Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats

Objective. Trans-chalcone is a chalcone with hepatoprotective and anti-inflammatory effects. However, the mechanism of these positive effects, especially on miR-451 as an inflammatory regulator, is poorly understood. In this regard, this microRNA (miRNA) acts by inhibition of hepatic interleukin-8 (...

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Autores principales:	Karimi-Sales Elham, Jeddi Sajad, Ghaffari-Nasab Arshad, Salimi Mina, Alipour Mohammad Reza
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	trans-chalcone mir-451 interleukin-8 liver Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/1943fcfe4efe4942aa5736934cb05b45
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spelling	oai:doaj.org-article:1943fcfe4efe4942aa5736934cb05b452021-12-02T16:41:13ZEffect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats1336-032910.2478/enr-2018-0001https://doaj.org/article/1943fcfe4efe4942aa5736934cb05b452018-01-01T00:00:00Zhttps://doi.org/10.2478/enr-2018-0001https://doaj.org/toc/1336-0329Objective. Trans-chalcone is a chalcone with hepatoprotective and anti-inflammatory effects. However, the mechanism of these positive effects, especially on miR-451 as an inflammatory regulator, is poorly understood. In this regard, this microRNA (miRNA) acts by inhibition of hepatic interleukin-8 (IL-8) production in the liver which is one of the main proinflammatory cytokines. Th is study for the first time examined the effect of trans-chalcone on miR-451/IL-8 pathway. Methods. In present study, 21 male rats were randomly divided into 3 groups (n=7 per each group): control which received solvent (NS), groups 2 (N2T) and 3 (N6T), which received transchalcone for 2 and 6 weeks, respectively. Hepatic level of miR-451 was measured by qRT-PCR. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as hepatic level of IL-8 protein were measured. Results. Trans-chalcone decreased hepatic level of IL-8 protein and serum level of ALT aft er 2 weeks of treatment without significant change in hepatic miR-451. Moreover, it increased hepatic level of miR-451 and reduced hepatic IL-8 as well as AST and ALT aft er 6 weeks. Conclusion. Based on the results of present study, miR-451/IL-8 pathway is a possible mechanism for hepatoprotective action of trans-chalcone in long-term.Karimi-Sales ElhamJeddi SajadGhaffari-Nasab ArshadSalimi MinaAlipour Mohammad RezaSciendoarticletrans-chalconemir-451interleukin-8liverDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENEndocrine Regulations, Vol 52, Iss 1, Pp 1-5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	trans-chalcone mir-451 interleukin-8 liver Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	trans-chalcone mir-451 interleukin-8 liver Diseases of the endocrine glands. Clinical endocrinology RC648-665 Karimi-Sales Elham Jeddi Sajad Ghaffari-Nasab Arshad Salimi Mina Alipour Mohammad Reza Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
description	Objective. Trans-chalcone is a chalcone with hepatoprotective and anti-inflammatory effects. However, the mechanism of these positive effects, especially on miR-451 as an inflammatory regulator, is poorly understood. In this regard, this microRNA (miRNA) acts by inhibition of hepatic interleukin-8 (IL-8) production in the liver which is one of the main proinflammatory cytokines. Th is study for the first time examined the effect of trans-chalcone on miR-451/IL-8 pathway. Methods. In present study, 21 male rats were randomly divided into 3 groups (n=7 per each group): control which received solvent (NS), groups 2 (N2T) and 3 (N6T), which received transchalcone for 2 and 6 weeks, respectively. Hepatic level of miR-451 was measured by qRT-PCR. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as hepatic level of IL-8 protein were measured. Results. Trans-chalcone decreased hepatic level of IL-8 protein and serum level of ALT aft er 2 weeks of treatment without significant change in hepatic miR-451. Moreover, it increased hepatic level of miR-451 and reduced hepatic IL-8 as well as AST and ALT aft er 6 weeks. Conclusion. Based on the results of present study, miR-451/IL-8 pathway is a possible mechanism for hepatoprotective action of trans-chalcone in long-term.
format	article
author	Karimi-Sales Elham Jeddi Sajad Ghaffari-Nasab Arshad Salimi Mina Alipour Mohammad Reza
author_facet	Karimi-Sales Elham Jeddi Sajad Ghaffari-Nasab Arshad Salimi Mina Alipour Mohammad Reza
author_sort	Karimi-Sales Elham
title	Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
title_short	Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
title_full	Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
title_fullStr	Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
title_full_unstemmed	Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats
title_sort	effect of trans-chalcone on hepatic il-8 through the regulation of mir-451 in male rats
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/1943fcfe4efe4942aa5736934cb05b45
work_keys_str_mv	AT karimisaleselham effectoftranschalconeonhepaticil8throughtheregulationofmir451inmalerats AT jeddisajad effectoftranschalconeonhepaticil8throughtheregulationofmir451inmalerats AT ghaffarinasabarshad effectoftranschalconeonhepaticil8throughtheregulationofmir451inmalerats AT salimimina effectoftranschalconeonhepaticil8throughtheregulationofmir451inmalerats AT alipourmohammadreza effectoftranschalconeonhepaticil8throughtheregulationofmir451inmalerats
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Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats

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