Molecular Survey of Babesia spp. and Anaplasma phagocytophilum in Roe Deer from a Wildlife Rescue Center in Italy

Babesia ssp. and Anaplasma spp. are tick-borne microorganisms representing a possible health risk for domestic and wild animals, as well as humans. Roe deer serve as a suitable reservoir host for some species ascribed to Babesia spp. and <i...

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Autores principales:	Alessandra Cafiso, Chiara Bazzocchi, Martina Cavagna, Elena Di Lorenzo, Valentina Serra, Riccardo Rossi, Stefano Comazzi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Babesia</i> <i>Anaplasma phagocytophilum</i> rescued animal roe deer zoonosis tick-borne pathogen Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/194f8414d6b64c5d9f7cb99dbb395289
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spelling	oai:doaj.org-article:194f8414d6b64c5d9f7cb99dbb3952892021-11-25T16:21:45ZMolecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy10.3390/ani111133352076-2615https://doaj.org/article/194f8414d6b64c5d9f7cb99dbb3952892021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3335https://doaj.org/toc/2076-2615<i>Babesia</i> ssp. and <i>Anaplasma</i> spp. are tick-borne microorganisms representing a possible health risk for domestic and wild animals, as well as humans. Roe deer serve as a suitable reservoir host for some species ascribed to <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> taxa, also due to its important role in the maintenance of large populations of <i>Ixodes ricinus</i>, the main tick vector of these pathogens in Europe. Roe deer populations have been recently expanding throughout Europe, namely in Italy. However, the collection of samples from free-ranging wild animals for diagnostic investigations often includes several practical issues. This problem can be overcome using samples provided by wildlife rescue centers making them available for investigations following routine analyses. The presence of <i>Babesia</i> spp. and <i>Anaplasma</i> spp. in blood samples of 43 roe deer rescued by a wildlife rescue center in Emilia-Romagna region (Italy) was molecularly investigated. PCR screening revealed the presence of at least one pathogen in 86.05% of the animals, while co-infection occurred in 18.92% of the tested individuals. Zoonotic <i>Babesia venatorum</i> was found in 6.98% of the samples, while <i>Babesia capreoli</i> and <i>Anaplasma phagocytophilum</i> were detected in 74.42% and in 20.93%, respectively. No hematological signs compatible with clinical anaplasmosis or piroplasmosis, as well as absence of intracellular circulating microorganisms in blood smears, were observed, suggesting asymptomatic infection in the tested animals. These results confirm the usefulness of wild rescued animals as convenient source of biological samples for tick-borne pathogens investigation and the role of roe deer as a key factor in the endemic cycle of <i>Babesia</i> species and <i>A. phagocytophilum</i>.Alessandra CafisoChiara BazzocchiMartina CavagnaElena Di LorenzoValentina SerraRiccardo RossiStefano ComazziMDPI AGarticle<i>Babesia</i><i>Anaplasma phagocytophilum</i>rescued animalroe deerzoonosistick-borne pathogenVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3335, p 3335 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Babesia</i> <i>Anaplasma phagocytophilum</i> rescued animal roe deer zoonosis tick-borne pathogen Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	<i>Babesia</i> <i>Anaplasma phagocytophilum</i> rescued animal roe deer zoonosis tick-borne pathogen Veterinary medicine SF600-1100 Zoology QL1-991 Alessandra Cafiso Chiara Bazzocchi Martina Cavagna Elena Di Lorenzo Valentina Serra Riccardo Rossi Stefano Comazzi Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
description	<i>Babesia</i> ssp. and <i>Anaplasma</i> spp. are tick-borne microorganisms representing a possible health risk for domestic and wild animals, as well as humans. Roe deer serve as a suitable reservoir host for some species ascribed to <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> taxa, also due to its important role in the maintenance of large populations of <i>Ixodes ricinus</i>, the main tick vector of these pathogens in Europe. Roe deer populations have been recently expanding throughout Europe, namely in Italy. However, the collection of samples from free-ranging wild animals for diagnostic investigations often includes several practical issues. This problem can be overcome using samples provided by wildlife rescue centers making them available for investigations following routine analyses. The presence of <i>Babesia</i> spp. and <i>Anaplasma</i> spp. in blood samples of 43 roe deer rescued by a wildlife rescue center in Emilia-Romagna region (Italy) was molecularly investigated. PCR screening revealed the presence of at least one pathogen in 86.05% of the animals, while co-infection occurred in 18.92% of the tested individuals. Zoonotic <i>Babesia venatorum</i> was found in 6.98% of the samples, while <i>Babesia capreoli</i> and <i>Anaplasma phagocytophilum</i> were detected in 74.42% and in 20.93%, respectively. No hematological signs compatible with clinical anaplasmosis or piroplasmosis, as well as absence of intracellular circulating microorganisms in blood smears, were observed, suggesting asymptomatic infection in the tested animals. These results confirm the usefulness of wild rescued animals as convenient source of biological samples for tick-borne pathogens investigation and the role of roe deer as a key factor in the endemic cycle of <i>Babesia</i> species and <i>A. phagocytophilum</i>.
format	article
author	Alessandra Cafiso Chiara Bazzocchi Martina Cavagna Elena Di Lorenzo Valentina Serra Riccardo Rossi Stefano Comazzi
author_facet	Alessandra Cafiso Chiara Bazzocchi Martina Cavagna Elena Di Lorenzo Valentina Serra Riccardo Rossi Stefano Comazzi
author_sort	Alessandra Cafiso
title	Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
title_short	Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
title_full	Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
title_fullStr	Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
title_full_unstemmed	Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy
title_sort	molecular survey of <i>babesia</i> spp. and <i>anaplasma phagocytophilum</i> in roe deer from a wildlife rescue center in italy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/194f8414d6b64c5d9f7cb99dbb395289
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Molecular Survey of <i>Babesia</i> spp. and <i>Anaplasma phagocytophilum</i> in Roe Deer from a Wildlife Rescue Center in Italy

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