Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress

Gossypium barbadense is a cultivated cotton not only known for producing superior fiber but also for its salt and alkaline resistance. Here, we used Whole Genome Bisulfite Sequencing (WGBS) technology to map the cytosine methylation of the whole genome of the G. barbadense hypocotyl at single base r...

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Autores principales:	Cun Rui, Yuexin Zhang, Yapeng Fan, Mingge Han, Maohua Dai, Qinqin Wang, Xiugui Chen, Xuke Lu, Delong Wang, Shuai Wang, Wenwei Gao, John Z. Yu, Wuwei Ye
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Gossypium barbadense cell elongation DNA methylation high pH alkaline responding Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/198a996e6ff4431097bd34dddb8fa380
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spelling	oai:doaj.org-article:198a996e6ff4431097bd34dddb8fa3802021-11-11T14:26:33ZInsight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress1664-462X10.3389/fpls.2021.772123https://doaj.org/article/198a996e6ff4431097bd34dddb8fa3802021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.772123/fullhttps://doaj.org/toc/1664-462XGossypium barbadense is a cultivated cotton not only known for producing superior fiber but also for its salt and alkaline resistance. Here, we used Whole Genome Bisulfite Sequencing (WGBS) technology to map the cytosine methylation of the whole genome of the G. barbadense hypocotyl at single base resolution. The methylation sequencing results showed that the mapping rates of the three samples were 75.32, 77.54, and 77.94%, respectively. In addition, the Bisulfite Sequence (BS) conversion rate was 99.78%. Approximately 71.03, 53.87, and 6.26% of the cytosine were methylated at CG, CHG, and CHH sequence contexts, respectively. A comprehensive analysis of DNA methylation and transcriptome data showed that the methylation level of the promoter region was a positive correlation in the CHH context. Saline-alkaline stress was related to the methylation changes of many genes, transcription factors (TFs) and transposable elements (TEs), respectively. We explored the regulatory mechanism of DNA methylation in response to salt and alkaline stress during cotton hypocotyl elongation. Our data shed light into the relationship of methylation regulation at the germination stage of G. barbadense hypocotyl cell elongation and salt-alkali treatment. The results of this research help understand the early growth regulation mechanism of G. barbadense in response to abiotic stress.Cun RuiCun RuiYuexin ZhangYapeng FanMingge HanMaohua DaiQinqin WangXiugui ChenXuke LuDelong WangShuai WangWenwei GaoJohn Z. YuWuwei YeWuwei YeFrontiers Media S.A.articleGossypium barbadensecell elongationDNA methylationhigh pH alkalinerespondingPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gossypium barbadense cell elongation DNA methylation high pH alkaline responding Plant culture SB1-1110
spellingShingle	Gossypium barbadense cell elongation DNA methylation high pH alkaline responding Plant culture SB1-1110 Cun Rui Cun Rui Yuexin Zhang Yapeng Fan Mingge Han Maohua Dai Qinqin Wang Xiugui Chen Xuke Lu Delong Wang Shuai Wang Wenwei Gao John Z. Yu Wuwei Ye Wuwei Ye Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
description	Gossypium barbadense is a cultivated cotton not only known for producing superior fiber but also for its salt and alkaline resistance. Here, we used Whole Genome Bisulfite Sequencing (WGBS) technology to map the cytosine methylation of the whole genome of the G. barbadense hypocotyl at single base resolution. The methylation sequencing results showed that the mapping rates of the three samples were 75.32, 77.54, and 77.94%, respectively. In addition, the Bisulfite Sequence (BS) conversion rate was 99.78%. Approximately 71.03, 53.87, and 6.26% of the cytosine were methylated at CG, CHG, and CHH sequence contexts, respectively. A comprehensive analysis of DNA methylation and transcriptome data showed that the methylation level of the promoter region was a positive correlation in the CHH context. Saline-alkaline stress was related to the methylation changes of many genes, transcription factors (TFs) and transposable elements (TEs), respectively. We explored the regulatory mechanism of DNA methylation in response to salt and alkaline stress during cotton hypocotyl elongation. Our data shed light into the relationship of methylation regulation at the germination stage of G. barbadense hypocotyl cell elongation and salt-alkali treatment. The results of this research help understand the early growth regulation mechanism of G. barbadense in response to abiotic stress.
format	article
author	Cun Rui Cun Rui Yuexin Zhang Yapeng Fan Mingge Han Maohua Dai Qinqin Wang Xiugui Chen Xuke Lu Delong Wang Shuai Wang Wenwei Gao John Z. Yu Wuwei Ye Wuwei Ye
author_facet	Cun Rui Cun Rui Yuexin Zhang Yapeng Fan Mingge Han Maohua Dai Qinqin Wang Xiugui Chen Xuke Lu Delong Wang Shuai Wang Wenwei Gao John Z. Yu Wuwei Ye Wuwei Ye
author_sort	Cun Rui
title	Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
title_short	Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
title_full	Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
title_fullStr	Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
title_full_unstemmed	Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress
title_sort	insight between the epigenetics and transcription responding of cotton hypocotyl cellular elongation under salt-alkaline stress
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/198a996e6ff4431097bd34dddb8fa380
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Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress

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