Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil

Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established...

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Autores principales:	Nicholas Akomeng, Stephen Adusei
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Soil Spectrophotometry Total phenolic content Organic solvents Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/19805daecfec4d5b9089faac93ecf6ff
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spelling	oai:doaj.org-article:19805daecfec4d5b9089faac93ecf6ff2021-12-02T05:02:58ZOrganic solvent extraction and spectrophotometric quantification of total phenolic content of soil2405-844010.1016/j.heliyon.2021.e08388https://doaj.org/article/19805daecfec4d5b9089faac93ecf6ff2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024919https://doaj.org/toc/2405-8440Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established that phenolic compounds can influence nutrients availability and soil quality, it becomes crucial to investigate into soil phenolics through the application of appropriate extraction technique and quantification of total phenolic content in soils. This study therefore aimed at utilizing ethanol, hexane and diethyl ether organic solvents to extract and quantify total phenolic content of soil, sampled from a vegetable growing area. Conventional organic solvent extraction method was employed to extract phenolics, while spectrophotometric technique was utilized to quantify total phenolic content. The highest extraction yield of 34.52% was achieved with ethanol followed by diethyl ether (28.23%) and hexane (25.47%). Interestingly, hexane, which had the least extraction yield, rather recorded the highest phenolics concentration of 5.50 ± 0.02 mgGAE/g, with ethanol producing a concentration of 2.04 ± 0.05 mgGAE/g and 3.82 ± 0.01 mgGAE/g for diethyl ether. The percent recovery, limit of detection (LOD) and limit of quantification (LOQ) of phenolic compounds were found to be 102%, 0.8 mg/g and 1.5 mg/g for ethanol; 96%, 0.6 mg/g and 1.2 mg/g for diethyl ether and 94%, 0.4 mg/g and 1.0 mg/g for hexane respectively. These results indicate that for an extraction efficiency and greater yield, the use of ethanol as solvent is preferred whereas extraction using hexane is suitable for total phenolics quantification. The findings of this study have provided a vital insight regarding the influence of organic solvents on the extractability and quantification of total phenolic content of soil.Nicholas AkomengStephen AduseiElsevierarticleSoilSpectrophotometryTotal phenolic contentOrganic solventsScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08388- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Soil Spectrophotometry Total phenolic content Organic solvents Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Soil Spectrophotometry Total phenolic content Organic solvents Science (General) Q1-390 Social sciences (General) H1-99 Nicholas Akomeng Stephen Adusei Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
description	Phenolic compounds are regarded as the most abundant plant metabolites that are known to decompose progressively into soils, likened to other soil organic materials. Once assimilated into soils, they can control soil processes, including organic matter decomposition and nutrient cycling. Established that phenolic compounds can influence nutrients availability and soil quality, it becomes crucial to investigate into soil phenolics through the application of appropriate extraction technique and quantification of total phenolic content in soils. This study therefore aimed at utilizing ethanol, hexane and diethyl ether organic solvents to extract and quantify total phenolic content of soil, sampled from a vegetable growing area. Conventional organic solvent extraction method was employed to extract phenolics, while spectrophotometric technique was utilized to quantify total phenolic content. The highest extraction yield of 34.52% was achieved with ethanol followed by diethyl ether (28.23%) and hexane (25.47%). Interestingly, hexane, which had the least extraction yield, rather recorded the highest phenolics concentration of 5.50 ± 0.02 mgGAE/g, with ethanol producing a concentration of 2.04 ± 0.05 mgGAE/g and 3.82 ± 0.01 mgGAE/g for diethyl ether. The percent recovery, limit of detection (LOD) and limit of quantification (LOQ) of phenolic compounds were found to be 102%, 0.8 mg/g and 1.5 mg/g for ethanol; 96%, 0.6 mg/g and 1.2 mg/g for diethyl ether and 94%, 0.4 mg/g and 1.0 mg/g for hexane respectively. These results indicate that for an extraction efficiency and greater yield, the use of ethanol as solvent is preferred whereas extraction using hexane is suitable for total phenolics quantification. The findings of this study have provided a vital insight regarding the influence of organic solvents on the extractability and quantification of total phenolic content of soil.
format	article
author	Nicholas Akomeng Stephen Adusei
author_facet	Nicholas Akomeng Stephen Adusei
author_sort	Nicholas Akomeng
title	Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_short	Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_full	Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_fullStr	Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_full_unstemmed	Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
title_sort	organic solvent extraction and spectrophotometric quantification of total phenolic content of soil
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/19805daecfec4d5b9089faac93ecf6ff
work_keys_str_mv	AT nicholasakomeng organicsolventextractionandspectrophotometricquantificationoftotalphenoliccontentofsoil AT stephenadusei organicsolventextractionandspectrophotometricquantificationoftotalphenoliccontentofsoil
_version_	1718400694983065600

Organic solvent extraction and spectrophotometric quantification of total phenolic content of soil

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