Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect

Hypertension is one of the important causes of cardiovascular diseases, and the imbalance of vascular homeostasis caused by oxidative stress and endothelial inflammation occurs throughout hypertension pathogenesis. Therefore, inhibiting oxidative stress and endothelial inflammation is important for...

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Autores principales:	Linhua Deng, Wei Liu, Qiang Xu, Rui Guo, Dapeng Zhang, Jingyu Ni, Lan Li, Xiaoyue Cai, Guanwei Fan, Yingqiang Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Tianma Gouteng Decoction Chinese medicine Hypertension TFEB Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/19364e39ea704e5e9865e162ae2ea52b
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spelling	oai:doaj.org-article:19364e39ea704e5e9865e162ae2ea52b2021-11-04T04:26:20ZTianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect0753-332210.1016/j.biopha.2021.112383https://doaj.org/article/19364e39ea704e5e9865e162ae2ea52b2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011677https://doaj.org/toc/0753-3322Hypertension is one of the important causes of cardiovascular diseases, and the imbalance of vascular homeostasis caused by oxidative stress and endothelial inflammation occurs throughout hypertension pathogenesis. Therefore, inhibiting oxidative stress and endothelial inflammation is important for treating hypertension. Tianma Gouteng Decoction (TGD) is a Chinese herbal medicine that is commonly used to treat hypertension in China, and demonstrates clinically effective antihypertensive effects. However, its blood pressure reduction mechanism remains unclear. In this study, we further determined the antihypertensive effects of TGD and revealed its underlying mechanism. We established an AngII-induced hypertension mice model, which was treated with TGD for six weeks. We monitored blood pressure, heart rate, and body weight every week. After six weeks, we detected changes in the structure and function of the heart, the structure of blood vessels, and vasomotor factors. We also detected the expression of oxidative stress and inflammation-related genes. We found that TGD can significantly reduce blood pressure, improve cardiac structure and function, and reverse vascular remodeling, which could be due to the inhibition of oxidative stress and inflammation. We also found that the effect of inhibiting oxidative stress and inflammation could be related to the up-regulation of transcription factor EB (TFEB) expression by TGD. Therefore, we used AAV9 to knock down TFEB and observe the role of TFEB in TGD's antihypertensive and cardiovascular protection properties. We found that after TFEB knockdown, the protective effect of TGD on blood pressure and cardiovascular remodeling in AngII-induced hypertensive mice was inhibited, and that it was unable to inhibit oxidative stress and inflammation. Therefore, our study demonstrated for the first time that TGD could exert anti-oxidative stress and anti-inflammatory effects through TFEB and reverse the cardiovascular remodeling caused by hypertension.Linhua DengWei LiuQiang XuRui GuoDapeng ZhangJingyu NiLan LiXiaoyue CaiGuanwei FanYingqiang ZhaoElsevierarticleTianma Gouteng DecoctionChinese medicineHypertensionTFEBTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112383- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tianma Gouteng Decoction Chinese medicine Hypertension TFEB Therapeutics. Pharmacology RM1-950
spellingShingle	Tianma Gouteng Decoction Chinese medicine Hypertension TFEB Therapeutics. Pharmacology RM1-950 Linhua Deng Wei Liu Qiang Xu Rui Guo Dapeng Zhang Jingyu Ni Lan Li Xiaoyue Cai Guanwei Fan Yingqiang Zhao Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
description	Hypertension is one of the important causes of cardiovascular diseases, and the imbalance of vascular homeostasis caused by oxidative stress and endothelial inflammation occurs throughout hypertension pathogenesis. Therefore, inhibiting oxidative stress and endothelial inflammation is important for treating hypertension. Tianma Gouteng Decoction (TGD) is a Chinese herbal medicine that is commonly used to treat hypertension in China, and demonstrates clinically effective antihypertensive effects. However, its blood pressure reduction mechanism remains unclear. In this study, we further determined the antihypertensive effects of TGD and revealed its underlying mechanism. We established an AngII-induced hypertension mice model, which was treated with TGD for six weeks. We monitored blood pressure, heart rate, and body weight every week. After six weeks, we detected changes in the structure and function of the heart, the structure of blood vessels, and vasomotor factors. We also detected the expression of oxidative stress and inflammation-related genes. We found that TGD can significantly reduce blood pressure, improve cardiac structure and function, and reverse vascular remodeling, which could be due to the inhibition of oxidative stress and inflammation. We also found that the effect of inhibiting oxidative stress and inflammation could be related to the up-regulation of transcription factor EB (TFEB) expression by TGD. Therefore, we used AAV9 to knock down TFEB and observe the role of TFEB in TGD's antihypertensive and cardiovascular protection properties. We found that after TFEB knockdown, the protective effect of TGD on blood pressure and cardiovascular remodeling in AngII-induced hypertensive mice was inhibited, and that it was unable to inhibit oxidative stress and inflammation. Therefore, our study demonstrated for the first time that TGD could exert anti-oxidative stress and anti-inflammatory effects through TFEB and reverse the cardiovascular remodeling caused by hypertension.
format	article
author	Linhua Deng Wei Liu Qiang Xu Rui Guo Dapeng Zhang Jingyu Ni Lan Li Xiaoyue Cai Guanwei Fan Yingqiang Zhao
author_facet	Linhua Deng Wei Liu Qiang Xu Rui Guo Dapeng Zhang Jingyu Ni Lan Li Xiaoyue Cai Guanwei Fan Yingqiang Zhao
author_sort	Linhua Deng
title	Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
title_short	Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
title_full	Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
title_fullStr	Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
title_full_unstemmed	Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect
title_sort	tianma gouteng decoction regulates oxidative stress and inflammation in angii-induced hypertensive mice via transcription factor eb to exert anti-hypertension effect
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/19364e39ea704e5e9865e162ae2ea52b
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Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti-hypertension effect

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