Two major medicinal honeys have different mechanisms of bactericidal activity.

Honey is increasingly valued for its antibacterial activity, but knowledge regarding the mechanism of action is still incomplete. We assessed the bactericidal activity and mechanism of action of Revamil® source (RS) honey and manuka honey, the sources of two major medical-grade honeys. RS honey kill...

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Autores principales:	Paulus H S Kwakman, Anje A Te Velde, Leonie de Boer, Christina M J E Vandenbroucke-Grauls, Sebastian A J Zaat
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18ff9e01a5814d27bd41a415e1d539ff
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spelling	oai:doaj.org-article:18ff9e01a5814d27bd41a415e1d539ff2021-11-18T06:57:45ZTwo major medicinal honeys have different mechanisms of bactericidal activity.1932-620310.1371/journal.pone.0017709https://doaj.org/article/18ff9e01a5814d27bd41a415e1d539ff2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21394213/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Honey is increasingly valued for its antibacterial activity, but knowledge regarding the mechanism of action is still incomplete. We assessed the bactericidal activity and mechanism of action of Revamil® source (RS) honey and manuka honey, the sources of two major medical-grade honeys. RS honey killed Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa within 2 hours, whereas manuka honey had such rapid activity only against B. subtilis. After 24 hours of incubation, both honeys killed all tested bacteria, including methicillin-resistant Staphylococcus aureus, but manuka honey retained activity up to higher dilutions than RS honey. Bee defensin-1 and H₂O₂ were the major factors involved in rapid bactericidal activity of RS honey. These factors were absent in manuka honey, but this honey contained 44-fold higher concentrations of methylglyoxal than RS honey. Methylglyoxal was a major bactericidal factor in manuka honey, but after neutralization of this compound manuka honey retained bactericidal activity due to several unknown factors. RS and manuka honey have highly distinct compositions of bactericidal factors, resulting in large differences in bactericidal activity.Paulus H S KwakmanAnje A Te VeldeLeonie de BoerChristina M J E Vandenbroucke-GraulsSebastian A J ZaatPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17709 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paulus H S Kwakman Anje A Te Velde Leonie de Boer Christina M J E Vandenbroucke-Grauls Sebastian A J Zaat Two major medicinal honeys have different mechanisms of bactericidal activity.
description	Honey is increasingly valued for its antibacterial activity, but knowledge regarding the mechanism of action is still incomplete. We assessed the bactericidal activity and mechanism of action of Revamil® source (RS) honey and manuka honey, the sources of two major medical-grade honeys. RS honey killed Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa within 2 hours, whereas manuka honey had such rapid activity only against B. subtilis. After 24 hours of incubation, both honeys killed all tested bacteria, including methicillin-resistant Staphylococcus aureus, but manuka honey retained activity up to higher dilutions than RS honey. Bee defensin-1 and H₂O₂ were the major factors involved in rapid bactericidal activity of RS honey. These factors were absent in manuka honey, but this honey contained 44-fold higher concentrations of methylglyoxal than RS honey. Methylglyoxal was a major bactericidal factor in manuka honey, but after neutralization of this compound manuka honey retained bactericidal activity due to several unknown factors. RS and manuka honey have highly distinct compositions of bactericidal factors, resulting in large differences in bactericidal activity.
format	article
author	Paulus H S Kwakman Anje A Te Velde Leonie de Boer Christina M J E Vandenbroucke-Grauls Sebastian A J Zaat
author_facet	Paulus H S Kwakman Anje A Te Velde Leonie de Boer Christina M J E Vandenbroucke-Grauls Sebastian A J Zaat
author_sort	Paulus H S Kwakman
title	Two major medicinal honeys have different mechanisms of bactericidal activity.
title_short	Two major medicinal honeys have different mechanisms of bactericidal activity.
title_full	Two major medicinal honeys have different mechanisms of bactericidal activity.
title_fullStr	Two major medicinal honeys have different mechanisms of bactericidal activity.
title_full_unstemmed	Two major medicinal honeys have different mechanisms of bactericidal activity.
title_sort	two major medicinal honeys have different mechanisms of bactericidal activity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/18ff9e01a5814d27bd41a415e1d539ff
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Two major medicinal honeys have different mechanisms of bactericidal activity.

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