Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients

Factor V Leiden G1691A (FVL) and Factor II prothrombin G20210A (PGM) mutations are the leading causes of thrombophilia. In this study, we have investigated the prevalence of the FVL G1691A and PGM G20210A single nucleotide polymorphisms (SNPs) among Libyan deep vein thrombosis (DVT) and myocardial i...

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Autores principales:	Abdulghani Msalati, Abdulla Bashein, Murad Ghrew, Ibtesam Khalil, Khaled Sedaa, Abushawashi Ali, Ahmed Zaid
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	gene polymorphisms thrombophilia factor v leiden prothrombin g20210a Medicine R
Acceso en línea:	https://doaj.org/article/1947beac30ef4516b36a5b781d79f185
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spelling	oai:doaj.org-article:1947beac30ef4516b36a5b781d79f1852021-11-26T11:19:48ZAssociation of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients1993-28201819-635710.1080/19932820.2020.1857525https://doaj.org/article/1947beac30ef4516b36a5b781d79f1852021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19932820.2020.1857525https://doaj.org/toc/1993-2820https://doaj.org/toc/1819-6357Factor V Leiden G1691A (FVL) and Factor II prothrombin G20210A (PGM) mutations are the leading causes of thrombophilia. In this study, we have investigated the prevalence of the FVL G1691A and PGM G20210A single nucleotide polymorphisms (SNPs) among Libyan deep vein thrombosis (DVT) and myocardial infarction (MI) patients. SNP genotyping was performed using high-resolution melt analysis (HRM) and DNA sequencing. Biochemical parameters conducted on 112 males and 93 females showed no significant difference in means between the control group and the deep vein thrombosis and myocardial infarction groups. For Factor V Leiden, 40 samples were genotyped. Of the 40 samples, 6 (15.0%) of them were heterozygous and no one was homozygous. As for Factor II SNP, 59 samples were genotyped and only 2 (3.3%) were heterozygous. All the heterozygous samples showed 100% concordance between the HRM-PCR and DNA sequence analysis. Our study showed, for the first time, that both the FVL and PGM mutations are present among Libyan DVT and MI patients and that the FVL mutation is significantly associated with DVT but not with MI. However, our results do not support the association of PGM G20210A mutation with DVT or MI.Abdulghani MsalatiAbdulla BasheinMurad GhrewIbtesam KhalilKhaled SedaaAbushawashi AliAhmed ZaidTaylor & Francis Grouparticlegene polymorphismsthrombophiliafactor v leidenprothrombin g20210aMedicineRENLibyan Journal of Medicine, Vol 16, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene polymorphisms thrombophilia factor v leiden prothrombin g20210a Medicine R
spellingShingle	gene polymorphisms thrombophilia factor v leiden prothrombin g20210a Medicine R Abdulghani Msalati Abdulla Bashein Murad Ghrew Ibtesam Khalil Khaled Sedaa Abushawashi Ali Ahmed Zaid Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
description	Factor V Leiden G1691A (FVL) and Factor II prothrombin G20210A (PGM) mutations are the leading causes of thrombophilia. In this study, we have investigated the prevalence of the FVL G1691A and PGM G20210A single nucleotide polymorphisms (SNPs) among Libyan deep vein thrombosis (DVT) and myocardial infarction (MI) patients. SNP genotyping was performed using high-resolution melt analysis (HRM) and DNA sequencing. Biochemical parameters conducted on 112 males and 93 females showed no significant difference in means between the control group and the deep vein thrombosis and myocardial infarction groups. For Factor V Leiden, 40 samples were genotyped. Of the 40 samples, 6 (15.0%) of them were heterozygous and no one was homozygous. As for Factor II SNP, 59 samples were genotyped and only 2 (3.3%) were heterozygous. All the heterozygous samples showed 100% concordance between the HRM-PCR and DNA sequence analysis. Our study showed, for the first time, that both the FVL and PGM mutations are present among Libyan DVT and MI patients and that the FVL mutation is significantly associated with DVT but not with MI. However, our results do not support the association of PGM G20210A mutation with DVT or MI.
format	article
author	Abdulghani Msalati Abdulla Bashein Murad Ghrew Ibtesam Khalil Khaled Sedaa Abushawashi Ali Ahmed Zaid
author_facet	Abdulghani Msalati Abdulla Bashein Murad Ghrew Ibtesam Khalil Khaled Sedaa Abushawashi Ali Ahmed Zaid
author_sort	Abdulghani Msalati
title	Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
title_short	Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
title_full	Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
title_fullStr	Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
title_full_unstemmed	Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
title_sort	association of venous thromboembolism and myocardial infarction with factor v leiden and factor ii gene mutations among libyan patients
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1947beac30ef4516b36a5b781d79f185
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Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients

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