Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.

Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities...

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Autores principales:	Stephen A Jackson, Burkhardt Flemer, Angela McCann, Jonathan Kennedy, John P Morrissey, Fergal O'Gara, Alan D W Dobson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1981423fae014aeeb9d3f24f47de5f01
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spelling	oai:doaj.org-article:1981423fae014aeeb9d3f24f47de5f012021-11-18T08:39:47ZArchaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.1932-620310.1371/journal.pone.0084438https://doaj.org/article/1981423fae014aeeb9d3f24f47de5f012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386380/?tool=EBIhttps://doaj.org/toc/1932-6203Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities in sponges and have involved sponges sampled from shallow waters. Here, we used pyrosequencing of 16S rRNA genes to compare the bacterial and archaeal communities associated with two individuals of the marine sponge Inflatella pellicula from the deep-sea, sampled from a depth of 2,900 m, a depth which far exceeds any previous sequence-based report of sponge-associated microbial communities. Sponge-microbial communities were also compared to the microbial community in the surrounding seawater. Sponge-associated microbial communities were dominated by archaeal sequencing reads with a single archaeal OTU, comprising ~60% and ~72% of sequences, being observed from Inflatella pellicula. Archaeal sequencing reads were less abundant in seawater (~11% of sequences). Sponge-associated microbial communities were less diverse and less even than any other sponge-microbial community investigated to date with just 210 and 273 OTUs (97% sequence identity) identified in sponges, with 4 and 6 dominant OTUs comprising ~88% and ~89% of sequences, respectively. Members of the candidate phyla, SAR406, NC10 and ZB3 are reported here from sponges for the first time, increasing the number of bacterial phyla or candidate divisions associated with sponges to 43. A minor cohort from both sponge samples (~0.2% and ~0.3% of sequences) were not classified to phylum level. A single OTU, common to both sponge individuals, dominates these unclassified reads and shares sequence homology with a sponge associated clone which itself has no known close relative and may represent a novel taxon.Stephen A JacksonBurkhardt FlemerAngela McCannJonathan KennedyJohn P MorrisseyFergal O'GaraAlan D W DobsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84438 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen A Jackson Burkhardt Flemer Angela McCann Jonathan Kennedy John P Morrissey Fergal O'Gara Alan D W Dobson Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
description	Microbes associated with marine sponges play significant roles in host physiology. Remarkable levels of microbial diversity have been observed in sponges worldwide through both culture-dependent and culture-independent studies. Most studies have focused on the structure of the bacterial communities in sponges and have involved sponges sampled from shallow waters. Here, we used pyrosequencing of 16S rRNA genes to compare the bacterial and archaeal communities associated with two individuals of the marine sponge Inflatella pellicula from the deep-sea, sampled from a depth of 2,900 m, a depth which far exceeds any previous sequence-based report of sponge-associated microbial communities. Sponge-microbial communities were also compared to the microbial community in the surrounding seawater. Sponge-associated microbial communities were dominated by archaeal sequencing reads with a single archaeal OTU, comprising ~60% and ~72% of sequences, being observed from Inflatella pellicula. Archaeal sequencing reads were less abundant in seawater (~11% of sequences). Sponge-associated microbial communities were less diverse and less even than any other sponge-microbial community investigated to date with just 210 and 273 OTUs (97% sequence identity) identified in sponges, with 4 and 6 dominant OTUs comprising ~88% and ~89% of sequences, respectively. Members of the candidate phyla, SAR406, NC10 and ZB3 are reported here from sponges for the first time, increasing the number of bacterial phyla or candidate divisions associated with sponges to 43. A minor cohort from both sponge samples (~0.2% and ~0.3% of sequences) were not classified to phylum level. A single OTU, common to both sponge individuals, dominates these unclassified reads and shares sequence homology with a sponge associated clone which itself has no known close relative and may represent a novel taxon.
format	article
author	Stephen A Jackson Burkhardt Flemer Angela McCann Jonathan Kennedy John P Morrissey Fergal O'Gara Alan D W Dobson
author_facet	Stephen A Jackson Burkhardt Flemer Angela McCann Jonathan Kennedy John P Morrissey Fergal O'Gara Alan D W Dobson
author_sort	Stephen A Jackson
title	Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
title_short	Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
title_full	Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
title_fullStr	Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
title_full_unstemmed	Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.
title_sort	archaea appear to dominate the microbiome of inflatella pellicula deep sea sponges.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1981423fae014aeeb9d3f24f47de5f01
work_keys_str_mv	AT stephenajackson archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT burkhardtflemer archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT angelamccann archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT jonathankennedy archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT johnpmorrissey archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT fergalogara archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges AT alandwdobson archaeaappeartodominatethemicrobiomeofinflatellapelliculadeepseasponges
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Archaea appear to dominate the microbiome of Inflatella pellicula deep sea sponges.

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