Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo

Abstract Slow wave activity (SWA) is a characteristic brain oscillation in sleep and quiet wakefulness. Although the cell types contributing to SWA genesis are not yet identified, the principal role of neurons in the emergence of this essential cognitive mechanism has not been questioned. To address...

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Autores principales:	Zsolt Szabó, László Héja, Gergely Szalay, Orsolya Kékesi, András Füredi, Kornélia Szebényi, Árpád Dobolyi, Tamás I. Orbán, Orsolya Kolacsek, Tamás Tompa, Zsombor Miskolczy, László Biczók, Balázs Rózsa, Balázs Sarkadi, Julianna Kardos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1921ccf7c5df4463833ab0d18cec794a
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spelling	oai:doaj.org-article:1921ccf7c5df4463833ab0d18cec794a2021-12-02T11:52:15ZExtensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo10.1038/s41598-017-06073-72045-2322https://doaj.org/article/1921ccf7c5df4463833ab0d18cec794a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06073-7https://doaj.org/toc/2045-2322Abstract Slow wave activity (SWA) is a characteristic brain oscillation in sleep and quiet wakefulness. Although the cell types contributing to SWA genesis are not yet identified, the principal role of neurons in the emergence of this essential cognitive mechanism has not been questioned. To address the possibility of astrocytic involvement in SWA, we used a transgenic rat line expressing a calcium sensitive fluorescent protein in both astrocytes and interneurons and simultaneously imaged astrocytic and neuronal activity in vivo. Here we demonstrate, for the first time, that the astrocyte network display synchronized recurrent activity in vivo coupled to UP states measured by field recording and neuronal calcium imaging. Furthermore, we present evidence that extensive synchronization of the astrocytic network precedes the spatial build-up of neuronal synchronization. The earlier extensive recruitment of astrocytes in the synchronized activity is reinforced by the observation that neurons surrounded by active astrocytes are more likely to join SWA, suggesting causality. Further supporting this notion, we demonstrate that blockade of astrocytic gap junctional communication or inhibition of astrocytic Ca2+ transients reduces the ratio of both astrocytes and neurons involved in SWA. These in vivo findings conclusively suggest a causal role of the astrocytic syncytium in SWA generation.Zsolt SzabóLászló HéjaGergely SzalayOrsolya KékesiAndrás FürediKornélia SzebényiÁrpád DobolyiTamás I. OrbánOrsolya KolacsekTamás TompaZsombor MiskolczyLászló BiczókBalázs RózsaBalázs SarkadiJulianna KardosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zsolt Szabó László Héja Gergely Szalay Orsolya Kékesi András Füredi Kornélia Szebényi Árpád Dobolyi Tamás I. Orbán Orsolya Kolacsek Tamás Tompa Zsombor Miskolczy László Biczók Balázs Rózsa Balázs Sarkadi Julianna Kardos Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
description	Abstract Slow wave activity (SWA) is a characteristic brain oscillation in sleep and quiet wakefulness. Although the cell types contributing to SWA genesis are not yet identified, the principal role of neurons in the emergence of this essential cognitive mechanism has not been questioned. To address the possibility of astrocytic involvement in SWA, we used a transgenic rat line expressing a calcium sensitive fluorescent protein in both astrocytes and interneurons and simultaneously imaged astrocytic and neuronal activity in vivo. Here we demonstrate, for the first time, that the astrocyte network display synchronized recurrent activity in vivo coupled to UP states measured by field recording and neuronal calcium imaging. Furthermore, we present evidence that extensive synchronization of the astrocytic network precedes the spatial build-up of neuronal synchronization. The earlier extensive recruitment of astrocytes in the synchronized activity is reinforced by the observation that neurons surrounded by active astrocytes are more likely to join SWA, suggesting causality. Further supporting this notion, we demonstrate that blockade of astrocytic gap junctional communication or inhibition of astrocytic Ca2+ transients reduces the ratio of both astrocytes and neurons involved in SWA. These in vivo findings conclusively suggest a causal role of the astrocytic syncytium in SWA generation.
format	article
author	Zsolt Szabó László Héja Gergely Szalay Orsolya Kékesi András Füredi Kornélia Szebényi Árpád Dobolyi Tamás I. Orbán Orsolya Kolacsek Tamás Tompa Zsombor Miskolczy László Biczók Balázs Rózsa Balázs Sarkadi Julianna Kardos
author_facet	Zsolt Szabó László Héja Gergely Szalay Orsolya Kékesi András Füredi Kornélia Szebényi Árpád Dobolyi Tamás I. Orbán Orsolya Kolacsek Tamás Tompa Zsombor Miskolczy László Biczók Balázs Rózsa Balázs Sarkadi Julianna Kardos
author_sort	Zsolt Szabó
title	Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
title_short	Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
title_full	Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
title_fullStr	Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
title_full_unstemmed	Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
title_sort	extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1921ccf7c5df4463833ab0d18cec794a
work_keys_str_mv	AT zsoltszabo extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT laszloheja extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT gergelyszalay extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT orsolyakekesi extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT andrasfuredi extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT korneliaszebenyi extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT arpaddobolyi extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT tamasiorban extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT orsolyakolacsek extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT tamastompa extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT zsombormiskolczy extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT laszlobiczok extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT balazsrozsa extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT balazssarkadi extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo AT juliannakardos extensiveastrocytesynchronizationadvancesneuronalcouplinginslowwaveactivityinvivo
_version_	1718395089880875008

Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo

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