BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION

Burn injuries are one of the key medical and social problems. Despite the significant achievements in combustiology, the slow healing and the appearance of infection are the key problems in burn patients, which lead to a longer hospitalization period, to reduction of life quality and to emotional di...

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Autores principales:	M. V. Osikov, A. A. Ageeva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	thermal trauma melatonin reparation cytokine tnfα ifnγ il-4 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/18e596691a6747939ca6e36952d522a7
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spelling	oai:doaj.org-article:18e596691a6747939ca6e36952d522a72021-11-18T08:03:51ZBLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION1563-06252313-741X10.15789/1563-0625-BCP-2214https://doaj.org/article/18e596691a6747939ca6e36952d522a72021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2419https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XBurn injuries are one of the key medical and social problems. Despite the significant achievements in combustiology, the slow healing and the appearance of infection are the key problems in burn patients, which lead to a longer hospitalization period, to reduction of life quality and to emotional disorders. Up to 70% of all complications after thermal trauma (TT) are connected with infection – first of all, pneumonia, infections of urinal tract. The forming of infectious complications, including sepsis, after TT is associated with excessive immunosuppressive reactions, as compensation for a long, stable proinflammatory response, in particular, owing to hyperproduction and effects of IL-10, IL-4, TGF-β. Aim: to study the influence of systemic and local usage of MT with original dermal film (DF) on reparation and serum cytokine concentration indicators in dynamics of experimental TT. The study was conducted using 84 rats – males of Wistar line, which were divided randomly into 4 groups: 1st group (n = 12) – intact monitoring, 2nd group (n = 30) – animals with TT, 3rd group (n = 21) – animals with TT and DF with MT use on the region of burn, 4th group (n = 21) – animals with TT and intraperitoneal injection of MT. To model TT of IIIA degree and relative area 3,5%, isolated skin area of interscapular area was immersed in distilled water at a temperature of 98-99 °С at 12 s. The DF with MT (at a concentration of 0.005 g/g) on 12 sm2 – area in 3rd group was used daily for 5 days. The MT was injected intraperitoneally daily at the dose of 10 mg/kg for 5 days. The wound area was calculated, the interleukin-4 (IL-4), tumor necrosis factor alpha (TNFα), interferon-gamma (IFNγ) were determined in serum on 5th,10th and 20th day from the moment of TT induction in each group. During experimental TT in dynamic monitoring from 5th to 20th day the absolute and relative areas of wound defect are reduced, because of that the epithelization speed and its part of area reduction are progressively increasing, on 5th,10th and 20th day the concentration of TNFα and IL-4 in serum is increasing with maximum values on 10th day of monitoring. Local usage of MT in DF during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th, 10th and 20th day. Intraperitoneal use of MT during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th and 20th day. The reparation accelerating effect of MT during TT is more expressed in locale usage in DF rather than using intraperitoneal injection.M. V. OsikovA. A. AgeevaSPb RAACIarticlethermal traumamelatoninreparationcytokinetnfαifnγil-4Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 705-710 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	thermal trauma melatonin reparation cytokine tnfα ifnγ il-4 Immunologic diseases. Allergy RC581-607
spellingShingle	thermal trauma melatonin reparation cytokine tnfα ifnγ il-4 Immunologic diseases. Allergy RC581-607 M. V. Osikov A. A. Ageeva BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
description	Burn injuries are one of the key medical and social problems. Despite the significant achievements in combustiology, the slow healing and the appearance of infection are the key problems in burn patients, which lead to a longer hospitalization period, to reduction of life quality and to emotional disorders. Up to 70% of all complications after thermal trauma (TT) are connected with infection – first of all, pneumonia, infections of urinal tract. The forming of infectious complications, including sepsis, after TT is associated with excessive immunosuppressive reactions, as compensation for a long, stable proinflammatory response, in particular, owing to hyperproduction and effects of IL-10, IL-4, TGF-β. Aim: to study the influence of systemic and local usage of MT with original dermal film (DF) on reparation and serum cytokine concentration indicators in dynamics of experimental TT. The study was conducted using 84 rats – males of Wistar line, which were divided randomly into 4 groups: 1st group (n = 12) – intact monitoring, 2nd group (n = 30) – animals with TT, 3rd group (n = 21) – animals with TT and DF with MT use on the region of burn, 4th group (n = 21) – animals with TT and intraperitoneal injection of MT. To model TT of IIIA degree and relative area 3,5%, isolated skin area of interscapular area was immersed in distilled water at a temperature of 98-99 °С at 12 s. The DF with MT (at a concentration of 0.005 g/g) on 12 sm2 – area in 3rd group was used daily for 5 days. The MT was injected intraperitoneally daily at the dose of 10 mg/kg for 5 days. The wound area was calculated, the interleukin-4 (IL-4), tumor necrosis factor alpha (TNFα), interferon-gamma (IFNγ) were determined in serum on 5th,10th and 20th day from the moment of TT induction in each group. During experimental TT in dynamic monitoring from 5th to 20th day the absolute and relative areas of wound defect are reduced, because of that the epithelization speed and its part of area reduction are progressively increasing, on 5th,10th and 20th day the concentration of TNFα and IL-4 in serum is increasing with maximum values on 10th day of monitoring. Local usage of MT in DF during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th, 10th and 20th day. Intraperitoneal use of MT during TT accelerates the healing of burn wound and lowers the TNFα and IL-4 concentration in serum on 5th and 20th day. The reparation accelerating effect of MT during TT is more expressed in locale usage in DF rather than using intraperitoneal injection.
format	article
author	M. V. Osikov A. A. Ageeva
author_facet	M. V. Osikov A. A. Ageeva
author_sort	M. V. Osikov
title	BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
title_short	BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
title_full	BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
title_fullStr	BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
title_full_unstemmed	BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION
title_sort	blood cytokine profile and lesion site repair in dynamics of experimental thermal trauma after local and systemic melatonin administration
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/18e596691a6747939ca6e36952d522a7
work_keys_str_mv	AT mvosikov bloodcytokineprofileandlesionsiterepairindynamicsofexperimentalthermaltraumaafterlocalandsystemicmelatoninadministration AT aaageeva bloodcytokineprofileandlesionsiterepairindynamicsofexperimentalthermaltraumaafterlocalandsystemicmelatoninadministration
_version_	1718422274092040192

BLOOD CYTOKINE PROFILE AND LESION SITE REPAIR IN DYNAMICS OF EXPERIMENTAL THERMAL TRAUMA AFTER LOCAL AND SYSTEMIC MELATONIN ADMINISTRATION

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