Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents

Eli Fine1, Lijie Zhang1, Hicham Fenniri2, Thomas J Webster1 1Department of Engineering, Brown University, Providence, RI, USA; 2National Institute for Nanotechnology and Department of Chemistry, University of Alberta, Edmonton, AB, CanadaAbstract: One of the main problems with current vascular stent...

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Autores principales:	Eli Fine, Lijie Zhang, Hicham Fenniri, Thomas J Webster
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/192e55bbbc1a4ce0a1c88076a6b33dc6
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spelling	oai:doaj.org-article:192e55bbbc1a4ce0a1c88076a6b33dc62021-12-02T07:36:52ZEnhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents1176-91141178-2013https://doaj.org/article/192e55bbbc1a4ce0a1c88076a6b33dc62009-04-01T00:00:00Zhttp://www.dovepress.com/enhanced-endothelial-cell-functions-on-rosette-nanotube-coated-titaniu-a3055https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Eli Fine1, Lijie Zhang1, Hicham Fenniri2, Thomas J Webster1 1Department of Engineering, Brown University, Providence, RI, USA; 2National Institute for Nanotechnology and Department of Chemistry, University of Alberta, Edmonton, AB, CanadaAbstract: One of the main problems with current vascular stents is a lack of endothelial cell interactions, which if sufficient, would create a uniform healthy endothelium masking the underlying foreign metal from inflammatory cell interference. Moreover, if endothelial cells from the arterial wall do not adhere to the stent, the stent can become loose and dislodge. Therefore, the objective of this in vitro study was to design a novel biomimetic nanostructured coating (that does not contain drugs) on conventional vascular stent materials (specifically, titanium) for improving vascular stent applications. Rosette nanotubes (RNTs) are a new class of biomimetic nanotubes that self-assemble from DNA base analogs and have been shown in previous studies to sufficiently coat titanium and enhance osteoblast cell functions. RNTs have many desirable properties for use as vascular stent coatings including spontaneous self-assembly in body fluids, tailorable surface chemistry for specific implant applications, and nanoscale dimensions similar to those of the natural vascular extracellular matrix. Importantly, the results of this study provided the first evidence that RNTs functionalized with lysine (RNT&ndash;K), even at low concentrations, significantly increase endothelial cell density over uncoated titanium. Specifically, 0.01 mg/mL RNT&ndash;K coated titanium increased endothelial cell density by 37% and 52% compared to uncoated titanium after 4 h and three days, respectively. The excellent cytocompatibility properties of RNTs (as demonstrated here for the first time for endothelial cells) suggest the need for the further exploration of these novel nanostructured materials for vascular stent applications.Keywords: stents, nanotechnology, self-assembly, rosette nanotubes, endothelial cells, titanium Eli FineLijie ZhangHicham FenniriThomas J WebsterDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2009, Iss default, Pp 91-97 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Eli Fine Lijie Zhang Hicham Fenniri Thomas J Webster Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
description	Eli Fine1, Lijie Zhang1, Hicham Fenniri2, Thomas J Webster1 1Department of Engineering, Brown University, Providence, RI, USA; 2National Institute for Nanotechnology and Department of Chemistry, University of Alberta, Edmonton, AB, CanadaAbstract: One of the main problems with current vascular stents is a lack of endothelial cell interactions, which if sufficient, would create a uniform healthy endothelium masking the underlying foreign metal from inflammatory cell interference. Moreover, if endothelial cells from the arterial wall do not adhere to the stent, the stent can become loose and dislodge. Therefore, the objective of this in vitro study was to design a novel biomimetic nanostructured coating (that does not contain drugs) on conventional vascular stent materials (specifically, titanium) for improving vascular stent applications. Rosette nanotubes (RNTs) are a new class of biomimetic nanotubes that self-assemble from DNA base analogs and have been shown in previous studies to sufficiently coat titanium and enhance osteoblast cell functions. RNTs have many desirable properties for use as vascular stent coatings including spontaneous self-assembly in body fluids, tailorable surface chemistry for specific implant applications, and nanoscale dimensions similar to those of the natural vascular extracellular matrix. Importantly, the results of this study provided the first evidence that RNTs functionalized with lysine (RNT&ndash;K), even at low concentrations, significantly increase endothelial cell density over uncoated titanium. Specifically, 0.01 mg/mL RNT&ndash;K coated titanium increased endothelial cell density by 37% and 52% compared to uncoated titanium after 4 h and three days, respectively. The excellent cytocompatibility properties of RNTs (as demonstrated here for the first time for endothelial cells) suggest the need for the further exploration of these novel nanostructured materials for vascular stent applications.Keywords: stents, nanotechnology, self-assembly, rosette nanotubes, endothelial cells, titanium
format	article
author	Eli Fine Lijie Zhang Hicham Fenniri Thomas J Webster
author_facet	Eli Fine Lijie Zhang Hicham Fenniri Thomas J Webster
author_sort	Eli Fine
title	Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
title_short	Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
title_full	Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
title_fullStr	Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
title_full_unstemmed	Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
title_sort	enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/192e55bbbc1a4ce0a1c88076a6b33dc6
work_keys_str_mv	AT elifine enhancedendothelialcellfunctionsonrosettenanotubecoatedtitaniumvascularstents AT lijiezhang enhancedendothelialcellfunctionsonrosettenanotubecoatedtitaniumvascularstents AT hichamfenniri enhancedendothelialcellfunctionsonrosettenanotubecoatedtitaniumvascularstents AT thomasjwebster enhancedendothelialcellfunctionsonrosettenanotubecoatedtitaniumvascularstents
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Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents

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