Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile

Abstract The tumoral origin and extensive passaging of HeLa cells, a most commonly used cervical epithelial cell line, raise concerns on their suitability to study the cell responses to infection. The present study was designed to isolate primary epithelial cells from human ectocervix explants and c...

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Autores principales:	Chongfa Tang, Chang Liu, Benoit Maffei, Béatrice Niragire, Henri Cohen, Aminata Kane, Anne-Claire Donnadieu, Yael Levy-Zauberman, Thomas Vernay, Juliette Hugueny, Etienne Vincens, Christine Louis-Sylvestre, Agathe Subtil, Yongzheng Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/195d3f7cbfb64e4db7b039c292bd24fa
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spelling	oai:doaj.org-article:195d3f7cbfb64e4db7b039c292bd24fa2021-12-02T13:15:51ZPrimary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile10.1038/s41598-021-85123-72045-2322https://doaj.org/article/195d3f7cbfb64e4db7b039c292bd24fa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85123-7https://doaj.org/toc/2045-2322Abstract The tumoral origin and extensive passaging of HeLa cells, a most commonly used cervical epithelial cell line, raise concerns on their suitability to study the cell responses to infection. The present study was designed to isolate primary epithelial cells from human ectocervix explants and characterize their susceptibility to C. trachomatis infection. We achieved a high purity of isolation, assessed by the expression of E-cadherin and cytokeratin 14. The infectious progeny in these primary epithelial cells was lower than in HeLa cells. We showed that the difference in culture medium, and the addition of serum in HeLa cultures, accounted for a large part of these differences. However, all things considered the primary ectocervical epithelial cells remained less permissive than HeLa cells to C. trachomatis serovar L2 or D development. Finally, the basal level of transcription of genes coding for pro-inflammatory cytokines was globally higher in primary epithelial cells than in HeLa cells. Transcription of several pro-inflammatory genes was further induced by infection with C. trachomatis serovar L2 or serovar D. In conclusion, primary epithelial cells have a strong capacity to mount an inflammatory response to Chlamydia infection. Our simplified purification protocol from human explants should facilitate future studies to understand the contribution of this response to limiting the spread of the pathogen to the upper female genital tract.Chongfa TangChang LiuBenoit MaffeiBéatrice NiragireHenri CohenAminata KaneAnne-Claire DonnadieuYael Levy-ZaubermanThomas VernayJuliette HuguenyEtienne VincensChristine Louis-SylvestreAgathe SubtilYongzheng WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chongfa Tang Chang Liu Benoit Maffei Béatrice Niragire Henri Cohen Aminata Kane Anne-Claire Donnadieu Yael Levy-Zauberman Thomas Vernay Juliette Hugueny Etienne Vincens Christine Louis-Sylvestre Agathe Subtil Yongzheng Wu Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
description	Abstract The tumoral origin and extensive passaging of HeLa cells, a most commonly used cervical epithelial cell line, raise concerns on their suitability to study the cell responses to infection. The present study was designed to isolate primary epithelial cells from human ectocervix explants and characterize their susceptibility to C. trachomatis infection. We achieved a high purity of isolation, assessed by the expression of E-cadherin and cytokeratin 14. The infectious progeny in these primary epithelial cells was lower than in HeLa cells. We showed that the difference in culture medium, and the addition of serum in HeLa cultures, accounted for a large part of these differences. However, all things considered the primary ectocervical epithelial cells remained less permissive than HeLa cells to C. trachomatis serovar L2 or D development. Finally, the basal level of transcription of genes coding for pro-inflammatory cytokines was globally higher in primary epithelial cells than in HeLa cells. Transcription of several pro-inflammatory genes was further induced by infection with C. trachomatis serovar L2 or serovar D. In conclusion, primary epithelial cells have a strong capacity to mount an inflammatory response to Chlamydia infection. Our simplified purification protocol from human explants should facilitate future studies to understand the contribution of this response to limiting the spread of the pathogen to the upper female genital tract.
format	article
author	Chongfa Tang Chang Liu Benoit Maffei Béatrice Niragire Henri Cohen Aminata Kane Anne-Claire Donnadieu Yael Levy-Zauberman Thomas Vernay Juliette Hugueny Etienne Vincens Christine Louis-Sylvestre Agathe Subtil Yongzheng Wu
author_facet	Chongfa Tang Chang Liu Benoit Maffei Béatrice Niragire Henri Cohen Aminata Kane Anne-Claire Donnadieu Yael Levy-Zauberman Thomas Vernay Juliette Hugueny Etienne Vincens Christine Louis-Sylvestre Agathe Subtil Yongzheng Wu
author_sort	Chongfa Tang
title	Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
title_short	Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
title_full	Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
title_fullStr	Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
title_full_unstemmed	Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile
title_sort	primary ectocervical epithelial cells display lower permissivity to chlamydia trachomatis than hela cells and a globally higher pro-inflammatory profile
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/195d3f7cbfb64e4db7b039c292bd24fa
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Primary ectocervical epithelial cells display lower permissivity to Chlamydia trachomatis than HeLa cells and a globally higher pro-inflammatory profile

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