Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex

Abstract The end of the critical period for primary visual cortex (V1) coincides with the deposition of perineuronal nets (PNN) onto Parvalbumin (PV) inhibitory neurons. Recently, we found that transplantation of embryonic inhibitory neurons into adult V1 reinstates a new critical period. Here we us...

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Autores principales:	Karen P. Bradshaw, Dario X. Figueroa Velez, Mariyam Habeeb, Sunil P. Gandhi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/19871b620d2c46a8a1ded10ed8c4e494
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spelling	oai:doaj.org-article:19871b620d2c46a8a1ded10ed8c4e4942021-12-02T16:08:02ZPrecocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex10.1038/s41598-018-25735-82045-2322https://doaj.org/article/19871b620d2c46a8a1ded10ed8c4e4942018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25735-8https://doaj.org/toc/2045-2322Abstract The end of the critical period for primary visual cortex (V1) coincides with the deposition of perineuronal nets (PNN) onto Parvalbumin (PV) inhibitory neurons. Recently, we found that transplantation of embryonic inhibitory neurons into adult V1 reinstates a new critical period. Here we used Wisteria Floribunda Agglutinin (WFA) staining to compare the deposition of PNNs onto neurons during normal development and following transplantation at equivalent cell ages. In accord with previous findings, PV and PNN expression increases from negligible levels at postnatal day 14 (P14) to mature levels by P70. In contrast to P14, PNNs are found on transplanted PV neurons by 21 days after transplantation and persist to 105 days after transplantation. This precocious deposition was specific to PV neurons and excluded transplanted neurons expressing Somatostatin. Notably, the onset of PV expression in transplanted inhibitory neurons follows the timing of PV expression in juvenile V1. Moreover, transplantation has no discernible effect on host PNNs. The precocious deposition of PNNs onto transplanted PV neurons suggests that PNN expression identified by WFA does not reflect neuronal maturity and may be an inaccurate marker for transplant-induced plasticity of cortical circuits.Karen P. BradshawDario X. Figueroa VelezMariyam HabeebSunil P. GandhiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen P. Bradshaw Dario X. Figueroa Velez Mariyam Habeeb Sunil P. Gandhi Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
description	Abstract The end of the critical period for primary visual cortex (V1) coincides with the deposition of perineuronal nets (PNN) onto Parvalbumin (PV) inhibitory neurons. Recently, we found that transplantation of embryonic inhibitory neurons into adult V1 reinstates a new critical period. Here we used Wisteria Floribunda Agglutinin (WFA) staining to compare the deposition of PNNs onto neurons during normal development and following transplantation at equivalent cell ages. In accord with previous findings, PV and PNN expression increases from negligible levels at postnatal day 14 (P14) to mature levels by P70. In contrast to P14, PNNs are found on transplanted PV neurons by 21 days after transplantation and persist to 105 days after transplantation. This precocious deposition was specific to PV neurons and excluded transplanted neurons expressing Somatostatin. Notably, the onset of PV expression in transplanted inhibitory neurons follows the timing of PV expression in juvenile V1. Moreover, transplantation has no discernible effect on host PNNs. The precocious deposition of PNNs onto transplanted PV neurons suggests that PNN expression identified by WFA does not reflect neuronal maturity and may be an inaccurate marker for transplant-induced plasticity of cortical circuits.
format	article
author	Karen P. Bradshaw Dario X. Figueroa Velez Mariyam Habeeb Sunil P. Gandhi
author_facet	Karen P. Bradshaw Dario X. Figueroa Velez Mariyam Habeeb Sunil P. Gandhi
author_sort	Karen P. Bradshaw
title	Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
title_short	Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
title_full	Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
title_fullStr	Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
title_full_unstemmed	Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex
title_sort	precocious deposition of perineuronal nets on parvalbumin inhibitory neurons transplanted into adult visual cortex
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/19871b620d2c46a8a1ded10ed8c4e494
work_keys_str_mv	AT karenpbradshaw precociousdepositionofperineuronalnetsonparvalbumininhibitoryneuronstransplantedintoadultvisualcortex AT darioxfigueroavelez precociousdepositionofperineuronalnetsonparvalbumininhibitoryneuronstransplantedintoadultvisualcortex AT mariyamhabeeb precociousdepositionofperineuronalnetsonparvalbumininhibitoryneuronstransplantedintoadultvisualcortex AT sunilpgandhi precociousdepositionofperineuronalnetsonparvalbumininhibitoryneuronstransplantedintoadultvisualcortex
_version_	1718384641281359872

Precocious deposition of perineuronal nets on Parvalbumin inhibitory neurons transplanted into adult visual cortex

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