A model of atherosclerosis using nicotine with balloon overdilation in a porcine

Abstract Pigs are important experimental animals for cardiovascular research. Few porcine coronary atherosclerosis models have been developed; however, their induction requires more than six months. We developed a porcine coronary artery atherosclerosis model using nicotine injection with a balloon...

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Autores principales:	Munki Kim, Han Byul Kim, Dae Sung Park, Kyung Hoon Cho, Dae Young Hyun, Hae Jin Kee, Young Joon Hong, Myung Ho Jeong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/198e5f60e1824128b1d4e4ca5f3c0f3d
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spelling	oai:doaj.org-article:198e5f60e1824128b1d4e4ca5f3c0f3d2021-12-02T16:10:37ZA model of atherosclerosis using nicotine with balloon overdilation in a porcine10.1038/s41598-021-93229-12045-2322https://doaj.org/article/198e5f60e1824128b1d4e4ca5f3c0f3d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93229-1https://doaj.org/toc/2045-2322Abstract Pigs are important experimental animals for cardiovascular research. Few porcine coronary atherosclerosis models have been developed; however, their induction requires more than six months. We developed a porcine coronary artery atherosclerosis model using nicotine injection with a balloon overdilation. A coronary balloon was placed in the porcine coronary artery and overdilated to induce a mechanical injury. Nicotine was administrated via intramuscular injection every day, and changes in the coronary artery were observed after four weeks. Coronary angiography revealed nicotine injection with a balloon overdilation group showed narrowing of the coronary artery at the injury site. The combination of balloon and nicotine significantly increased the intimal hyperplasia in optical coherence tomography analysis. Proliferated tunica media were noted in the nicotine injection with balloon overdilation groups and lack of collagen was observed in the tunica media at eight weeks. Quantitative analysis showed increased smooth muscle actin alpha (SMA), cluster of differentiation 68 (CD68), and Krüppel-like factor 4 (KLF4) in the nicotine injection with balloon overdilation groups. Immunohistochemistry results showed CD68-positive cells displayed SMA- and KLF4-positive reactivity in the border zone of the intimal hyperplasia. Our results show that nicotine injection with balloon overdilation can induce atherosclerotic lesions within one month, which can serve as an alternative pig animal model for the development of coronary stents.Munki KimHan Byul KimDae Sung ParkKyung Hoon ChoDae Young HyunHae Jin KeeYoung Joon HongMyung Ho JeongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Munki Kim Han Byul Kim Dae Sung Park Kyung Hoon Cho Dae Young Hyun Hae Jin Kee Young Joon Hong Myung Ho Jeong A model of atherosclerosis using nicotine with balloon overdilation in a porcine
description	Abstract Pigs are important experimental animals for cardiovascular research. Few porcine coronary atherosclerosis models have been developed; however, their induction requires more than six months. We developed a porcine coronary artery atherosclerosis model using nicotine injection with a balloon overdilation. A coronary balloon was placed in the porcine coronary artery and overdilated to induce a mechanical injury. Nicotine was administrated via intramuscular injection every day, and changes in the coronary artery were observed after four weeks. Coronary angiography revealed nicotine injection with a balloon overdilation group showed narrowing of the coronary artery at the injury site. The combination of balloon and nicotine significantly increased the intimal hyperplasia in optical coherence tomography analysis. Proliferated tunica media were noted in the nicotine injection with balloon overdilation groups and lack of collagen was observed in the tunica media at eight weeks. Quantitative analysis showed increased smooth muscle actin alpha (SMA), cluster of differentiation 68 (CD68), and Krüppel-like factor 4 (KLF4) in the nicotine injection with balloon overdilation groups. Immunohistochemistry results showed CD68-positive cells displayed SMA- and KLF4-positive reactivity in the border zone of the intimal hyperplasia. Our results show that nicotine injection with balloon overdilation can induce atherosclerotic lesions within one month, which can serve as an alternative pig animal model for the development of coronary stents.
format	article
author	Munki Kim Han Byul Kim Dae Sung Park Kyung Hoon Cho Dae Young Hyun Hae Jin Kee Young Joon Hong Myung Ho Jeong
author_facet	Munki Kim Han Byul Kim Dae Sung Park Kyung Hoon Cho Dae Young Hyun Hae Jin Kee Young Joon Hong Myung Ho Jeong
author_sort	Munki Kim
title	A model of atherosclerosis using nicotine with balloon overdilation in a porcine
title_short	A model of atherosclerosis using nicotine with balloon overdilation in a porcine
title_full	A model of atherosclerosis using nicotine with balloon overdilation in a porcine
title_fullStr	A model of atherosclerosis using nicotine with balloon overdilation in a porcine
title_full_unstemmed	A model of atherosclerosis using nicotine with balloon overdilation in a porcine
title_sort	model of atherosclerosis using nicotine with balloon overdilation in a porcine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/198e5f60e1824128b1d4e4ca5f3c0f3d
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A model of atherosclerosis using nicotine with balloon overdilation in a porcine

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