Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential is...

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Autores principales:	MAZYTHA KINANTI RACHMANIA, FITRIA NINGSIH, PUTRI PRATIWI SETYANINGSIH, WINDA AYU SYAFITRI, DHIAN CHITRA AYU FITRIA SARI, SHUHEI YABE, AKIRA YOKOTA, ARIYANTI OETARI, Wellyzar Sjamsuridzal
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cisolok geysers, soil, thermophilic actinobacteria, xylanase Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1975a5e0390540fbb5db661f12fcb4c8
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spelling	oai:doaj.org-article:1975a5e0390540fbb5db661f12fcb4c82021-11-21T22:09:25ZXylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area1412-033X2085-472210.13057/biodiv/d210119https://doaj.org/article/1975a5e0390540fbb5db661f12fcb4c82019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4656https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.MAZYTHA KINANTI RACHMANIAFITRIA NINGSIHPUTRI PRATIWI SETYANINGSIHWINDA AYU SYAFITRIDHIAN CHITRA AYU FITRIA SARISHUHEI YABEAKIRA YOKOTAARIYANTI OETARIWellyzar SjamsuridzalMBI & UNS Soloarticlecisolok geysers, soil, thermophilic actinobacteria, xylanaseBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cisolok geysers, soil, thermophilic actinobacteria, xylanase Biology (General) QH301-705.5
spellingShingle	cisolok geysers, soil, thermophilic actinobacteria, xylanase Biology (General) QH301-705.5 MAZYTHA KINANTI RACHMANIA FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
description	Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.
format	article
author	MAZYTHA KINANTI RACHMANIA FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal
author_facet	MAZYTHA KINANTI RACHMANIA FITRIA NINGSIH PUTRI PRATIWI SETYANINGSIH WINDA AYU SYAFITRI DHIAN CHITRA AYU FITRIA SARI SHUHEI YABE AKIRA YOKOTA ARIYANTI OETARI Wellyzar Sjamsuridzal
author_sort	MAZYTHA KINANTI RACHMANIA
title	Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
title_short	Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
title_full	Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
title_fullStr	Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
title_full_unstemmed	Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area
title_sort	xylan-degrading ability of thermophilic actinobacteria from soil in a geothermal area
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/1975a5e0390540fbb5db661f12fcb4c8
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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

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