Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.

The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could...

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Autores principales:	Shulei Qiu, Jin Chen, Tao Qin, Yuanliang Hu, Deyun Wang, Qiang Fan, Cunshuai Zhang, Xingying Chen, Xiaolan Chen, Cui Liu, Zhenzhen Gao, Xiuping Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19833ba1c1ac4e309de4ceb4ae32095c
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spelling	oai:doaj.org-article:19833ba1c1ac4e309de4ceb4ae32095c2021-11-18T08:34:54ZEffects of selenylation modification on immune-enhancing activity of garlic polysaccharide.1932-620310.1371/journal.pone.0086377https://doaj.org/article/19833ba1c1ac4e309de4ceb4ae32095c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24497946/?tool=EBIhttps://doaj.org/toc/1932-6203The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could significantly promote lymphocytes proliferation, sGPS3, sGPS5 and sGPS6 presented stronger efficacy. In vivo experiment, 14-day-old chickens were injected respectively with sGPS3, sGPS5 and sGPS6 when they were vaccinated with ND vaccine taking unmodified GPS as control. The results showed that three sGPSs could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-γ and IL-2 contents. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of GPS, sGPS6 possessed the best efficacy and could be as a candidate drug of immunoenhancer. Its optimal modification conditions were 400 mg of sodium selenite for 500 mg of GPS, reaction temperature of 70°C and reaction time of 6 h.Shulei QiuJin ChenTao QinYuanliang HuDeyun WangQiang FanCunshuai ZhangXingying ChenXiaolan ChenCui LiuZhenzhen GaoXiuping LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86377 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shulei Qiu Jin Chen Tao Qin Yuanliang Hu Deyun Wang Qiang Fan Cunshuai Zhang Xingying Chen Xiaolan Chen Cui Liu Zhenzhen Gao Xiuping Li Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
description	The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could significantly promote lymphocytes proliferation, sGPS3, sGPS5 and sGPS6 presented stronger efficacy. In vivo experiment, 14-day-old chickens were injected respectively with sGPS3, sGPS5 and sGPS6 when they were vaccinated with ND vaccine taking unmodified GPS as control. The results showed that three sGPSs could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-γ and IL-2 contents. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of GPS, sGPS6 possessed the best efficacy and could be as a candidate drug of immunoenhancer. Its optimal modification conditions were 400 mg of sodium selenite for 500 mg of GPS, reaction temperature of 70°C and reaction time of 6 h.
format	article
author	Shulei Qiu Jin Chen Tao Qin Yuanliang Hu Deyun Wang Qiang Fan Cunshuai Zhang Xingying Chen Xiaolan Chen Cui Liu Zhenzhen Gao Xiuping Li
author_facet	Shulei Qiu Jin Chen Tao Qin Yuanliang Hu Deyun Wang Qiang Fan Cunshuai Zhang Xingying Chen Xiaolan Chen Cui Liu Zhenzhen Gao Xiuping Li
author_sort	Shulei Qiu
title	Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
title_short	Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
title_full	Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
title_fullStr	Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
title_full_unstemmed	Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
title_sort	effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/19833ba1c1ac4e309de4ceb4ae32095c
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Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide.

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