TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients

Birhanu Ayelign,1 Meaza Genetu,1 Tadelo Wondmagegn,1 Gashaw Adane,1 Markos Negash,1 Nega Berhane2 1Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopa; 2Institute of Biotechnology,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ayelign B, Genetu M, Wondmagegn T, Adane G, Negash M, Berhane N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	tnf-α t2dm and north west ethiopia Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/190e99fd1d734d1cb3cf6ce718b1083e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:190e99fd1d734d1cb3cf6ce718b1083e
record_format	dspace
spelling	oai:doaj.org-article:190e99fd1d734d1cb3cf6ce718b1083e2021-12-02T05:07:33ZTNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients1178-7007https://doaj.org/article/190e99fd1d734d1cb3cf6ce718b1083e2019-11-01T00:00:00Zhttps://www.dovepress.com/tnf-alpha-minus308-gene-polymorphism-and-type-2-diabetes-mellitus-in-e-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Birhanu Ayelign,1 Meaza Genetu,1 Tadelo Wondmagegn,1 Gashaw Adane,1 Markos Negash,1 Nega Berhane2 1Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopa; 2Institute of Biotechnology, College of Computational and Natural Science, University of Gondar, Gondar, EthiopaCorrespondence: Birhanu AyelignDepartment of Immunology and Molecular Biology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, EthiopaEmail birhanuayelign42@gmail.comBackground: Type 2 diabetes mellitus (T2DM) is a metabolic disorder resulting from insulin insufficiency or function. Predisposing factors for T2DM are mainly genetic and environmental. Genetic polymorphism of cytokines like tumor necrosis factor-alpha (TNF-α) is suggestive of interference with insulin-sensitive glucose uptake and induces insulin resistance that ultimately could lead to T2DM. In this study, we assessed the effect of TNF-α (−308) G/A gene polymorphism and its association with the development of T2DM in an Ethiopian population.Methods: An institutional-based cross-sectional study was conducted on study subjects with T2DM and non-diabetic healthy controls. DNA was extracted and genotyping was carried out by using amplification refractory mutation system polymerase chain reaction. A genetic-polymorphism on TNF-α (−308) G/A with T2DM was evaluated by logistic regression and Student’s t-test. A P-value Results: In the present study, we observed a significant association between T2DM and TNF-α (−308) gene polymorphism’s GG genotype [χ2 test P = 0.005, OR (95% CI) =2.667 (1.309–5.45d8)]. In contrast, no statistically significant differences were observed in the frequencies of genotypes AA and AG (χ2 test P=0.132 and 0.067, respectively). Moreover, T2DM individuals had higher concentrations of lipid profiles for those carrying the TNF-α (−308) GG genotype as compared to the control group.Conclusion: TNF-α (−308) genetic polymorphism may be implicated in the genetic susceptibility for, as well as the development of T2DM and lipid metabolism in the Ethiopian population. Therefore, a large-scale study and early screening of TNF-α (−308) genetic polymorphism may help in early management and control of diabetes and related outcomes.Keywords: TNF-α, T2DM, North West EthiopiaAyelign BGenetu MWondmagegn TAdane GNegash MBerhane NDove Medical Pressarticletnf-αt2dmand north west ethiopiaSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2453-2459 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	tnf-α t2dm and north west ethiopia Specialties of internal medicine RC581-951
spellingShingle	tnf-α t2dm and north west ethiopia Specialties of internal medicine RC581-951 Ayelign B Genetu M Wondmagegn T Adane G Negash M Berhane N TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
description	Birhanu Ayelign,1 Meaza Genetu,1 Tadelo Wondmagegn,1 Gashaw Adane,1 Markos Negash,1 Nega Berhane2 1Department of Immunology and Molecular Biology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopa; 2Institute of Biotechnology, College of Computational and Natural Science, University of Gondar, Gondar, EthiopaCorrespondence: Birhanu AyelignDepartment of Immunology and Molecular Biology, School of Biomedical and Laboratory Science, College of Medicine and Health Science, University of Gondar, Gondar, EthiopaEmail birhanuayelign42@gmail.comBackground: Type 2 diabetes mellitus (T2DM) is a metabolic disorder resulting from insulin insufficiency or function. Predisposing factors for T2DM are mainly genetic and environmental. Genetic polymorphism of cytokines like tumor necrosis factor-alpha (TNF-α) is suggestive of interference with insulin-sensitive glucose uptake and induces insulin resistance that ultimately could lead to T2DM. In this study, we assessed the effect of TNF-α (−308) G/A gene polymorphism and its association with the development of T2DM in an Ethiopian population.Methods: An institutional-based cross-sectional study was conducted on study subjects with T2DM and non-diabetic healthy controls. DNA was extracted and genotyping was carried out by using amplification refractory mutation system polymerase chain reaction. A genetic-polymorphism on TNF-α (−308) G/A with T2DM was evaluated by logistic regression and Student’s t-test. A P-value Results: In the present study, we observed a significant association between T2DM and TNF-α (−308) gene polymorphism’s GG genotype [χ2 test P = 0.005, OR (95% CI) =2.667 (1.309–5.45d8)]. In contrast, no statistically significant differences were observed in the frequencies of genotypes AA and AG (χ2 test P=0.132 and 0.067, respectively). Moreover, T2DM individuals had higher concentrations of lipid profiles for those carrying the TNF-α (−308) GG genotype as compared to the control group.Conclusion: TNF-α (−308) genetic polymorphism may be implicated in the genetic susceptibility for, as well as the development of T2DM and lipid metabolism in the Ethiopian population. Therefore, a large-scale study and early screening of TNF-α (−308) genetic polymorphism may help in early management and control of diabetes and related outcomes.Keywords: TNF-α, T2DM, North West Ethiopia
format	article
author	Ayelign B Genetu M Wondmagegn T Adane G Negash M Berhane N
author_facet	Ayelign B Genetu M Wondmagegn T Adane G Negash M Berhane N
author_sort	Ayelign B
title	TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
title_short	TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
title_full	TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
title_fullStr	TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
title_full_unstemmed	TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients
title_sort	tnf-α (−308) gene polymorphism and type 2 diabetes mellitus in ethiopian diabetes patients
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/190e99fd1d734d1cb3cf6ce718b1083e
work_keys_str_mv	AT ayelignb tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients AT genetum tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients AT wondmagegnt tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients AT adaneg tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients AT negashm tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients AT berhanen tnfa308genepolymorphismandtype2diabetesmellitusinethiopiandiabetespatients
_version_	1718400574641143808

TNF-α (−308) Gene Polymorphism and Type 2 Diabetes Mellitus in Ethiopian Diabetes Patients

Ejemplares similares