Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236

Abstract Chloroplasts are divided into six subcompartments: the outer membrane, intermembrane space, and inner membrane of the envelope, the stroma, the thylakoid membrane, and the thylakoid lumen. Compared with our knowledge of protein import into other subcompartments, extremely little is known ab...

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Autores principales:	Meng‐Rong Chuang, Lih‐Jen Chen, Hsou‐min Li
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	chloroplast envelope intermembrane space mar1 protein transport Tic22 Tic236 Botany QK1-989
Acceso en línea:	https://doaj.org/article/19555641e8b94d5da16b683e3240d26e
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spelling	oai:doaj.org-article:19555641e8b94d5da16b683e3240d26e2021-11-29T07:25:55ZChloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic2362475-445510.1002/pld3.356https://doaj.org/article/19555641e8b94d5da16b683e3240d26e2021-11-01T00:00:00Zhttps://doi.org/10.1002/pld3.356https://doaj.org/toc/2475-4455Abstract Chloroplasts are divided into six subcompartments: the outer membrane, intermembrane space, and inner membrane of the envelope, the stroma, the thylakoid membrane, and the thylakoid lumen. Compared with our knowledge of protein import into other subcompartments, extremely little is known about how proteins are imported into the intermembrane space of the envelope. Tic22 was one of the first proteins identified as localizing to the intermembrane space and the only one for which import has been analyzed in some detail. However, conflicting results have been obtained concerning whether the general translocon is used to import Tic22 into the intermembrane space. Taking advantage of available translocon component mutants, we reanalyzed import of Tic22. We reveal reduced in vitro import of Tic22 preprotein (prTic22) into chloroplasts isolated from the Arabidopsis mar1 and tic236 mutants, which are functional knockdown mutants of the outer‐membrane channel Toc75 and the intermembrane space linker Tic236, respectively. Import competition experiments also showed that prTic22 import was reduced by excess amounts of a stroma‐targeted preprotein. Our results indicate that prTic22 uses at least part of the general translocon for import into the intermembrane space.Meng‐Rong ChuangLih‐Jen ChenHsou‐min LiWileyarticlechloroplast envelopeintermembrane spacemar1protein transportTic22Tic236BotanyQK1-989ENPlant Direct, Vol 5, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chloroplast envelope intermembrane space mar1 protein transport Tic22 Tic236 Botany QK1-989
spellingShingle	chloroplast envelope intermembrane space mar1 protein transport Tic22 Tic236 Botany QK1-989 Meng‐Rong Chuang Lih‐Jen Chen Hsou‐min Li Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
description	Abstract Chloroplasts are divided into six subcompartments: the outer membrane, intermembrane space, and inner membrane of the envelope, the stroma, the thylakoid membrane, and the thylakoid lumen. Compared with our knowledge of protein import into other subcompartments, extremely little is known about how proteins are imported into the intermembrane space of the envelope. Tic22 was one of the first proteins identified as localizing to the intermembrane space and the only one for which import has been analyzed in some detail. However, conflicting results have been obtained concerning whether the general translocon is used to import Tic22 into the intermembrane space. Taking advantage of available translocon component mutants, we reanalyzed import of Tic22. We reveal reduced in vitro import of Tic22 preprotein (prTic22) into chloroplasts isolated from the Arabidopsis mar1 and tic236 mutants, which are functional knockdown mutants of the outer‐membrane channel Toc75 and the intermembrane space linker Tic236, respectively. Import competition experiments also showed that prTic22 import was reduced by excess amounts of a stroma‐targeted preprotein. Our results indicate that prTic22 uses at least part of the general translocon for import into the intermembrane space.
format	article
author	Meng‐Rong Chuang Lih‐Jen Chen Hsou‐min Li
author_facet	Meng‐Rong Chuang Lih‐Jen Chen Hsou‐min Li
author_sort	Meng‐Rong Chuang
title	Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
title_short	Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
title_full	Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
title_fullStr	Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
title_full_unstemmed	Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236
title_sort	chloroplast import of an intermembrane space protein is facilitated by translocon components toc75 and tic236
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/19555641e8b94d5da16b683e3240d26e
work_keys_str_mv	AT mengrongchuang chloroplastimportofanintermembranespaceproteinisfacilitatedbytransloconcomponentstoc75andtic236 AT lihjenchen chloroplastimportofanintermembranespaceproteinisfacilitatedbytransloconcomponentstoc75andtic236 AT hsouminli chloroplastimportofanintermembranespaceproteinisfacilitatedbytransloconcomponentstoc75andtic236
_version_	1718407510214311936

Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236

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