Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.

<h4>Background</h4>Dendritic cells (DCs) are major modulators in the immune system. One active field of research is the manipulation of DCs as pharmacological targets to screen novel biological modifiers for the treatment of inflammatory and autoimmune disorders. Acetylcorynoline is the...

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Autores principales:	Ru-Huei Fu, Yu-Chi Wang, Shih-Ping Liu, Ching-Liang Chu, Rong-Tzong Tsai, Yu-Chen Ho, Wen-Lin Chang, Shao-Chih Chiu, Horng-Jyh Harn, Woei-Cherng Shyu, Shinn-Zong Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18f144219ccd4943b358a87fc1dfa34a
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spelling	oai:doaj.org-article:18f144219ccd4943b358a87fc1dfa34a2021-11-18T07:54:47ZAcetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.1932-620310.1371/journal.pone.0058398https://doaj.org/article/18f144219ccd4943b358a87fc1dfa34a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23472193/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dendritic cells (DCs) are major modulators in the immune system. One active field of research is the manipulation of DCs as pharmacological targets to screen novel biological modifiers for the treatment of inflammatory and autoimmune disorders. Acetylcorynoline is the major alkaloid component derived from Corydalis bungeana herbs. We assessed the capability of acetylcorynoline to regulate lipopolysaccharide (LPS)-stimulated activation of mouse bone marrow-derived DCs.<h4>Methodology/principal findings</h4>Our experimental data showed that treatment with up to 20 µM acetylcorynoline does not cause cytotoxicity in cells. Acetylcorynoline significantly inhibited the secretion of tumor necrosis factor-α, interleukin-6, and interleukin-12p70 by LPS-stimulated DCs. The expression of LPS-induced major histocompatibility complex class II, CD40, and CD86 on DCs was also decreased by acetylcorynoline, and the endocytic capacity of LPS-stimulated DCs was restored by acetylcorynoline. In addition, LPS-stimulated DC-elicited allogeneic T-cell proliferation was blocked by acetylcorynoline, and the migratory ability of LPS-stimulated DCs was reduced by acetylcorynoline. Moreover, acetylcorynoline significantly inhibits LPS-induced activation of IκB kinase and mitogen-activated protein kinase. Importantly, administration of acetylcorynoline significantly attenuates 2,4-dinitro-1-fluorobenzene-induced delayed-type hypersensitivity.<h4>Conclusions/significance</h4>Acetylcorynoline may be one of the potent immunosuppressive agents through the blockage of DC maturation and function.Ru-Huei FuYu-Chi WangShih-Ping LiuChing-Liang ChuRong-Tzong TsaiYu-Chen HoWen-Lin ChangShao-Chih ChiuHorng-Jyh HarnWoei-Cherng ShyuShinn-Zong LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58398 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ru-Huei Fu Yu-Chi Wang Shih-Ping Liu Ching-Liang Chu Rong-Tzong Tsai Yu-Chen Ho Wen-Lin Chang Shao-Chih Chiu Horng-Jyh Harn Woei-Cherng Shyu Shinn-Zong Lin Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
description	<h4>Background</h4>Dendritic cells (DCs) are major modulators in the immune system. One active field of research is the manipulation of DCs as pharmacological targets to screen novel biological modifiers for the treatment of inflammatory and autoimmune disorders. Acetylcorynoline is the major alkaloid component derived from Corydalis bungeana herbs. We assessed the capability of acetylcorynoline to regulate lipopolysaccharide (LPS)-stimulated activation of mouse bone marrow-derived DCs.<h4>Methodology/principal findings</h4>Our experimental data showed that treatment with up to 20 µM acetylcorynoline does not cause cytotoxicity in cells. Acetylcorynoline significantly inhibited the secretion of tumor necrosis factor-α, interleukin-6, and interleukin-12p70 by LPS-stimulated DCs. The expression of LPS-induced major histocompatibility complex class II, CD40, and CD86 on DCs was also decreased by acetylcorynoline, and the endocytic capacity of LPS-stimulated DCs was restored by acetylcorynoline. In addition, LPS-stimulated DC-elicited allogeneic T-cell proliferation was blocked by acetylcorynoline, and the migratory ability of LPS-stimulated DCs was reduced by acetylcorynoline. Moreover, acetylcorynoline significantly inhibits LPS-induced activation of IκB kinase and mitogen-activated protein kinase. Importantly, administration of acetylcorynoline significantly attenuates 2,4-dinitro-1-fluorobenzene-induced delayed-type hypersensitivity.<h4>Conclusions/significance</h4>Acetylcorynoline may be one of the potent immunosuppressive agents through the blockage of DC maturation and function.
format	article
author	Ru-Huei Fu Yu-Chi Wang Shih-Ping Liu Ching-Liang Chu Rong-Tzong Tsai Yu-Chen Ho Wen-Lin Chang Shao-Chih Chiu Horng-Jyh Harn Woei-Cherng Shyu Shinn-Zong Lin
author_facet	Ru-Huei Fu Yu-Chi Wang Shih-Ping Liu Ching-Liang Chu Rong-Tzong Tsai Yu-Chen Ho Wen-Lin Chang Shao-Chih Chiu Horng-Jyh Harn Woei-Cherng Shyu Shinn-Zong Lin
author_sort	Ru-Huei Fu
title	Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
title_short	Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
title_full	Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
title_fullStr	Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
title_full_unstemmed	Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.
title_sort	acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of iκb kinase and mitogen-activated protein kinase activities.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/18f144219ccd4943b358a87fc1dfa34a
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Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.

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