Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent don...

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Autores principales:	Halida Adistya Putri, Agus Purwito, Sudarsono Sudarsono, Dewi Sukma
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	dickeya dadantii, irradiation, phalaenopsis amabilis, pto gene, snap markers, soft rot disease Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1965f2b4fa3740b389d18de902314e7b
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spelling	oai:doaj.org-article:1965f2b4fa3740b389d18de902314e7b2021-11-22T00:56:37ZMorphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms1412-033X2085-472210.13057/biodiv/d220301https://doaj.org/article/1965f2b4fa3740b389d18de902314e7b2021-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7190https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.Halida Adistya PutriAgus PurwitoSudarsono SudarsonoDewi SukmaMBI & UNS Soloarticledickeya dadantii, irradiation, phalaenopsis amabilis, pto gene, snap markers, soft rot diseaseBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dickeya dadantii, irradiation, phalaenopsis amabilis, pto gene, snap markers, soft rot disease Biology (General) QH301-705.5
spellingShingle	dickeya dadantii, irradiation, phalaenopsis amabilis, pto gene, snap markers, soft rot disease Biology (General) QH301-705.5 Halida Adistya Putri Agus Purwito Sudarsono Sudarsono Dewi Sukma Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
description	Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.
format	article
author	Halida Adistya Putri Agus Purwito Sudarsono Sudarsono Dewi Sukma
author_facet	Halida Adistya Putri Agus Purwito Sudarsono Sudarsono Dewi Sukma
author_sort	Halida Adistya Putri
title	Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
title_short	Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
title_full	Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
title_fullStr	Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
title_full_unstemmed	Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms
title_sort	morphological, molecular and resistance responses to soft-rot disease variability among plantlets of phalaenopsis amabilis regenerated from irradiated protocorms
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/1965f2b4fa3740b389d18de902314e7b
work_keys_str_mv	AT halidaadistyaputri morphologicalmolecularandresistanceresponsestosoftrotdiseasevariabilityamongplantletsofphalaenopsisamabilisregeneratedfromirradiatedprotocorms AT aguspurwito morphologicalmolecularandresistanceresponsestosoftrotdiseasevariabilityamongplantletsofphalaenopsisamabilisregeneratedfromirradiatedprotocorms AT sudarsonosudarsono morphologicalmolecularandresistanceresponsestosoftrotdiseasevariabilityamongplantletsofphalaenopsisamabilisregeneratedfromirradiatedprotocorms AT dewisukma morphologicalmolecularandresistanceresponsestosoftrotdiseasevariabilityamongplantletsofphalaenopsisamabilisregeneratedfromirradiatedprotocorms
_version_	1718418385335746560

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

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