Tissue and serum microRNA profile of oral squamous cell carcinoma patients

Abstract Head and neck cancer is characterized by malignant tumors arising from the epithelium covering the upper aerodigestive tract, and the majority of these epithelial malignancies are squamous cell carcinomas (SCCs) of the oral cavity (OSCCs). The aim of the current work was to identify miRNAs...

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Autores principales:	Augusto Schneider, Berta Victoria, Yury Nunez Lopez, Wiktoria Suchorska, Wojciech Barczak, Agnieszka Sobecka, Wojciech Golusinski, Michal M. Masternak, Pawel Golusinski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19554e1e0273454f8a58dfad9ec62d87
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spelling	oai:doaj.org-article:19554e1e0273454f8a58dfad9ec62d872021-12-02T15:08:55ZTissue and serum microRNA profile of oral squamous cell carcinoma patients10.1038/s41598-017-18945-z2045-2322https://doaj.org/article/19554e1e0273454f8a58dfad9ec62d872018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18945-zhttps://doaj.org/toc/2045-2322Abstract Head and neck cancer is characterized by malignant tumors arising from the epithelium covering the upper aerodigestive tract, and the majority of these epithelial malignancies are squamous cell carcinomas (SCCs) of the oral cavity (OSCCs). The aim of the current work was to identify miRNAs regulated in OSCC cancerous tissue when compared to a healthy adjacent tissue and to verify the presence of the same miRNAs in the circulation of these patients. For that serum samples and biopsies of healthy and tumor tissues were collected from five patients diagnosed with OSCC of the oral cavity, RNA was extracted from these samples and microRNAs libraries were prepared and sequenced. A total 255 miRNAs were identified in tissue and 381 different miRNAs were identified in serum samples. When comparing the miRNA expression between tumor and healthy tissue we identified 48 miRNAs (25 down- and 23 up-regulated) that were differentially expressed (FDR < 0.05). From these 48 differentially expressed miRNAs in tissue, 30 miRNAs were also found in the serum of the same patients. hsa-miR-32-5p was up-regulated in tumor compared to healthy tissue in our study, and was previously shown to be up-regulated in the serum of OSCC patients. Therefore, this suggests that miRNAs can be used as potential non-invasive biomarkers of OSCC.Augusto SchneiderBerta VictoriaYury Nunez LopezWiktoria SuchorskaWojciech BarczakAgnieszka SobeckaWojciech GolusinskiMichal M. MasternakPawel GolusinskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Augusto Schneider Berta Victoria Yury Nunez Lopez Wiktoria Suchorska Wojciech Barczak Agnieszka Sobecka Wojciech Golusinski Michal M. Masternak Pawel Golusinski Tissue and serum microRNA profile of oral squamous cell carcinoma patients
description	Abstract Head and neck cancer is characterized by malignant tumors arising from the epithelium covering the upper aerodigestive tract, and the majority of these epithelial malignancies are squamous cell carcinomas (SCCs) of the oral cavity (OSCCs). The aim of the current work was to identify miRNAs regulated in OSCC cancerous tissue when compared to a healthy adjacent tissue and to verify the presence of the same miRNAs in the circulation of these patients. For that serum samples and biopsies of healthy and tumor tissues were collected from five patients diagnosed with OSCC of the oral cavity, RNA was extracted from these samples and microRNAs libraries were prepared and sequenced. A total 255 miRNAs were identified in tissue and 381 different miRNAs were identified in serum samples. When comparing the miRNA expression between tumor and healthy tissue we identified 48 miRNAs (25 down- and 23 up-regulated) that were differentially expressed (FDR < 0.05). From these 48 differentially expressed miRNAs in tissue, 30 miRNAs were also found in the serum of the same patients. hsa-miR-32-5p was up-regulated in tumor compared to healthy tissue in our study, and was previously shown to be up-regulated in the serum of OSCC patients. Therefore, this suggests that miRNAs can be used as potential non-invasive biomarkers of OSCC.
format	article
author	Augusto Schneider Berta Victoria Yury Nunez Lopez Wiktoria Suchorska Wojciech Barczak Agnieszka Sobecka Wojciech Golusinski Michal M. Masternak Pawel Golusinski
author_facet	Augusto Schneider Berta Victoria Yury Nunez Lopez Wiktoria Suchorska Wojciech Barczak Agnieszka Sobecka Wojciech Golusinski Michal M. Masternak Pawel Golusinski
author_sort	Augusto Schneider
title	Tissue and serum microRNA profile of oral squamous cell carcinoma patients
title_short	Tissue and serum microRNA profile of oral squamous cell carcinoma patients
title_full	Tissue and serum microRNA profile of oral squamous cell carcinoma patients
title_fullStr	Tissue and serum microRNA profile of oral squamous cell carcinoma patients
title_full_unstemmed	Tissue and serum microRNA profile of oral squamous cell carcinoma patients
title_sort	tissue and serum microrna profile of oral squamous cell carcinoma patients
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/19554e1e0273454f8a58dfad9ec62d87
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Tissue and serum microRNA profile of oral squamous cell carcinoma patients

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