Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer

Here, we investigated the clinicopathological and prognostic potential of the long noncoding RNA Colon Cancer-Associated Transcript 2 (CCAT2) in human colorectal cancer (CRC). We used qPCR to quantify CCAT2 levels in 44 pairs of CRC tissues and adjacent nontumor and healthy colon mucosa tissues, and...

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Autores principales:	Dalu Wang, Zhilong Li, Hongzhuan Yin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	LncRNA CCAT2 colorectal cancer TAF15 RAB14 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/1928389286154308af95402fedb9656b
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spelling	oai:doaj.org-article:1928389286154308af95402fedb9656b2021-11-19T05:12:30ZLong Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer2234-943X10.3389/fonc.2021.751903https://doaj.org/article/1928389286154308af95402fedb9656b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.751903/fullhttps://doaj.org/toc/2234-943XHere, we investigated the clinicopathological and prognostic potential of the long noncoding RNA Colon Cancer-Associated Transcript 2 (CCAT2) in human colorectal cancer (CRC). We used qPCR to quantify CCAT2 levels in 44 pairs of CRC tissues and adjacent nontumor and healthy colon mucosa tissues, and in several CRC cell lines (SW620, SW480, HT-29, LOVO, HCT116 and DLD-1) and normal human colorectal epithelial cells (HFC). We assessed the effects of CCAT2 overexpression or knockdown on the proliferation, migration and invasion by SW620 and LOVO cells using CCK-8, transwell, and wound−healing assays, respectively. We also investigated the potential interaction between CCAT2 and TAF15 through RNA pull down and rescue experiments. Lastly, we evaluated the expression of the cell cycle progression markers and GSK3β signaling pathway proteins using Western blotting. Our results showed that CCAT2 was upregulated in CRC tissues and cell lines as com-pared to controls. Ectopic expression of CCAT2 promoted CRC cell proliferation, migration and invasion, likely through direct interaction with TAF15, transcriptional activation of RAB14, and activation of the AKT/GSK3β signaling pathway. In vivo, CCAT2 promoted CRC cell growth and metastasis in nude mice. Taken together, these results highlight the actions of CCAT2 as a CRC oncogene.Dalu WangZhilong LiHongzhuan YinFrontiers Media S.A.articleLncRNACCAT2colorectal cancerTAF15RAB14Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	LncRNA CCAT2 colorectal cancer TAF15 RAB14 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	LncRNA CCAT2 colorectal cancer TAF15 RAB14 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Dalu Wang Zhilong Li Hongzhuan Yin Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
description	Here, we investigated the clinicopathological and prognostic potential of the long noncoding RNA Colon Cancer-Associated Transcript 2 (CCAT2) in human colorectal cancer (CRC). We used qPCR to quantify CCAT2 levels in 44 pairs of CRC tissues and adjacent nontumor and healthy colon mucosa tissues, and in several CRC cell lines (SW620, SW480, HT-29, LOVO, HCT116 and DLD-1) and normal human colorectal epithelial cells (HFC). We assessed the effects of CCAT2 overexpression or knockdown on the proliferation, migration and invasion by SW620 and LOVO cells using CCK-8, transwell, and wound−healing assays, respectively. We also investigated the potential interaction between CCAT2 and TAF15 through RNA pull down and rescue experiments. Lastly, we evaluated the expression of the cell cycle progression markers and GSK3β signaling pathway proteins using Western blotting. Our results showed that CCAT2 was upregulated in CRC tissues and cell lines as com-pared to controls. Ectopic expression of CCAT2 promoted CRC cell proliferation, migration and invasion, likely through direct interaction with TAF15, transcriptional activation of RAB14, and activation of the AKT/GSK3β signaling pathway. In vivo, CCAT2 promoted CRC cell growth and metastasis in nude mice. Taken together, these results highlight the actions of CCAT2 as a CRC oncogene.
format	article
author	Dalu Wang Zhilong Li Hongzhuan Yin
author_facet	Dalu Wang Zhilong Li Hongzhuan Yin
author_sort	Dalu Wang
title	Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
title_short	Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
title_full	Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
title_fullStr	Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
title_full_unstemmed	Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer
title_sort	long non-coding rna ccat2 activates rab14 and acts as an oncogene in colorectal cancer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1928389286154308af95402fedb9656b
work_keys_str_mv	AT daluwang longnoncodingrnaccat2activatesrab14andactsasanoncogeneincolorectalcancer AT zhilongli longnoncodingrnaccat2activatesrab14andactsasanoncogeneincolorectalcancer AT hongzhuanyin longnoncodingrnaccat2activatesrab14andactsasanoncogeneincolorectalcancer
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Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer

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