Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.

Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the presen...

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Autores principales:	Xiao-wu Zhong, Yong Zou, Shi-ping Liu, Qi-ying Yi, Cui-mei Hu, Chen Wang, Qing-you Xia, Ping Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/198d4efd345a412c9e6fc67a15cf14a0
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spelling	oai:doaj.org-article:198d4efd345a412c9e6fc67a15cf14a02021-11-18T08:53:12ZProteomic-based insight into Malpighian tubules of silkworm Bombyx mori.1932-620310.1371/journal.pone.0075731https://doaj.org/article/198d4efd345a412c9e6fc67a15cf14a02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098719/?tool=EBIhttps://doaj.org/toc/1932-6203Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.Xiao-wu ZhongYong ZouShi-ping LiuQi-ying YiCui-mei HuChen WangQing-you XiaPing ZhaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75731 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-wu Zhong Yong Zou Shi-ping Liu Qi-ying Yi Cui-mei Hu Chen Wang Qing-you Xia Ping Zhao Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
description	Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.
format	article
author	Xiao-wu Zhong Yong Zou Shi-ping Liu Qi-ying Yi Cui-mei Hu Chen Wang Qing-you Xia Ping Zhao
author_facet	Xiao-wu Zhong Yong Zou Shi-ping Liu Qi-ying Yi Cui-mei Hu Chen Wang Qing-you Xia Ping Zhao
author_sort	Xiao-wu Zhong
title	Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
title_short	Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
title_full	Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
title_fullStr	Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
title_full_unstemmed	Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.
title_sort	proteomic-based insight into malpighian tubules of silkworm bombyx mori.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/198d4efd345a412c9e6fc67a15cf14a0
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Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.

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