Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC

The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regar...

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Autores principales:	Anca Becze, Vanda Liliana Babalau Fuss, Daniela Alexandra Scurtu, Maria Tomoaia-Cotisel, Aurora Mocanu, Oana Cadar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	UHPLC method validation calcitriol cholecalciferol menaquinone-4 menaquinone-7 Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/1983501203e648f09671090a9edd00b9
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spelling	oai:doaj.org-article:1983501203e648f09671090a9edd00b92021-11-25T18:28:54ZSimultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC10.3390/molecules262269821420-3049https://doaj.org/article/1983501203e648f09671090a9edd00b92021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6982https://doaj.org/toc/1420-3049The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regard, the simultaneous determination of vitamin D3 (calcitriol—CT and cholecalciferol—CHL) and K2 (menaquinone-4—MK-4 and menaquinone-7—MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04–0.05 µg/mL, respectively. The validation results indicated that the method had reasonable linearity (<i>R</i><sup>2</sup> ≥ 0.9990), good recovery (>82%), satisfactory intra-day precision (≤1.9%) and inter-day precision (≤3.5%), and high selectivity and specificity. The validated UHPLC method was demonstrated to be precise, accurate, and robust for the simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in dietary supplements.Anca BeczeVanda Liliana Babalau FussDaniela Alexandra ScurtuMaria Tomoaia-CotiselAurora MocanuOana CadarMDPI AGarticleUHPLCmethod validationcalcitriolcholecalciferolmenaquinone-4menaquinone-7Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6982, p 6982 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	UHPLC method validation calcitriol cholecalciferol menaquinone-4 menaquinone-7 Organic chemistry QD241-441
spellingShingle	UHPLC method validation calcitriol cholecalciferol menaquinone-4 menaquinone-7 Organic chemistry QD241-441 Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
description	The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regard, the simultaneous determination of vitamin D3 (calcitriol—CT and cholecalciferol—CHL) and K2 (menaquinone-4—MK-4 and menaquinone-7—MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04–0.05 µg/mL, respectively. The validation results indicated that the method had reasonable linearity (<i>R</i><sup>2</sup> ≥ 0.9990), good recovery (>82%), satisfactory intra-day precision (≤1.9%) and inter-day precision (≤3.5%), and high selectivity and specificity. The validated UHPLC method was demonstrated to be precise, accurate, and robust for the simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in dietary supplements.
format	article
author	Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar
author_facet	Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar
author_sort	Anca Becze
title	Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
title_short	Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
title_full	Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
title_fullStr	Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
title_full_unstemmed	Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
title_sort	simultaneous determination of vitamins d3 (calcitriol, cholecalciferol) and k2 (menaquinone-4 and menaquinone-7) in dietary supplements by uhplc
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1983501203e648f09671090a9edd00b9
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Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC

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