QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY

HIV infection poses a threat to the existence of humankind as a whole. Currently, lamivudine is widely used in antiretroviral therapy regimens. Modern methods of assessing quality, both in substance and in tablets, are characterized by a number of shortcomings: high cost of equipment, expensive GSO...

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Autores principales:	Y. A. Gonchikova, N. V. Chmelevskaya, E. A. Illarionova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	4,4'-диоксифталофенон lamivudine spесtrоphоtоmеtriс dеtеrminаtiоn phenolphtalein Science Q
Acceso en línea:	https://doaj.org/article/18e75546a6fc4e9396e2dda336a325c6
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spelling	oai:doaj.org-article:18e75546a6fc4e9396e2dda336a325c62021-11-23T06:14:38ZQUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY2541-94202587-959610.12737/article_59fad51c296659.55631274https://doaj.org/article/18e75546a6fc4e9396e2dda336a325c62017-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/419https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596HIV infection poses a threat to the existence of humankind as a whole. Currently, lamivudine is widely used in antiretroviral therapy regimens. Modern methods of assessing quality, both in substance and in tablets, are characterized by a number of shortcomings: high cost of equipment, expensive GSO production of the United States. The development of new methods for the analysis of lamivudine, as well as the improvement of existing methods, is an urgent problem. The purpose of this study is to develop a new technique for the quantitative determination of lamivudine by UV spectrophotometric method using a standard sample. The absorption spectrum of lamivudine solutions was studied at different pH values (1.1-14.1) in the wavelength range 200-400 nm. The optimal solvent for the spectrophotometric determination of the test substance was 0.1M HCl (pH 1.1). At pH 1.1, two absorption bands with absorption maxima at wavelengths of 212 ± 1 nm and 279 ± 1 nm are observed in the absorption spectrum of the lamivudine solution. A substance of organic nature, 4,4'-dihydroxyphthalophenone, was chosen as the standard sample for the determination of lamivudine. The maximum absorption of lamivudine (279 nm) is included in the optimal range for 4,4'-dihydroxy-phthalophenone (268-282 nm). The conversion ratio of lamivudine for 4,4'-dihydroxyphthalophenone is 0.1985. The error in determining lamivudine in the substance does not exceed 0.85 %, in tablets -1.6 %. The validation assessment confirmed the compliance of the developed methodology with normative documents and its suitability for analysis.Y. A. GonchikovaN. V. ChmelevskayaE. A. IllarionovaScientific Сentre for Family Health and Human Reproduction Problemsarticle4,4'-диоксифталофенонlamivudinespесtrоphоtоmеtriс dеtеrminаtiоnphenolphtaleinScienceQRUActa Biomedica Scientifica, Vol 2, Iss 4, Pp 90-92 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	4,4'-диоксифталофенон lamivudine spесtrоphоtоmеtriс dеtеrminаtiоn phenolphtalein Science Q
spellingShingle	4,4'-диоксифталофенон lamivudine spесtrоphоtоmеtriс dеtеrminаtiоn phenolphtalein Science Q Y. A. Gonchikova N. V. Chmelevskaya E. A. Illarionova QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
description	HIV infection poses a threat to the existence of humankind as a whole. Currently, lamivudine is widely used in antiretroviral therapy regimens. Modern methods of assessing quality, both in substance and in tablets, are characterized by a number of shortcomings: high cost of equipment, expensive GSO production of the United States. The development of new methods for the analysis of lamivudine, as well as the improvement of existing methods, is an urgent problem. The purpose of this study is to develop a new technique for the quantitative determination of lamivudine by UV spectrophotometric method using a standard sample. The absorption spectrum of lamivudine solutions was studied at different pH values (1.1-14.1) in the wavelength range 200-400 nm. The optimal solvent for the spectrophotometric determination of the test substance was 0.1M HCl (pH 1.1). At pH 1.1, two absorption bands with absorption maxima at wavelengths of 212 ± 1 nm and 279 ± 1 nm are observed in the absorption spectrum of the lamivudine solution. A substance of organic nature, 4,4'-dihydroxyphthalophenone, was chosen as the standard sample for the determination of lamivudine. The maximum absorption of lamivudine (279 nm) is included in the optimal range for 4,4'-dihydroxy-phthalophenone (268-282 nm). The conversion ratio of lamivudine for 4,4'-dihydroxyphthalophenone is 0.1985. The error in determining lamivudine in the substance does not exceed 0.85 %, in tablets -1.6 %. The validation assessment confirmed the compliance of the developed methodology with normative documents and its suitability for analysis.
format	article
author	Y. A. Gonchikova N. V. Chmelevskaya E. A. Illarionova
author_facet	Y. A. Gonchikova N. V. Chmelevskaya E. A. Illarionova
author_sort	Y. A. Gonchikova
title	QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
title_short	QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
title_full	QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
title_fullStr	QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
title_full_unstemmed	QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY
title_sort	quantitation of lamivudine by uv spectrophotometry
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/18e75546a6fc4e9396e2dda336a325c6
work_keys_str_mv	AT yagonchikova quantitationoflamivudinebyuvspectrophotometry AT nvchmelevskaya quantitationoflamivudinebyuvspectrophotometry AT eaillarionova quantitationoflamivudinebyuvspectrophotometry
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QUANTITATION OF LAMIVUDINE BY UV SPECTROPHOTOMETRY

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