Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to...

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Autores principales:	Pôrto Regiani Nascimento Gagno, Junqueira-Kipnis Ana Paula, de Oliveira Viu Marco Antonio, Teixeira Rafaela Cavalcanti, Gambarini Maria Lucia
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	cow genital micoplasmosis innate immunity infection Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/1941d4f4eff94067af9a885cd8f7d688
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spelling	oai:doaj.org-article:1941d4f4eff94067af9a885cd8f7d6882021-11-17T21:27:54ZEvaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum1820-744810.2478/acve-2021-0012https://doaj.org/article/1941d4f4eff94067af9a885cd8f7d6882021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0012https://doaj.org/toc/1820-7448Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confirmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.Pôrto Regiani Nascimento GagnoJunqueira-Kipnis Ana Paulade Oliveira Viu Marco AntonioTeixeira Rafaela CavalcantiGambarini Maria LuciaSciendoarticlecowgenital micoplasmosisinnate immunityinfectionVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 137-146 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cow genital micoplasmosis innate immunity infection Veterinary medicine SF600-1100
spellingShingle	cow genital micoplasmosis innate immunity infection Veterinary medicine SF600-1100 Pôrto Regiani Nascimento Gagno Junqueira-Kipnis Ana Paula de Oliveira Viu Marco Antonio Teixeira Rafaela Cavalcanti Gambarini Maria Lucia Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
description	Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confirmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.
format	article
author	Pôrto Regiani Nascimento Gagno Junqueira-Kipnis Ana Paula de Oliveira Viu Marco Antonio Teixeira Rafaela Cavalcanti Gambarini Maria Lucia
author_facet	Pôrto Regiani Nascimento Gagno Junqueira-Kipnis Ana Paula de Oliveira Viu Marco Antonio Teixeira Rafaela Cavalcanti Gambarini Maria Lucia
author_sort	Pôrto Regiani Nascimento Gagno
title	Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
title_short	Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
title_full	Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
title_fullStr	Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
title_full_unstemmed	Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum
title_sort	evaluation of in vitro activation of bovine endometrial and vaginal epithelial and blood mononuclear cells to produce nitric oxide in response to mycoplasma bovis, mycoplasma bovigenitalium and ureaplasma diversum
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/1941d4f4eff94067af9a885cd8f7d688
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Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

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