Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story

The lesser Antilles archipelago in the Caribbean is known as a biodiversity hotspot, hosting many endemic species. However, recent introduction of a highly invasive species, the Australian redclaw crayfish (Cherax quadricarinatus), has led to significant threats to this fragile ecosystem. Here we de...

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Autores principales:	Thomas Baudry, Quentin Mauvisseau, Jean-Pierre Goût, Alexandre Arqué, Carine Delaunay, Juliette Smith-Ravin, Michael Sweet, Frédéric Grandjean
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biodiversity hotspot Cherax quadricarinatus Environmental DNA Freshwater Invasive species qPCR Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/19258642cb854b8b981b27e09daa242e
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id	oai:doaj.org-article:19258642cb854b8b981b27e09daa242e
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spelling	oai:doaj.org-article:19258642cb854b8b981b27e09daa242e2021-12-01T04:50:04ZMapping a super-invader in a biodiversity hotspot, an eDNA-based success story1470-160X10.1016/j.ecolind.2021.107637https://doaj.org/article/19258642cb854b8b981b27e09daa242e2021-07-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21003022https://doaj.org/toc/1470-160XThe lesser Antilles archipelago in the Caribbean is known as a biodiversity hotspot, hosting many endemic species. However, recent introduction of a highly invasive species, the Australian redclaw crayfish (Cherax quadricarinatus), has led to significant threats to this fragile ecosystem. Here we developed, validated, and optimized a species-specific eDNA-based detection protocol targeting the 16S region of the mitochondrial gene of C. quadricarinatus. Our aim was to assess the crayfish distribution across Martinique Island. Our developed assay was species-specific and showed high sensitivity in laboratory, mesocosm and field conditions. A significant and positive correlation was found between species biomass, detection probability and efficiency through mesocosm experiments. Moreover, we found eDNA persisted up to 23 days in tropical freshwaters. We investigated a total of 83 locations, spread over 53 rivers and two closed water basins using our novel eDNA assay and traditional trapping, the latter, undertaken to confirm the reliability of the molecular-based detection method. Overall, we detected C. quadricarinatus at 47 locations using eDNA and 28 using traditional trapping, all positive trapping sites were positive for eDNA. We found that eDNA-based monitoring was less time-consuming and less influenced by the crayfishes often patchy distributions, proving a more reliable tool for future large-scale surveys. The clear threat and worrying distribution of this invasive species is particularly alarming as the archipelago belongs to one of the 25 identified biodiversity hotspots on Earth.Thomas BaudryQuentin MauvisseauJean-Pierre GoûtAlexandre ArquéCarine DelaunayJuliette Smith-RavinMichael SweetFrédéric GrandjeanElsevierarticleBiodiversity hotspotCherax quadricarinatusEnvironmental DNAFreshwaterInvasive speciesqPCREcologyQH540-549.5ENEcological Indicators, Vol 126, Iss , Pp 107637- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biodiversity hotspot Cherax quadricarinatus Environmental DNA Freshwater Invasive species qPCR Ecology QH540-549.5
spellingShingle	Biodiversity hotspot Cherax quadricarinatus Environmental DNA Freshwater Invasive species qPCR Ecology QH540-549.5 Thomas Baudry Quentin Mauvisseau Jean-Pierre Goût Alexandre Arqué Carine Delaunay Juliette Smith-Ravin Michael Sweet Frédéric Grandjean Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
description	The lesser Antilles archipelago in the Caribbean is known as a biodiversity hotspot, hosting many endemic species. However, recent introduction of a highly invasive species, the Australian redclaw crayfish (Cherax quadricarinatus), has led to significant threats to this fragile ecosystem. Here we developed, validated, and optimized a species-specific eDNA-based detection protocol targeting the 16S region of the mitochondrial gene of C. quadricarinatus. Our aim was to assess the crayfish distribution across Martinique Island. Our developed assay was species-specific and showed high sensitivity in laboratory, mesocosm and field conditions. A significant and positive correlation was found between species biomass, detection probability and efficiency through mesocosm experiments. Moreover, we found eDNA persisted up to 23 days in tropical freshwaters. We investigated a total of 83 locations, spread over 53 rivers and two closed water basins using our novel eDNA assay and traditional trapping, the latter, undertaken to confirm the reliability of the molecular-based detection method. Overall, we detected C. quadricarinatus at 47 locations using eDNA and 28 using traditional trapping, all positive trapping sites were positive for eDNA. We found that eDNA-based monitoring was less time-consuming and less influenced by the crayfishes often patchy distributions, proving a more reliable tool for future large-scale surveys. The clear threat and worrying distribution of this invasive species is particularly alarming as the archipelago belongs to one of the 25 identified biodiversity hotspots on Earth.
format	article
author	Thomas Baudry Quentin Mauvisseau Jean-Pierre Goût Alexandre Arqué Carine Delaunay Juliette Smith-Ravin Michael Sweet Frédéric Grandjean
author_facet	Thomas Baudry Quentin Mauvisseau Jean-Pierre Goût Alexandre Arqué Carine Delaunay Juliette Smith-Ravin Michael Sweet Frédéric Grandjean
author_sort	Thomas Baudry
title	Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
title_short	Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
title_full	Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
title_fullStr	Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
title_full_unstemmed	Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story
title_sort	mapping a super-invader in a biodiversity hotspot, an edna-based success story
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/19258642cb854b8b981b27e09daa242e
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Mapping a super-invader in a biodiversity hotspot, an eDNA-based success story

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