Novel protocol for persister cells isolation.

Bacterial persistence, where a fraction of a population presents a transient resistance to bactericidal substances, has great medical importance due to its relation with the appearance of antibiotic resistances and untreatable bacterial chronic infections. The mechanisms behind this phenomenon remai...

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Autores principales:	Silvia J Cañas-Duarte, Silvia Restrepo, Juan Manuel Pedraza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/18ffd7420738405a83a720635502dcd6
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spelling	oai:doaj.org-article:18ffd7420738405a83a720635502dcd62021-11-18T08:31:37ZNovel protocol for persister cells isolation.1932-620310.1371/journal.pone.0088660https://doaj.org/article/18ffd7420738405a83a720635502dcd62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586365/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial persistence, where a fraction of a population presents a transient resistance to bactericidal substances, has great medical importance due to its relation with the appearance of antibiotic resistances and untreatable bacterial chronic infections. The mechanisms behind this phenomenon remain largely unknown in spite of recent advances, in great part because of the difficulty in isolating the very small fraction of the population that is in this state at any given time. Current protocols for persister isolation have resulted in possible biases because of the induction of this state by the protocol itself. Here we present a novel protocol that allows rapid isolation of persister cells both from exponential and stationary phase. Moreover, it is capable of differentiating between type I and type II persister cells, which should allow the field to move beyond its current state of studying only one type. While this protocol prompts a revision of many of the current results, it should greatly facilitate further advances in the field.Silvia J Cañas-DuarteSilvia RestrepoJuan Manuel PedrazaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88660 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia J Cañas-Duarte Silvia Restrepo Juan Manuel Pedraza Novel protocol for persister cells isolation.
description	Bacterial persistence, where a fraction of a population presents a transient resistance to bactericidal substances, has great medical importance due to its relation with the appearance of antibiotic resistances and untreatable bacterial chronic infections. The mechanisms behind this phenomenon remain largely unknown in spite of recent advances, in great part because of the difficulty in isolating the very small fraction of the population that is in this state at any given time. Current protocols for persister isolation have resulted in possible biases because of the induction of this state by the protocol itself. Here we present a novel protocol that allows rapid isolation of persister cells both from exponential and stationary phase. Moreover, it is capable of differentiating between type I and type II persister cells, which should allow the field to move beyond its current state of studying only one type. While this protocol prompts a revision of many of the current results, it should greatly facilitate further advances in the field.
format	article
author	Silvia J Cañas-Duarte Silvia Restrepo Juan Manuel Pedraza
author_facet	Silvia J Cañas-Duarte Silvia Restrepo Juan Manuel Pedraza
author_sort	Silvia J Cañas-Duarte
title	Novel protocol for persister cells isolation.
title_short	Novel protocol for persister cells isolation.
title_full	Novel protocol for persister cells isolation.
title_fullStr	Novel protocol for persister cells isolation.
title_full_unstemmed	Novel protocol for persister cells isolation.
title_sort	novel protocol for persister cells isolation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/18ffd7420738405a83a720635502dcd6
work_keys_str_mv	AT silviajcanasduarte novelprotocolforpersistercellsisolation AT silviarestrepo novelprotocolforpersistercellsisolation AT juanmanuelpedraza novelprotocolforpersistercellsisolation
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Novel protocol for persister cells isolation.

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