID) includes a number of ELISA-based analyses, thereby accounting for high cost laborious and time-consuming examination. Earlier wedeveloped a cheap multiplex assay allowing to quickly perform a complex initial testing. Here, we would like to describe a novel kit designed to simultaneously detect antibodies against the six ID pathogens in blood products (plasma, serum etc.): human immunodeficiency virus, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The kit is based on multiplex dot-immunoassay over flat protein arrays (immune chips) utilizing colloidal gold conjugates and silver development. It allows carrying out complex analysis at room temperature within 70 minutes and does not require highly-qualified personnel. In this article, the results of laboratory tests of the experimental sample of a multiplex kit are compared with commercial ELISA kits from five Russian manufacturers using an array of 240 blood product samples including 200 clinical serum (plasma) samples from patients with ID and 40 plasma samples from donors. It was shown that the multiplex test analysis has a sensitivity and specificity of at least 95%, and optical signals of the dot-assay correlate well with the optical density values obtained using commercial ELISA kits and allow a semiquantitative evaluation of the content of specific antibodies in the sample. Furthermore, multiplex analysis is quicker and cheaper compared to ELISA and can be carried out in field. The kit for multiplex dot-immunoassay of antibodies can provide a complex approach to the diagnosis of ID, significantly simplify initial testing, make it faster, more efficient and affordable for patients.A. G. PoltavchenkoO. V. NechitayloP. V. FilatovA. V. ErshSankt-Peterburg : NIIÈM imeni Pasteraarticleinfectious diseasesantibodiesinitial complex testingmultiplex dot-immunoassaysensitivityspecificityquantitationInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 1, Pp 209-215 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	infectious diseases antibodies initial complex testing multiplex dot-immunoassay sensitivity specificity quantitation Infectious and parasitic diseases RC109-216
spellingShingle	infectious diseases antibodies initial complex testing multiplex dot-immunoassay sensitivity specificity quantitation Infectious and parasitic diseases RC109-216 A. G. Poltavchenko O. V. Nechitaylo P. V. Filatov A. V. Ersh A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
description	Baseline screening of population groups at increased risk of developing infectious diseases (ID) includes a number of ELISA-based analyses, thereby accounting for high cost laborious and time-consuming examination. Earlier wedeveloped a cheap multiplex assay allowing to quickly perform a complex initial testing. Here, we would like to describe a novel kit designed to simultaneously detect antibodies against the six ID pathogens in blood products (plasma, serum etc.): human immunodeficiency virus, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The kit is based on multiplex dot-immunoassay over flat protein arrays (immune chips) utilizing colloidal gold conjugates and silver development. It allows carrying out complex analysis at room temperature within 70 minutes and does not require highly-qualified personnel. In this article, the results of laboratory tests of the experimental sample of a multiplex kit are compared with commercial ELISA kits from five Russian manufacturers using an array of 240 blood product samples including 200 clinical serum (plasma) samples from patients with ID and 40 plasma samples from donors. It was shown that the multiplex test analysis has a sensitivity and specificity of at least 95%, and optical signals of the dot-assay correlate well with the optical density values obtained using commercial ELISA kits and allow a semiquantitative evaluation of the content of specific antibodies in the sample. Furthermore, multiplex analysis is quicker and cheaper compared to ELISA and can be carried out in field. The kit for multiplex dot-immunoassay of antibodies can provide a complex approach to the diagnosis of ID, significantly simplify initial testing, make it faster, more efficient and affordable for patients.
format	article
author	A. G. Poltavchenko O. V. Nechitaylo P. V. Filatov A. V. Ersh
author_facet	A. G. Poltavchenko O. V. Nechitaylo P. V. Filatov A. V. Ersh
author_sort	A. G. Poltavchenko
title	A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
title_short	A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
title_full	A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
title_fullStr	A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
title_full_unstemmed	A verified multiplexed immunoassay for detecting antibodies against infectious pathogens
title_sort	verified multiplexed immunoassay for detecting antibodies against infectious pathogens
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/18d98363819a430a910a3610c6bb1f54
work_keys_str_mv	AT agpoltavchenko averifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT ovnechitaylo averifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT pvfilatov averifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT aversh averifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT agpoltavchenko verifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT ovnechitaylo verifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT pvfilatov verifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens AT aversh verifiedmultiplexedimmunoassayfordetectingantibodiesagainstinfectiouspathogens
_version_	1718417901966327808

A verified multiplexed immunoassay for detecting antibodies against infectious pathogens

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