<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells

Tissue remodeling contributes to ongoing inflammation and refractoriness of chronic rhinosinusitis (CRS). During this process, epithelial-mesenchymal transition (EMT) plays an important role in dysregulated remodeling and both <i>microRNA (miR)-29b</i> and heat shock protein 47 (HSP47) m...

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Autores principales:	Jae-Min Shin, Joo-Hoo Park, Hyun-Woo Yang, Jee Won Moon, Heung-Man Lee, Il-Ho Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microRNA heat shock protein 47 epithelial–mesenchymal transition transforming growth factor beta-1 tissue remodeling primary nasal epithelial cells Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/19782d895def4032b7cd9359a14060fc
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spelling	oai:doaj.org-article:19782d895def4032b7cd9359a14060fc2021-11-11T17:00:05Z<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells10.3390/ijms2221115351422-00671661-6596https://doaj.org/article/19782d895def4032b7cd9359a14060fc2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11535https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Tissue remodeling contributes to ongoing inflammation and refractoriness of chronic rhinosinusitis (CRS). During this process, epithelial-mesenchymal transition (EMT) plays an important role in dysregulated remodeling and both <i>microRNA (miR)-29b</i> and heat shock protein 47 (HSP47) may be engaged in the pathophysiology of CRS. This study aimed to determine the role of <i>miR-29b</i> and HSP47 in modulating transforming growth factor (TGF)-β1-induced EMT and migration in airway epithelial cells. Expression levels of <i>miR-29b</i>, HSP47, E-cadherin, α-smooth muscle actin (α-SMA), vimentin and fibronectin were assessed through real-time PCR, Western blotting, and immunofluorescence staining. <i>Small interfering RNA</i> (<i>siRNA</i>) targeted against <i>miR-29b</i> and <i>HSP47</i> were transfected to regulate the expression of EMT-related markers. Cell migration was evaluated with wound scratch and transwell migration assay. <i>miR-29b</i> mimic significantly inhibited the expression of HSP47 and TGF-β1-induced EMT-related markers in A549 cells. However, the <i>miR-29b</i> inhibitor more greatly induced the expression of them. HSP47 knockout suppressed TGF-β1-induced EMT marker levels. Functional studies indicated that TGF-β1-induced EMT was regulated by <i>miR-29b</i> and HSP47 in A549 cells. These findings were further verified in primary nasal epithelial cells. <i>miR-29b</i> modulated TGF-β1-induced EMT-related markers and migration via HSP47 expression modulation in A549 and primary nasal epithelial cells. These results suggested the importance of <i>miR-29b</i> and HSP47 in pathologic tissue remodeling progression in CRS.Jae-Min ShinJoo-Hoo ParkHyun-Woo YangJee Won MoonHeung-Man LeeIl-Ho ParkMDPI AGarticlemicroRNAheat shock protein 47epithelial–mesenchymal transitiontransforming growth factor beta-1tissue remodelingprimary nasal epithelial cellsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11535, p 11535 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microRNA heat shock protein 47 epithelial–mesenchymal transition transforming growth factor beta-1 tissue remodeling primary nasal epithelial cells Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	microRNA heat shock protein 47 epithelial–mesenchymal transition transforming growth factor beta-1 tissue remodeling primary nasal epithelial cells Biology (General) QH301-705.5 Chemistry QD1-999 Jae-Min Shin Joo-Hoo Park Hyun-Woo Yang Jee Won Moon Heung-Man Lee Il-Ho Park <i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
description	Tissue remodeling contributes to ongoing inflammation and refractoriness of chronic rhinosinusitis (CRS). During this process, epithelial-mesenchymal transition (EMT) plays an important role in dysregulated remodeling and both <i>microRNA (miR)-29b</i> and heat shock protein 47 (HSP47) may be engaged in the pathophysiology of CRS. This study aimed to determine the role of <i>miR-29b</i> and HSP47 in modulating transforming growth factor (TGF)-β1-induced EMT and migration in airway epithelial cells. Expression levels of <i>miR-29b</i>, HSP47, E-cadherin, α-smooth muscle actin (α-SMA), vimentin and fibronectin were assessed through real-time PCR, Western blotting, and immunofluorescence staining. <i>Small interfering RNA</i> (<i>siRNA</i>) targeted against <i>miR-29b</i> and <i>HSP47</i> were transfected to regulate the expression of EMT-related markers. Cell migration was evaluated with wound scratch and transwell migration assay. <i>miR-29b</i> mimic significantly inhibited the expression of HSP47 and TGF-β1-induced EMT-related markers in A549 cells. However, the <i>miR-29b</i> inhibitor more greatly induced the expression of them. HSP47 knockout suppressed TGF-β1-induced EMT marker levels. Functional studies indicated that TGF-β1-induced EMT was regulated by <i>miR-29b</i> and HSP47 in A549 cells. These findings were further verified in primary nasal epithelial cells. <i>miR-29b</i> modulated TGF-β1-induced EMT-related markers and migration via HSP47 expression modulation in A549 and primary nasal epithelial cells. These results suggested the importance of <i>miR-29b</i> and HSP47 in pathologic tissue remodeling progression in CRS.
format	article
author	Jae-Min Shin Joo-Hoo Park Hyun-Woo Yang Jee Won Moon Heung-Man Lee Il-Ho Park
author_facet	Jae-Min Shin Joo-Hoo Park Hyun-Woo Yang Jee Won Moon Heung-Man Lee Il-Ho Park
author_sort	Jae-Min Shin
title	<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
title_short	<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
title_full	<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
title_fullStr	<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
title_full_unstemmed	<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
title_sort	<i>mir-29b</i> regulates tgf-β1-induced epithelial–mesenchymal transition by inhibiting heat shock protein 47 expression in airway epithelial cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/19782d895def4032b7cd9359a14060fc
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<i>miR-29b</i> Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells

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