Arterial graft with elastic layer structure grown from cells

Abstract Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synt...

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Autores principales:	Utako Yokoyama, Yuta Tonooka, Ryoma Koretake, Taisuke Akimoto, Yuki Gonda, Junichi Saito, Masanari Umemura, Takayuki Fujita, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Yoshihiro Ishikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18f426ab4cb148cabc7ed7a370b160b4
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spelling	oai:doaj.org-article:18f426ab4cb148cabc7ed7a370b160b42021-12-02T16:06:46ZArterial graft with elastic layer structure grown from cells10.1038/s41598-017-00237-12045-2322https://doaj.org/article/18f426ab4cb148cabc7ed7a370b160b42017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00237-1https://doaj.org/toc/2045-2322Abstract Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. Here, we describe a method for fabrication of arterial grafts with elastic layer structure from cultured human vascular SMCs by periodic exposure to extremely high hydrostatic pressure (HP) during repeated cell seeding. Repeated slow cycles (0.002 Hz) between 110 and 180 kPa increased stress-fiber polymerization and fibronectin fibrillogenesis on SMCs, which is required for elastic fiber formation. To fabricate arterial grafts, seeding of rat vascular SMCs and exposure to the periodic HP were repeated alternatively ten times. The obtained medial grafts were highly elastic and tensile rupture strength was 1451 ± 159 mmHg, in which elastic fibers were abundantly formed. The patch medial grafts were sutured at the rat aorta and found to be completely patent and endothelialized after 2.5 months, although tubular medial constructs implanted in rats as interpositional aortic grafts withstood arterial blood pressure only in early acute phase. This novel organized self-assembly method would enable mass production of scaffold-free arterial grafts in vitro and have potential therapeutic applications for cardiovascular diseases.Utako YokoyamaYuta TonookaRyoma KoretakeTaisuke AkimotoYuki GondaJunichi SaitoMasanari UmemuraTakayuki FujitaShinya SakumaFumihito AraiMakoto KanekoYoshihiro IshikawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Utako Yokoyama Yuta Tonooka Ryoma Koretake Taisuke Akimoto Yuki Gonda Junichi Saito Masanari Umemura Takayuki Fujita Shinya Sakuma Fumihito Arai Makoto Kaneko Yoshihiro Ishikawa Arterial graft with elastic layer structure grown from cells
description	Abstract Shortage of autologous blood vessel sources and disadvantages of synthetic grafts have increased interest in the development of tissue-engineered vascular grafts. However, tunica media, which comprises layered elastic laminae, largely determines arterial elasticity, and is difficult to synthesize. Here, we describe a method for fabrication of arterial grafts with elastic layer structure from cultured human vascular SMCs by periodic exposure to extremely high hydrostatic pressure (HP) during repeated cell seeding. Repeated slow cycles (0.002 Hz) between 110 and 180 kPa increased stress-fiber polymerization and fibronectin fibrillogenesis on SMCs, which is required for elastic fiber formation. To fabricate arterial grafts, seeding of rat vascular SMCs and exposure to the periodic HP were repeated alternatively ten times. The obtained medial grafts were highly elastic and tensile rupture strength was 1451 ± 159 mmHg, in which elastic fibers were abundantly formed. The patch medial grafts were sutured at the rat aorta and found to be completely patent and endothelialized after 2.5 months, although tubular medial constructs implanted in rats as interpositional aortic grafts withstood arterial blood pressure only in early acute phase. This novel organized self-assembly method would enable mass production of scaffold-free arterial grafts in vitro and have potential therapeutic applications for cardiovascular diseases.
format	article
author	Utako Yokoyama Yuta Tonooka Ryoma Koretake Taisuke Akimoto Yuki Gonda Junichi Saito Masanari Umemura Takayuki Fujita Shinya Sakuma Fumihito Arai Makoto Kaneko Yoshihiro Ishikawa
author_facet	Utako Yokoyama Yuta Tonooka Ryoma Koretake Taisuke Akimoto Yuki Gonda Junichi Saito Masanari Umemura Takayuki Fujita Shinya Sakuma Fumihito Arai Makoto Kaneko Yoshihiro Ishikawa
author_sort	Utako Yokoyama
title	Arterial graft with elastic layer structure grown from cells
title_short	Arterial graft with elastic layer structure grown from cells
title_full	Arterial graft with elastic layer structure grown from cells
title_fullStr	Arterial graft with elastic layer structure grown from cells
title_full_unstemmed	Arterial graft with elastic layer structure grown from cells
title_sort	arterial graft with elastic layer structure grown from cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/18f426ab4cb148cabc7ed7a370b160b4
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_version_	1718384826709442560

Arterial graft with elastic layer structure grown from cells

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