Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients

To characterize metabolites and metabolic pathways altered in intermediate and neovascular age-related macular degeneration (IAMD and NVAMD), high resolution untargeted metabolomics was performed via liquid chromatography-mass spectrometry on plasma samples obtained from 91 IAMD patients, 100 NVAMD...

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Autores principales:	Sabrina L. Mitchell, Chunyu Ma, William K. Scott, Anita Agarwal, Margaret A. Pericak-Vance, Jonathan L. Haines, Dean P. Jones, Karan Uppal, Milam A. Brantley
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	age-related macular degeneration IAMD NVAMD metabolomics acylcarnitines carnitine shuttle Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1988585a959e449fb6547ea6a03ac1c2
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spelling	oai:doaj.org-article:1988585a959e449fb6547ea6a03ac1c22021-11-25T17:11:54ZPlasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients10.3390/cells101131412073-4409https://doaj.org/article/1988585a959e449fb6547ea6a03ac1c22021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3141https://doaj.org/toc/2073-4409To characterize metabolites and metabolic pathways altered in intermediate and neovascular age-related macular degeneration (IAMD and NVAMD), high resolution untargeted metabolomics was performed via liquid chromatography-mass spectrometry on plasma samples obtained from 91 IAMD patients, 100 NVAMD patients, and 195 controls. Plasma metabolite levels were compared between: AMD patients and controls, IAMD patients and controls, and NVAMD and IAMD patients. Partial least-squares discriminant analysis and linear regression were used to identify discriminatory metabolites. Pathway analysis was performed to determine metabolic pathways altered in AMD. Among the comparisons, we identified 435 unique discriminatory metabolic features. Using computational methods and tandem mass spectrometry, we identified 11 metabolic features whose molecular identities had been previously verified and confirmed the molecular identities of three additional discriminatory features. Included among the discriminatory metabolites were acylcarnitines, phospholipids, amino acids, and steroid metabolites. Pathway analysis revealed that lipid, amino acid, and vitamin metabolism pathways were altered in NVAMD, IAMD, or AMD in general, including the <i>carnitine shuttle</i> pathway which was significantly altered in all comparisons. Finally, few discriminatory features were identified between IAMD patients and controls, suggesting that plasma metabolic profiles of IAMD patients are more similar to controls than to NVAMD patients.Sabrina L. MitchellChunyu MaWilliam K. ScottAnita AgarwalMargaret A. Pericak-VanceJonathan L. HainesDean P. JonesKaran UppalMilam A. BrantleyMDPI AGarticleage-related macular degenerationIAMDNVAMDmetabolomicsacylcarnitinescarnitine shuttleBiology (General)QH301-705.5ENCells, Vol 10, Iss 3141, p 3141 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	age-related macular degeneration IAMD NVAMD metabolomics acylcarnitines carnitine shuttle Biology (General) QH301-705.5
spellingShingle	age-related macular degeneration IAMD NVAMD metabolomics acylcarnitines carnitine shuttle Biology (General) QH301-705.5 Sabrina L. Mitchell Chunyu Ma William K. Scott Anita Agarwal Margaret A. Pericak-Vance Jonathan L. Haines Dean P. Jones Karan Uppal Milam A. Brantley Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
description	To characterize metabolites and metabolic pathways altered in intermediate and neovascular age-related macular degeneration (IAMD and NVAMD), high resolution untargeted metabolomics was performed via liquid chromatography-mass spectrometry on plasma samples obtained from 91 IAMD patients, 100 NVAMD patients, and 195 controls. Plasma metabolite levels were compared between: AMD patients and controls, IAMD patients and controls, and NVAMD and IAMD patients. Partial least-squares discriminant analysis and linear regression were used to identify discriminatory metabolites. Pathway analysis was performed to determine metabolic pathways altered in AMD. Among the comparisons, we identified 435 unique discriminatory metabolic features. Using computational methods and tandem mass spectrometry, we identified 11 metabolic features whose molecular identities had been previously verified and confirmed the molecular identities of three additional discriminatory features. Included among the discriminatory metabolites were acylcarnitines, phospholipids, amino acids, and steroid metabolites. Pathway analysis revealed that lipid, amino acid, and vitamin metabolism pathways were altered in NVAMD, IAMD, or AMD in general, including the <i>carnitine shuttle</i> pathway which was significantly altered in all comparisons. Finally, few discriminatory features were identified between IAMD patients and controls, suggesting that plasma metabolic profiles of IAMD patients are more similar to controls than to NVAMD patients.
format	article
author	Sabrina L. Mitchell Chunyu Ma William K. Scott Anita Agarwal Margaret A. Pericak-Vance Jonathan L. Haines Dean P. Jones Karan Uppal Milam A. Brantley
author_facet	Sabrina L. Mitchell Chunyu Ma William K. Scott Anita Agarwal Margaret A. Pericak-Vance Jonathan L. Haines Dean P. Jones Karan Uppal Milam A. Brantley
author_sort	Sabrina L. Mitchell
title	Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
title_short	Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
title_full	Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
title_fullStr	Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
title_full_unstemmed	Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients
title_sort	plasma metabolomics of intermediate and neovascular age-related macular degeneration patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1988585a959e449fb6547ea6a03ac1c2
work_keys_str_mv	AT sabrinalmitchell plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT chunyuma plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT williamkscott plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT anitaagarwal plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT margaretapericakvance plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT jonathanlhaines plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT deanpjones plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT karanuppal plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients AT milamabrantley plasmametabolomicsofintermediateandneovascularagerelatedmaculardegenerationpatients
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Plasma Metabolomics of Intermediate and Neovascular Age-Related Macular Degeneration Patients

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