A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.
Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the ges...
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Public Library of Science (PLoS)
2014
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oai:doaj.org-article:1909cfc02a3c4c76a5ebc22e35d21d002021-11-18T08:16:48ZA gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.1932-620310.1371/journal.pone.0098667https://doaj.org/article/1909cfc02a3c4c76a5ebc22e35d21d002014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24905832/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the gestational-age release profile and bioactivity of placental cell-derived exosome in maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant and pregnant women in the first (FT, 6-12 weeks), second (ST, 22-24 weeks) and third (TT, 32-38 weeks) trimester. The number of exosomes and placental exosome contribution were determined by quantifying immunoreactive exosomal CD63 and placenta-specific marker (PLAP), respectively. The effect of exosomes isolated from FT, ST and TT on endothelial cell migration were established using a real-time, live-cell imaging system (Incucyte). Exosome plasma concentration was more than 50-fold greater in pregnant women than in non-pregnant women (p<0.001). During normal healthy pregnancy, the number of exosomes present in maternal plasma increased significantly with gestational age by more that two-fold (p<0.001). Exosomes isolated from FT, ST and TT increased endothelial cell migration by 1.9±0.1, 1.6±0.2 and 1.3±0.1-fold, respectively compared to the control. Pregnancy is associated with a dramatic increase in the number of exosomes present in plasma and maternal plasma exosomes are bioactive. While the role of placental cell-derived exosome in regulating maternal and/or fetal vascular responses remains to be elucidated, changes in exosome profile may be of clinical utility in the diagnosis of placental dysfunction.Carlos SalomonMaria Jose TorresMiharu KobayashiKatherin Scholz-RomeroLuis SobreviaAneta DobierzewskaSebastian E IllanesMurray D MitchellGregory E RicePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98667 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| description |
Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the gestational-age release profile and bioactivity of placental cell-derived exosome in maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant and pregnant women in the first (FT, 6-12 weeks), second (ST, 22-24 weeks) and third (TT, 32-38 weeks) trimester. The number of exosomes and placental exosome contribution were determined by quantifying immunoreactive exosomal CD63 and placenta-specific marker (PLAP), respectively. The effect of exosomes isolated from FT, ST and TT on endothelial cell migration were established using a real-time, live-cell imaging system (Incucyte). Exosome plasma concentration was more than 50-fold greater in pregnant women than in non-pregnant women (p<0.001). During normal healthy pregnancy, the number of exosomes present in maternal plasma increased significantly with gestational age by more that two-fold (p<0.001). Exosomes isolated from FT, ST and TT increased endothelial cell migration by 1.9±0.1, 1.6±0.2 and 1.3±0.1-fold, respectively compared to the control. Pregnancy is associated with a dramatic increase in the number of exosomes present in plasma and maternal plasma exosomes are bioactive. While the role of placental cell-derived exosome in regulating maternal and/or fetal vascular responses remains to be elucidated, changes in exosome profile may be of clinical utility in the diagnosis of placental dysfunction. |
| format |
article |
| author |
Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice |
| author_facet |
Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice |
| author_sort |
Carlos Salomon |
| title |
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_short |
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_full |
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_fullStr |
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_full_unstemmed |
A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_sort |
gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/1909cfc02a3c4c76a5ebc22e35d21d00 |
| work_keys_str_mv |
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1718421969097981952 |