Synapse clusters are preferentially formed by synapses with large recycling pool sizes.

Synapses are distributed heterogeneously in neural networks. The relationship between the spatial arrangement of synapses and an individual synapse's structural and functional features remains to be elucidated. Here, we examined the influence of the number of adjacent synapses on individual syn...

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Autores principales:	Oliver Welzel, Carsten H Tischbirek, Jasmin Jung, Eva M Kohler, Alexei Svetlitchny, Andreas W Henkel, Johannes Kornhuber, Teja W Groemer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1902516ba4a842ff8f9926545f740072
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spelling	oai:doaj.org-article:1902516ba4a842ff8f9926545f7400722021-11-18T07:03:05ZSynapse clusters are preferentially formed by synapses with large recycling pool sizes.1932-620310.1371/journal.pone.0013514https://doaj.org/article/1902516ba4a842ff8f9926545f7400722010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976002/?tool=EBIhttps://doaj.org/toc/1932-6203Synapses are distributed heterogeneously in neural networks. The relationship between the spatial arrangement of synapses and an individual synapse's structural and functional features remains to be elucidated. Here, we examined the influence of the number of adjacent synapses on individual synaptic recycling pool sizes. When measuring the discharge of the styryl dye FM1-43 from electrically stimulated synapses in rat hippocampal tissue cultures, a strong positive correlation between the number of neighbouring synapses and recycling vesicle pool sizes was observed. Accordingly, vesicle-rich synapses were found to preferentially reside next to neighbours with large recycling pool sizes. Although these synapses with large recycling pool sizes were rare, they were densely arranged and thus exhibited a high amount of release per volume. To consolidate these findings, functional terminals were marked by live-cell antibody staining with anti-synaptotagmin-1-cypHer or overexpression of synaptopHluorin. Analysis of synapse distributions in these systems confirmed the results obtained with FM 1-43. Our findings support the idea that clustering of synapses with large recycling pool sizes is a distinct developmental feature of newly formed neural networks and may contribute to functional plasticity.Oliver WelzelCarsten H TischbirekJasmin JungEva M KohlerAlexei SvetlitchnyAndreas W HenkelJohannes KornhuberTeja W GroemerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13514 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliver Welzel Carsten H Tischbirek Jasmin Jung Eva M Kohler Alexei Svetlitchny Andreas W Henkel Johannes Kornhuber Teja W Groemer Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
description	Synapses are distributed heterogeneously in neural networks. The relationship between the spatial arrangement of synapses and an individual synapse's structural and functional features remains to be elucidated. Here, we examined the influence of the number of adjacent synapses on individual synaptic recycling pool sizes. When measuring the discharge of the styryl dye FM1-43 from electrically stimulated synapses in rat hippocampal tissue cultures, a strong positive correlation between the number of neighbouring synapses and recycling vesicle pool sizes was observed. Accordingly, vesicle-rich synapses were found to preferentially reside next to neighbours with large recycling pool sizes. Although these synapses with large recycling pool sizes were rare, they were densely arranged and thus exhibited a high amount of release per volume. To consolidate these findings, functional terminals were marked by live-cell antibody staining with anti-synaptotagmin-1-cypHer or overexpression of synaptopHluorin. Analysis of synapse distributions in these systems confirmed the results obtained with FM 1-43. Our findings support the idea that clustering of synapses with large recycling pool sizes is a distinct developmental feature of newly formed neural networks and may contribute to functional plasticity.
format	article
author	Oliver Welzel Carsten H Tischbirek Jasmin Jung Eva M Kohler Alexei Svetlitchny Andreas W Henkel Johannes Kornhuber Teja W Groemer
author_facet	Oliver Welzel Carsten H Tischbirek Jasmin Jung Eva M Kohler Alexei Svetlitchny Andreas W Henkel Johannes Kornhuber Teja W Groemer
author_sort	Oliver Welzel
title	Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
title_short	Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
title_full	Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
title_fullStr	Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
title_full_unstemmed	Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
title_sort	synapse clusters are preferentially formed by synapses with large recycling pool sizes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/1902516ba4a842ff8f9926545f740072
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Synapse clusters are preferentially formed by synapses with large recycling pool sizes.

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