CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung

The transcription factor NKX2-1 is a key regulator of lung pathophysiology, but the importance of its binding sites outside of proximal promoter regions is unclear. Here, William Stuart and Iris Fink-Baldauf et al. use CRISPRi to interrogate 19 NKX2-1 binding sites and identify specific sites import...

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Autores principales:	William D. Stuart, Iris M. Fink-Baldauf, Koichi Tomoshige, Minzhe Guo, Yutaka Maeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/198178427e5643a7a1bfc5472c5d09ff
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spelling	oai:doaj.org-article:198178427e5643a7a1bfc5472c5d09ff2021-12-02T15:43:23ZCRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung10.1038/s42003-021-02083-42399-3642https://doaj.org/article/198178427e5643a7a1bfc5472c5d09ff2021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02083-4https://doaj.org/toc/2399-3642The transcription factor NKX2-1 is a key regulator of lung pathophysiology, but the importance of its binding sites outside of proximal promoter regions is unclear. Here, William Stuart and Iris Fink-Baldauf et al. use CRISPRi to interrogate 19 NKX2-1 binding sites and identify specific sites important for breathing, innate immune defense, and tumorigenesis.William D. StuartIris M. Fink-BaldaufKoichi TomoshigeMinzhe GuoYutaka MaedaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 William D. Stuart Iris M. Fink-Baldauf Koichi Tomoshige Minzhe Guo Yutaka Maeda CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
description	The transcription factor NKX2-1 is a key regulator of lung pathophysiology, but the importance of its binding sites outside of proximal promoter regions is unclear. Here, William Stuart and Iris Fink-Baldauf et al. use CRISPRi to interrogate 19 NKX2-1 binding sites and identify specific sites important for breathing, innate immune defense, and tumorigenesis.
format	article
author	William D. Stuart Iris M. Fink-Baldauf Koichi Tomoshige Minzhe Guo Yutaka Maeda
author_facet	William D. Stuart Iris M. Fink-Baldauf Koichi Tomoshige Minzhe Guo Yutaka Maeda
author_sort	William D. Stuart
title	CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
title_short	CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
title_full	CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
title_fullStr	CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
title_full_unstemmed	CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung
title_sort	crispri-mediated functional analysis of nkx2-1-binding sites in the lung
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/198178427e5643a7a1bfc5472c5d09ff
work_keys_str_mv	AT williamdstuart crisprimediatedfunctionalanalysisofnkx21bindingsitesinthelung AT irismfinkbaldauf crisprimediatedfunctionalanalysisofnkx21bindingsitesinthelung AT koichitomoshige crisprimediatedfunctionalanalysisofnkx21bindingsitesinthelung AT minzheguo crisprimediatedfunctionalanalysisofnkx21bindingsitesinthelung AT yutakamaeda crisprimediatedfunctionalanalysisofnkx21bindingsitesinthelung
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CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung

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