Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage

Jae Geun Song, Sang Hoon Lee, Hyo-Kyung Han College of Pharmacy, Dongguk University, Goyang, South Korea Abstract: This study aimed to evaluate aminoclay (3-aminopropyl-functionalized magnesium phyllosilicate) as an effective protectant for the stabilization of protein formulation in freeze-drying...

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Autores principales:	Song JG, Lee SH, Han HK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	organoclay cryoprotectant lyoprotectant freeze-drying protein stability Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1962201fa0ff465391d545c1a0982c64
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spelling	oai:doaj.org-article:1962201fa0ff465391d545c1a0982c642021-12-02T02:38:20ZBiophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage1178-2013https://doaj.org/article/1962201fa0ff465391d545c1a0982c642016-12-01T00:00:00Zhttps://www.dovepress.com/biophysical-evaluation-of-aminoclay-as-an-effective-protectant-for-pr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jae Geun Song, Sang Hoon Lee, Hyo-Kyung Han College of Pharmacy, Dongguk University, Goyang, South Korea Abstract: This study aimed to evaluate aminoclay (3-aminopropyl-functionalized magnesium phyllosilicate) as an effective protectant for the stabilization of protein formulation in freeze-drying. Bovine serum albumin (BSA), as a model protein, was freeze-dried with aminoclay at various concentrations, and the effects of aminoclay on the structural stability of proteins were compared with those of the conventional stabilizers. The structural characteristics of the protein were determined by size exclusion chromatography (SEC), circular dichroism (CD), and Fourier transform infrared (FTIR) spectroscopy. Furthermore, physicochemical and morphological characteristics were examined by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). XRPD and DSC patterns indicated that the glass transition temperature (Tg) of the amorphous formulation of aminoclay mixed with proteins was gradually elevated as the concentration of aminoclay increased. FTIR and CD spectral analysis suggested that the protein structure was well maintained with aminoclay during the freeze-drying process and 3 months of storage at 4°C and 40°C. Furthermore, aminoclay conferred the greatest protection against aggregation and retained the monomer content of BSA even at a high temperature. The morphological characteristics of lyophilized proteins were also well conserved during the storage with aminoclay. These results suggested that aminoclay may be useful as an alternative stabilizer for maintaining the structural stability of protein formulations. Keywords: aminoclay, cryoprotectant, lyoprotectant, freeze-drying, protein, stabilitySong JGLee SHHan HKDove Medical Pressarticleorganoclaycryoprotectantlyoprotectantfreeze-dryingproteinstabilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6609-6619 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	organoclay cryoprotectant lyoprotectant freeze-drying protein stability Medicine (General) R5-920
spellingShingle	organoclay cryoprotectant lyoprotectant freeze-drying protein stability Medicine (General) R5-920 Song JG Lee SH Han HK Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
description	Jae Geun Song, Sang Hoon Lee, Hyo-Kyung Han College of Pharmacy, Dongguk University, Goyang, South Korea Abstract: This study aimed to evaluate aminoclay (3-aminopropyl-functionalized magnesium phyllosilicate) as an effective protectant for the stabilization of protein formulation in freeze-drying. Bovine serum albumin (BSA), as a model protein, was freeze-dried with aminoclay at various concentrations, and the effects of aminoclay on the structural stability of proteins were compared with those of the conventional stabilizers. The structural characteristics of the protein were determined by size exclusion chromatography (SEC), circular dichroism (CD), and Fourier transform infrared (FTIR) spectroscopy. Furthermore, physicochemical and morphological characteristics were examined by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). XRPD and DSC patterns indicated that the glass transition temperature (Tg) of the amorphous formulation of aminoclay mixed with proteins was gradually elevated as the concentration of aminoclay increased. FTIR and CD spectral analysis suggested that the protein structure was well maintained with aminoclay during the freeze-drying process and 3 months of storage at 4°C and 40°C. Furthermore, aminoclay conferred the greatest protection against aggregation and retained the monomer content of BSA even at a high temperature. The morphological characteristics of lyophilized proteins were also well conserved during the storage with aminoclay. These results suggested that aminoclay may be useful as an alternative stabilizer for maintaining the structural stability of protein formulations. Keywords: aminoclay, cryoprotectant, lyoprotectant, freeze-drying, protein, stability
format	article
author	Song JG Lee SH Han HK
author_facet	Song JG Lee SH Han HK
author_sort	Song JG
title	Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
title_short	Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
title_full	Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
title_fullStr	Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
title_full_unstemmed	Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
title_sort	biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/1962201fa0ff465391d545c1a0982c64
work_keys_str_mv	AT songjg biophysicalevaluationofaminoclayasaneffectiveprotectantforproteinstabilizationduringfreezedryingandstorage AT leesh biophysicalevaluationofaminoclayasaneffectiveprotectantforproteinstabilizationduringfreezedryingandstorage AT hanhk biophysicalevaluationofaminoclayasaneffectiveprotectantforproteinstabilizationduringfreezedryingandstorage
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Biophysical evaluation of aminoclay as an effective protectant for protein stabilization during freeze-drying and storage

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